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Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture

Fibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes...

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Autores principales: Yaoita, Eishin, Nameta, Masaaki, Yoshida, Yutaka, Fujinaka, Hidehiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526483/
https://www.ncbi.nlm.nih.gov/pubmed/34309730
http://dx.doi.org/10.1007/s00441-021-03511-x
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author Yaoita, Eishin
Nameta, Masaaki
Yoshida, Yutaka
Fujinaka, Hidehiko
author_facet Yaoita, Eishin
Nameta, Masaaki
Yoshida, Yutaka
Fujinaka, Hidehiko
author_sort Yaoita, Eishin
collection PubMed
description Fibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. These findings show that FGF2 makes podocytes to transition from the quiescent state into the cell cycle and change their morphology due to enhanced motility, and that the culture system in this study is useful for analyzing the pathological changes of podocytes in vivo. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00441-021-03511-x.
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spelling pubmed-85264832021-11-04 Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture Yaoita, Eishin Nameta, Masaaki Yoshida, Yutaka Fujinaka, Hidehiko Cell Tissue Res Regular Article Fibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. These findings show that FGF2 makes podocytes to transition from the quiescent state into the cell cycle and change their morphology due to enhanced motility, and that the culture system in this study is useful for analyzing the pathological changes of podocytes in vivo. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00441-021-03511-x. Springer Berlin Heidelberg 2021-07-26 2021 /pmc/articles/PMC8526483/ /pubmed/34309730 http://dx.doi.org/10.1007/s00441-021-03511-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Regular Article
Yaoita, Eishin
Nameta, Masaaki
Yoshida, Yutaka
Fujinaka, Hidehiko
Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture
title Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture
title_full Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture
title_fullStr Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture
title_full_unstemmed Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture
title_short Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture
title_sort dynamic changes of podocytes caused by fibroblast growth factor 2 in culture
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526483/
https://www.ncbi.nlm.nih.gov/pubmed/34309730
http://dx.doi.org/10.1007/s00441-021-03511-x
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