Neuronal Cell-type Engineering by Transcriptional Activation

Gene activation with the CRISPR-Cas system has great implications in studying gene function, controlling cellular behavior, and modulating disease progression. In this review, we survey recent studies on targeted gene activation and multiplexed screening for inducing neuronal differentiation using C...

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Detalles Bibliográficos
Autores principales: Liu, Songlei, Striebel, Johannes, Pasquini, Giovanni, Ng, Alex H. M., Khoshakhlagh, Parastoo, Church, George M., Busskamp, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Genome Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525383/
https://www.ncbi.nlm.nih.gov/pubmed/34713262
http://dx.doi.org/10.3389/fgeed.2021.715697
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author Liu, Songlei
Striebel, Johannes
Pasquini, Giovanni
Ng, Alex H. M.
Khoshakhlagh, Parastoo
Church, George M.
Busskamp, Volker
author_facet Liu, Songlei
Striebel, Johannes
Pasquini, Giovanni
Ng, Alex H. M.
Khoshakhlagh, Parastoo
Church, George M.
Busskamp, Volker
author_sort Liu, Songlei
collection PubMed
description Gene activation with the CRISPR-Cas system has great implications in studying gene function, controlling cellular behavior, and modulating disease progression. In this review, we survey recent studies on targeted gene activation and multiplexed screening for inducing neuronal differentiation using CRISPR-Cas transcriptional activation (CRISPRa) and open reading frame (ORF) expression. Critical technical parameters of CRISPRa and ORF-based strategies for neuronal programming are presented and discussed. In addition, recent progress on in vivo applications of CRISPRa to the nervous system are highlighted. Overall, CRISPRa represents a valuable addition to the experimental toolbox for neuronal cell-type programming.
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spelling pubmed-85253832021-10-27 Neuronal Cell-type Engineering by Transcriptional Activation Liu, Songlei Striebel, Johannes Pasquini, Giovanni Ng, Alex H. M. Khoshakhlagh, Parastoo Church, George M. Busskamp, Volker Front Genome Ed Genome Editing Gene activation with the CRISPR-Cas system has great implications in studying gene function, controlling cellular behavior, and modulating disease progression. In this review, we survey recent studies on targeted gene activation and multiplexed screening for inducing neuronal differentiation using CRISPR-Cas transcriptional activation (CRISPRa) and open reading frame (ORF) expression. Critical technical parameters of CRISPRa and ORF-based strategies for neuronal programming are presented and discussed. In addition, recent progress on in vivo applications of CRISPRa to the nervous system are highlighted. Overall, CRISPRa represents a valuable addition to the experimental toolbox for neuronal cell-type programming. Frontiers Media S.A. 2021-09-01 /pmc/articles/PMC8525383/ /pubmed/34713262 http://dx.doi.org/10.3389/fgeed.2021.715697 Text en Copyright © 2021 Liu, Striebel, Pasquini, Ng, Khoshakhlagh, Church and Busskamp. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genome Editing
Liu, Songlei
Striebel, Johannes
Pasquini, Giovanni
Ng, Alex H. M.
Khoshakhlagh, Parastoo
Church, George M.
Busskamp, Volker
Neuronal Cell-type Engineering by Transcriptional Activation
title Neuronal Cell-type Engineering by Transcriptional Activation
title_full Neuronal Cell-type Engineering by Transcriptional Activation
title_fullStr Neuronal Cell-type Engineering by Transcriptional Activation
title_full_unstemmed Neuronal Cell-type Engineering by Transcriptional Activation
title_short Neuronal Cell-type Engineering by Transcriptional Activation
title_sort neuronal cell-type engineering by transcriptional activation
topic Genome Editing
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525383/
https://www.ncbi.nlm.nih.gov/pubmed/34713262
http://dx.doi.org/10.3389/fgeed.2021.715697
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AT khoshakhlaghparastoo neuronalcelltypeengineeringbytranscriptionalactivation
AT churchgeorgem neuronalcelltypeengineeringbytranscriptionalactivation
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