Cargando…
Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway
BACKGROUND: Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine Leptochloa chinensis in the pathogenesis of RCC. Meanwhile, this study also revealed the mo...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526199/ https://www.ncbi.nlm.nih.gov/pubmed/34675984 http://dx.doi.org/10.1155/2021/5122380 |
_version_ | 1784585830241665024 |
---|---|
author | Tan, Yongshun Li, Lingyun Liu, Hongyue Yu, Jiadong Wang, Qijun Lin, Qiuju |
author_facet | Tan, Yongshun Li, Lingyun Liu, Hongyue Yu, Jiadong Wang, Qijun Lin, Qiuju |
author_sort | Tan, Yongshun |
collection | PubMed |
description | BACKGROUND: Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine Leptochloa chinensis in the pathogenesis of RCC. Meanwhile, this study also revealed the molecular biological mechanism of its antitumor activity. METHODS: Human RCC 786-O cells were cultured in the RPMI-1640 medium, which contains different concentrations of Leptochloa chinensis (1,000, 3,000, and 9,000 μg/ml). MTT and flow cytometry assays were used to detect the viability of 786-O cells. Transwell and wound healing assays were used to detect cell metastasis. The protein expression was observed by western blot analysis. RESULTS: Leptochloa can inhibit cell proliferation and induce apoptosis in RCC 786-O cells. In addition, Leptochloa can weaken the migration and invasion of 786-O cells. More importantly, Leptochloa can block the mTOR pathway by inhibiting the protein expression of p-mTOR. Moreover, the high concentration of Leptochloa chinensis has a better inhibitory effect on 786-O cells. CONCLUSION: The traditional Chinese medicine Leptochloa chinensis inhibits the viability and metastasis of 786-O cells by blocking the mTOR pathway. |
format | Online Article Text |
id | pubmed-8526199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-85261992021-10-20 Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway Tan, Yongshun Li, Lingyun Liu, Hongyue Yu, Jiadong Wang, Qijun Lin, Qiuju Evid Based Complement Alternat Med Research Article BACKGROUND: Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine Leptochloa chinensis in the pathogenesis of RCC. Meanwhile, this study also revealed the molecular biological mechanism of its antitumor activity. METHODS: Human RCC 786-O cells were cultured in the RPMI-1640 medium, which contains different concentrations of Leptochloa chinensis (1,000, 3,000, and 9,000 μg/ml). MTT and flow cytometry assays were used to detect the viability of 786-O cells. Transwell and wound healing assays were used to detect cell metastasis. The protein expression was observed by western blot analysis. RESULTS: Leptochloa can inhibit cell proliferation and induce apoptosis in RCC 786-O cells. In addition, Leptochloa can weaken the migration and invasion of 786-O cells. More importantly, Leptochloa can block the mTOR pathway by inhibiting the protein expression of p-mTOR. Moreover, the high concentration of Leptochloa chinensis has a better inhibitory effect on 786-O cells. CONCLUSION: The traditional Chinese medicine Leptochloa chinensis inhibits the viability and metastasis of 786-O cells by blocking the mTOR pathway. Hindawi 2021-10-12 /pmc/articles/PMC8526199/ /pubmed/34675984 http://dx.doi.org/10.1155/2021/5122380 Text en Copyright © 2021 Yongshun Tan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tan, Yongshun Li, Lingyun Liu, Hongyue Yu, Jiadong Wang, Qijun Lin, Qiuju Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway |
title | Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway |
title_full | Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway |
title_fullStr | Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway |
title_full_unstemmed | Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway |
title_short | Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway |
title_sort | chinese medicine leptochloa chinensis inhibits the malignant behaviors of renal cell carcinoma 786-o cells by regulating the mtor pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526199/ https://www.ncbi.nlm.nih.gov/pubmed/34675984 http://dx.doi.org/10.1155/2021/5122380 |
work_keys_str_mv | AT tanyongshun chinesemedicineleptochloachinensisinhibitsthemalignantbehaviorsofrenalcellcarcinoma786ocellsbyregulatingthemtorpathway AT lilingyun chinesemedicineleptochloachinensisinhibitsthemalignantbehaviorsofrenalcellcarcinoma786ocellsbyregulatingthemtorpathway AT liuhongyue chinesemedicineleptochloachinensisinhibitsthemalignantbehaviorsofrenalcellcarcinoma786ocellsbyregulatingthemtorpathway AT yujiadong chinesemedicineleptochloachinensisinhibitsthemalignantbehaviorsofrenalcellcarcinoma786ocellsbyregulatingthemtorpathway AT wangqijun chinesemedicineleptochloachinensisinhibitsthemalignantbehaviorsofrenalcellcarcinoma786ocellsbyregulatingthemtorpathway AT linqiuju chinesemedicineleptochloachinensisinhibitsthemalignantbehaviorsofrenalcellcarcinoma786ocellsbyregulatingthemtorpathway |