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Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications

BACKGROUND: Diverse and unique bioactive neurotoxins known as conopeptides or conotoxins are produced by venomous marine cone snails. Currently, these small and stable molecules are of great importance as research tools and platforms for discovering new drugs and therapeutics. Therefore, the charact...

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Autores principales: Fouda, Maged M. A., Abdel-Wahab, Mohammed, Mohammadien, Amal, Germoush, Mousa O., Sarhan, Moustafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525892/
https://www.ncbi.nlm.nih.gov/pubmed/34712278
http://dx.doi.org/10.1590/1678-9199-JVATITD-2021-0023
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author Fouda, Maged M. A.
Abdel-Wahab, Mohammed
Mohammadien, Amal
Germoush, Mousa O.
Sarhan, Moustafa
author_facet Fouda, Maged M. A.
Abdel-Wahab, Mohammed
Mohammadien, Amal
Germoush, Mousa O.
Sarhan, Moustafa
author_sort Fouda, Maged M. A.
collection PubMed
description BACKGROUND: Diverse and unique bioactive neurotoxins known as conopeptides or conotoxins are produced by venomous marine cone snails. Currently, these small and stable molecules are of great importance as research tools and platforms for discovering new drugs and therapeutics. Therefore, the characterization of Conus venom is of great significance, especially for poorly studied species. METHODS: In this study, we used bioanalytical techniques to determine the venom profile and emphasize the functional composition of conopeptides in Conus taeniatus, a neglected worm-hunting cone snail. RESULTS: The proteomic analysis revealed that 84.0% of the venom proteins were between 500 and 4,000 Da, and 16.0% were > 4,000 Da. In C. taeniatus venom, 234 peptide fragments were identified and classified as conotoxin precursors or non-conotoxin proteins. In this process, 153 conotoxin precursors were identified and matched to 23 conotoxin precursors and hormone superfamilies. Notably, the four conotoxin superfamilies T (22.87%), O1 (17.65%), M (13.1%) and O2 (9.8%) were the most abundant peptides in C. taeniatus venom, accounting for 63.40% of the total conotoxin diversity. On the other hand, 48 non-conotoxin proteins were identified in the venom of C. taeniatus. Moreover, several possibly biologically active peptide matches were identified, and putative applications of the peptides were assigned. CONCLUSION: Our study showed that the composition of the C. taeniatus-derived proteome is comparable to that of other Conus species and contains an effective mix of toxins, ionic channel inhibitors and antimicrobials. Additionally, it provides a guidepost for identifying novel conopeptides from the venom of C. taeniatus and discovering conopeptides of potential pharmaceutical importance.
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spelling pubmed-85258922021-10-27 Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications Fouda, Maged M. A. Abdel-Wahab, Mohammed Mohammadien, Amal Germoush, Mousa O. Sarhan, Moustafa J Venom Anim Toxins Incl Trop Dis Research BACKGROUND: Diverse and unique bioactive neurotoxins known as conopeptides or conotoxins are produced by venomous marine cone snails. Currently, these small and stable molecules are of great importance as research tools and platforms for discovering new drugs and therapeutics. Therefore, the characterization of Conus venom is of great significance, especially for poorly studied species. METHODS: In this study, we used bioanalytical techniques to determine the venom profile and emphasize the functional composition of conopeptides in Conus taeniatus, a neglected worm-hunting cone snail. RESULTS: The proteomic analysis revealed that 84.0% of the venom proteins were between 500 and 4,000 Da, and 16.0% were > 4,000 Da. In C. taeniatus venom, 234 peptide fragments were identified and classified as conotoxin precursors or non-conotoxin proteins. In this process, 153 conotoxin precursors were identified and matched to 23 conotoxin precursors and hormone superfamilies. Notably, the four conotoxin superfamilies T (22.87%), O1 (17.65%), M (13.1%) and O2 (9.8%) were the most abundant peptides in C. taeniatus venom, accounting for 63.40% of the total conotoxin diversity. On the other hand, 48 non-conotoxin proteins were identified in the venom of C. taeniatus. Moreover, several possibly biologically active peptide matches were identified, and putative applications of the peptides were assigned. CONCLUSION: Our study showed that the composition of the C. taeniatus-derived proteome is comparable to that of other Conus species and contains an effective mix of toxins, ionic channel inhibitors and antimicrobials. Additionally, it provides a guidepost for identifying novel conopeptides from the venom of C. taeniatus and discovering conopeptides of potential pharmaceutical importance. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP 2021-10-18 /pmc/articles/PMC8525892/ /pubmed/34712278 http://dx.doi.org/10.1590/1678-9199-JVATITD-2021-0023 Text en https://creativecommons.org/licenses/by/4.0/© The Author(s). 2021 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Fouda, Maged M. A.
Abdel-Wahab, Mohammed
Mohammadien, Amal
Germoush, Mousa O.
Sarhan, Moustafa
Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications
title Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications
title_full Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications
title_fullStr Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications
title_full_unstemmed Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications
title_short Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications
title_sort proteomic analysis of red sea conus taeniatus venom reveals potential biological applications
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525892/
https://www.ncbi.nlm.nih.gov/pubmed/34712278
http://dx.doi.org/10.1590/1678-9199-JVATITD-2021-0023
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