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A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation

Circular RNA (circRNA) is a class of endogenous non-coding RNAs without 5′ and 3′ ends; an increasing number of studies show that circRNA is involved in skeletal muscle development. From our previous sequencing data, the circRNAome in breast muscle of two chicken lines with a distinct rate of muscle...

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Autores principales: Shen, Xiaoxu, Wei, Yuanhang, Liu, Wei, You, Guishuang, Tang, Shuyue, Su, Zhenyu, Du, Mingxin, He, Jian, Zhao, Jing, Tian, Yongtong, Zhang, Yao, Ma, Menggen, Zhu, Qing, Yin, Huadong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526564/
https://www.ncbi.nlm.nih.gov/pubmed/34692701
http://dx.doi.org/10.3389/fcell.2021.748844
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author Shen, Xiaoxu
Wei, Yuanhang
Liu, Wei
You, Guishuang
Tang, Shuyue
Su, Zhenyu
Du, Mingxin
He, Jian
Zhao, Jing
Tian, Yongtong
Zhang, Yao
Ma, Menggen
Zhu, Qing
Yin, Huadong
author_facet Shen, Xiaoxu
Wei, Yuanhang
Liu, Wei
You, Guishuang
Tang, Shuyue
Su, Zhenyu
Du, Mingxin
He, Jian
Zhao, Jing
Tian, Yongtong
Zhang, Yao
Ma, Menggen
Zhu, Qing
Yin, Huadong
author_sort Shen, Xiaoxu
collection PubMed
description Circular RNA (circRNA) is a class of endogenous non-coding RNAs without 5′ and 3′ ends; an increasing number of studies show that circRNA is involved in skeletal muscle development. From our previous sequencing data, the circRNAome in breast muscle of two chicken lines with a distinct rate of muscle development, which included a fast muscle growing broiler (FMGB) and a slow muscle growing layer (SMGL), we found a novel differentially expressed circRNA generated by intersectin 2 (ITSN2) gene (named circITSN2). We verified that circITSN2 is a skeletal muscle-enriched circRNA that promotes chicken primary myoblast (CPM) proliferation and differentiation. Further molecular mechanism analysis of circITSN2 in chicken myogenesis was performed, and we found circITSN2 directly targeting miR-218-5p. Besides, miR-218-5p inhibits CPM proliferation and differentiation, which is contrary to circITSN2. Commonly, circRNAs act as a miRNA sponge to alleviate the inhibition of miRNAs on mRNAs. Thus, we also identified that a downstream gene LIM domain 7 (LMO7) was inhibited by miR-218-5p, while circITSN2 could block the inhibitory effect of miR-218-5p by targeting it. Functional analysis revealed that LMO7 also accelerates CPM proliferation and differentiation, which was similar to circITSN2 but contrary to miR-218-5p. Taken together, these results suggested that circITSN2 promotes chicken embryonic skeletal muscle development via relieving the inhibition of miR-218-5p on LMO7. Our findings revealed a novel circITSN2/miR-218-5p/LMO7 axis in chicken embryonic skeletal muscle development, which expands our understanding of the complex muscle development regulatory network.
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spelling pubmed-85265642021-10-21 A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation Shen, Xiaoxu Wei, Yuanhang Liu, Wei You, Guishuang Tang, Shuyue Su, Zhenyu Du, Mingxin He, Jian Zhao, Jing Tian, Yongtong Zhang, Yao Ma, Menggen Zhu, Qing Yin, Huadong Front Cell Dev Biol Cell and Developmental Biology Circular RNA (circRNA) is a class of endogenous non-coding RNAs without 5′ and 3′ ends; an increasing number of studies show that circRNA is involved in skeletal muscle development. From our previous sequencing data, the circRNAome in breast muscle of two chicken lines with a distinct rate of muscle development, which included a fast muscle growing broiler (FMGB) and a slow muscle growing layer (SMGL), we found a novel differentially expressed circRNA generated by intersectin 2 (ITSN2) gene (named circITSN2). We verified that circITSN2 is a skeletal muscle-enriched circRNA that promotes chicken primary myoblast (CPM) proliferation and differentiation. Further molecular mechanism analysis of circITSN2 in chicken myogenesis was performed, and we found circITSN2 directly targeting miR-218-5p. Besides, miR-218-5p inhibits CPM proliferation and differentiation, which is contrary to circITSN2. Commonly, circRNAs act as a miRNA sponge to alleviate the inhibition of miRNAs on mRNAs. Thus, we also identified that a downstream gene LIM domain 7 (LMO7) was inhibited by miR-218-5p, while circITSN2 could block the inhibitory effect of miR-218-5p by targeting it. Functional analysis revealed that LMO7 also accelerates CPM proliferation and differentiation, which was similar to circITSN2 but contrary to miR-218-5p. Taken together, these results suggested that circITSN2 promotes chicken embryonic skeletal muscle development via relieving the inhibition of miR-218-5p on LMO7. Our findings revealed a novel circITSN2/miR-218-5p/LMO7 axis in chicken embryonic skeletal muscle development, which expands our understanding of the complex muscle development regulatory network. Frontiers Media S.A. 2021-10-06 /pmc/articles/PMC8526564/ /pubmed/34692701 http://dx.doi.org/10.3389/fcell.2021.748844 Text en Copyright © 2021 Shen, Wei, Liu, You, Tang, Su, Du, He, Zhao, Tian, Zhang, Ma, Zhu and Yin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Shen, Xiaoxu
Wei, Yuanhang
Liu, Wei
You, Guishuang
Tang, Shuyue
Su, Zhenyu
Du, Mingxin
He, Jian
Zhao, Jing
Tian, Yongtong
Zhang, Yao
Ma, Menggen
Zhu, Qing
Yin, Huadong
A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation
title A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation
title_full A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation
title_fullStr A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation
title_full_unstemmed A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation
title_short A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation
title_sort novel circular rna circitsn2 targets the mir-218-5p/lmo7 axis to promote chicken embryonic myoblast proliferation and differentiation
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526564/
https://www.ncbi.nlm.nih.gov/pubmed/34692701
http://dx.doi.org/10.3389/fcell.2021.748844
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