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Network pharmacology and experimental investigation of Rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery

Rhizoma polygonati (Huangjing, RP) has been used for a long history with many chemical components in inducing anti-cancer, anti-aging, anti-diabetes, anti-fatigue, and more prevention of diseases or acts as nutrition sources in food. Here we investigated RP extract combination with kinase inhibitors...

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Autores principales: Xie, Yingqiu, Mu, Chengling, Kazybay, Bexultan, Sun, Qinglei, Kutzhanova, Aidana, Nazarbek, Guldan, Xu, Na, Nurtay, Lazzat, Wang, Qian, Amin, Amr, Li, Xugang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526013/
https://www.ncbi.nlm.nih.gov/pubmed/34662244
http://dx.doi.org/10.1080/10717544.2021.1977422
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author Xie, Yingqiu
Mu, Chengling
Kazybay, Bexultan
Sun, Qinglei
Kutzhanova, Aidana
Nazarbek, Guldan
Xu, Na
Nurtay, Lazzat
Wang, Qian
Amin, Amr
Li, Xugang
author_facet Xie, Yingqiu
Mu, Chengling
Kazybay, Bexultan
Sun, Qinglei
Kutzhanova, Aidana
Nazarbek, Guldan
Xu, Na
Nurtay, Lazzat
Wang, Qian
Amin, Amr
Li, Xugang
author_sort Xie, Yingqiu
collection PubMed
description Rhizoma polygonati (Huangjing, RP) has been used for a long history with many chemical components in inducing anti-cancer, anti-aging, anti-diabetes, anti-fatigue, and more prevention of diseases or acts as nutrition sources in food. Here we investigated RP extract combination with kinase inhibitors in anti-cell growth and blockade in pathways targeting kinases. Experimental investigation and network pharmacology analysis were applied to test the potent kinase-mediated signaling. Herbzyme activity was determined by substrate with optical density measurement. Extract of processed RP inhibits cell growth in a much greater manner than alone when applied in combination with inhibitors of mTOR or EGFR. Moreover, processing methods of RP from Mount Tai (RP-Mount Tai) play essential roles in herbzyme activity of phosphatase suggesting the interface is also essential, in addition to the chemical component. The network pharmacology analysis showed the chemical component and target networks involving AKT and mTOR, which is consistent with experimental validation. Finally, EGFR inhibitor could be associated with nano-extract of RP-Mount Tai but not significantly affects the phosphatase herbzyme activity in vitro. Thus the processed extract of RP-Mount Tai may play a dual role in the inhibition of cell proliferation signaling by both chemical component and nanoscale herbzyme of phosphatase activity to inhibit kinases including mTOR/AKT in potent drug delivery of kinase inhibitors.
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spelling pubmed-85260132021-10-20 Network pharmacology and experimental investigation of Rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery Xie, Yingqiu Mu, Chengling Kazybay, Bexultan Sun, Qinglei Kutzhanova, Aidana Nazarbek, Guldan Xu, Na Nurtay, Lazzat Wang, Qian Amin, Amr Li, Xugang Drug Deliv Research Article Rhizoma polygonati (Huangjing, RP) has been used for a long history with many chemical components in inducing anti-cancer, anti-aging, anti-diabetes, anti-fatigue, and more prevention of diseases or acts as nutrition sources in food. Here we investigated RP extract combination with kinase inhibitors in anti-cell growth and blockade in pathways targeting kinases. Experimental investigation and network pharmacology analysis were applied to test the potent kinase-mediated signaling. Herbzyme activity was determined by substrate with optical density measurement. Extract of processed RP inhibits cell growth in a much greater manner than alone when applied in combination with inhibitors of mTOR or EGFR. Moreover, processing methods of RP from Mount Tai (RP-Mount Tai) play essential roles in herbzyme activity of phosphatase suggesting the interface is also essential, in addition to the chemical component. The network pharmacology analysis showed the chemical component and target networks involving AKT and mTOR, which is consistent with experimental validation. Finally, EGFR inhibitor could be associated with nano-extract of RP-Mount Tai but not significantly affects the phosphatase herbzyme activity in vitro. Thus the processed extract of RP-Mount Tai may play a dual role in the inhibition of cell proliferation signaling by both chemical component and nanoscale herbzyme of phosphatase activity to inhibit kinases including mTOR/AKT in potent drug delivery of kinase inhibitors. Taylor & Francis 2021-10-18 /pmc/articles/PMC8526013/ /pubmed/34662244 http://dx.doi.org/10.1080/10717544.2021.1977422 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xie, Yingqiu
Mu, Chengling
Kazybay, Bexultan
Sun, Qinglei
Kutzhanova, Aidana
Nazarbek, Guldan
Xu, Na
Nurtay, Lazzat
Wang, Qian
Amin, Amr
Li, Xugang
Network pharmacology and experimental investigation of Rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery
title Network pharmacology and experimental investigation of Rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery
title_full Network pharmacology and experimental investigation of Rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery
title_fullStr Network pharmacology and experimental investigation of Rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery
title_full_unstemmed Network pharmacology and experimental investigation of Rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery
title_short Network pharmacology and experimental investigation of Rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery
title_sort network pharmacology and experimental investigation of rhizoma polygonati extract targeted kinase with herbzyme activity for potent drug delivery
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526013/
https://www.ncbi.nlm.nih.gov/pubmed/34662244
http://dx.doi.org/10.1080/10717544.2021.1977422
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