Cargando…
A Universal System of CRISPR/Cas9-Mediated Gene Targeting Using All-in-One Vector in Plants
Homologous recombination-mediated genome editing, also called gene targeting (GT), is an essential technique that allows precise modification of a target sequence, including introduction of point mutations, knock-in of a reporter gene, and/or swapping of a functional domain. However, due to its low...
Autores principales: | Nishizawa-Yokoi, Ayako, Mikami, Masafumi, Toki, Seiichi |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525384/ https://www.ncbi.nlm.nih.gov/pubmed/34713227 http://dx.doi.org/10.3389/fgeed.2020.604289 |
Ejemplares similares
-
Enhanced FnCas12a-Mediated Targeted Mutagenesis Using crRNA With Altered Target Length in Rice
por: Negishi, Katsuya, et al.
Publicado: (2020) -
A piggyBac‐mediated transgenesis system for the temporary expression of CRISPR/Cas9 in rice
por: Nishizawa‐Yokoi, Ayako, et al.
Publicado: (2021) -
Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants
por: Ohtsuki, Namie, et al.
Publicado: (2021) -
Mini-Review: Transgenerational CRISPR/Cas9 Gene Editing in Plants
por: Impens, Lennert, et al.
Publicado: (2022) -
CRISPR/Cas9 Mutagenesis by Translocation of Cas9 Protein Into Plant Cells via the Agrobacterium Type IV Secretion System
por: Schmitz, Daan J., et al.
Publicado: (2020)