Cargando…
High-coverage metabolomics uncovers microbiota-driven biochemical landscape of interorgan transport and gut-brain communication in mice
The mammalian gut harbors a complex and dynamic microbial ecosystem: the microbiota. While emerging studies support that microbiota regulates brain function with a few molecular cues suggested, the overall biochemical landscape of the “microbiota-gut-brain axis” remains largely unclear. Here we use...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526691/ https://www.ncbi.nlm.nih.gov/pubmed/34667167 http://dx.doi.org/10.1038/s41467-021-26209-8 |
_version_ | 1784585915378696192 |
---|---|
author | Lai, Yunjia Liu, Chih-Wei Yang, Yifei Hsiao, Yun-Chung Ru, Hongyu Lu, Kun |
author_facet | Lai, Yunjia Liu, Chih-Wei Yang, Yifei Hsiao, Yun-Chung Ru, Hongyu Lu, Kun |
author_sort | Lai, Yunjia |
collection | PubMed |
description | The mammalian gut harbors a complex and dynamic microbial ecosystem: the microbiota. While emerging studies support that microbiota regulates brain function with a few molecular cues suggested, the overall biochemical landscape of the “microbiota-gut-brain axis” remains largely unclear. Here we use high-coverage metabolomics to comparatively profile feces, blood sera, and cerebral cortical brain tissues of germ-free C57BL/6 mice and their age-matched conventionally raised counterparts. Results revealed for all three matrices metabolomic signatures owing to microbiota, yielding hundreds of identified metabolites including 533 altered for feces, 231 for sera, and 58 for brain with numerous significantly enriched pathways involving aromatic amino acids and neurotransmitters. Multicompartmental comparative analyses single out microbiota-derived metabolites potentially implicated in interorgan transport and the gut-brain axis, as exemplified by indoxyl sulfate and trimethylamine-N-oxide. Gender-specific characteristics of these landscapes are discussed. Our findings may be valuable for future research probing microbial influences on host metabolism and gut-brain communication. |
format | Online Article Text |
id | pubmed-8526691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85266912021-11-15 High-coverage metabolomics uncovers microbiota-driven biochemical landscape of interorgan transport and gut-brain communication in mice Lai, Yunjia Liu, Chih-Wei Yang, Yifei Hsiao, Yun-Chung Ru, Hongyu Lu, Kun Nat Commun Article The mammalian gut harbors a complex and dynamic microbial ecosystem: the microbiota. While emerging studies support that microbiota regulates brain function with a few molecular cues suggested, the overall biochemical landscape of the “microbiota-gut-brain axis” remains largely unclear. Here we use high-coverage metabolomics to comparatively profile feces, blood sera, and cerebral cortical brain tissues of germ-free C57BL/6 mice and their age-matched conventionally raised counterparts. Results revealed for all three matrices metabolomic signatures owing to microbiota, yielding hundreds of identified metabolites including 533 altered for feces, 231 for sera, and 58 for brain with numerous significantly enriched pathways involving aromatic amino acids and neurotransmitters. Multicompartmental comparative analyses single out microbiota-derived metabolites potentially implicated in interorgan transport and the gut-brain axis, as exemplified by indoxyl sulfate and trimethylamine-N-oxide. Gender-specific characteristics of these landscapes are discussed. Our findings may be valuable for future research probing microbial influences on host metabolism and gut-brain communication. Nature Publishing Group UK 2021-10-19 /pmc/articles/PMC8526691/ /pubmed/34667167 http://dx.doi.org/10.1038/s41467-021-26209-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Lai, Yunjia Liu, Chih-Wei Yang, Yifei Hsiao, Yun-Chung Ru, Hongyu Lu, Kun High-coverage metabolomics uncovers microbiota-driven biochemical landscape of interorgan transport and gut-brain communication in mice |
title | High-coverage metabolomics uncovers microbiota-driven biochemical landscape of interorgan transport and gut-brain communication in mice |
title_full | High-coverage metabolomics uncovers microbiota-driven biochemical landscape of interorgan transport and gut-brain communication in mice |
title_fullStr | High-coverage metabolomics uncovers microbiota-driven biochemical landscape of interorgan transport and gut-brain communication in mice |
title_full_unstemmed | High-coverage metabolomics uncovers microbiota-driven biochemical landscape of interorgan transport and gut-brain communication in mice |
title_short | High-coverage metabolomics uncovers microbiota-driven biochemical landscape of interorgan transport and gut-brain communication in mice |
title_sort | high-coverage metabolomics uncovers microbiota-driven biochemical landscape of interorgan transport and gut-brain communication in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526691/ https://www.ncbi.nlm.nih.gov/pubmed/34667167 http://dx.doi.org/10.1038/s41467-021-26209-8 |
work_keys_str_mv | AT laiyunjia highcoveragemetabolomicsuncoversmicrobiotadrivenbiochemicallandscapeofinterorgantransportandgutbraincommunicationinmice AT liuchihwei highcoveragemetabolomicsuncoversmicrobiotadrivenbiochemicallandscapeofinterorgantransportandgutbraincommunicationinmice AT yangyifei highcoveragemetabolomicsuncoversmicrobiotadrivenbiochemicallandscapeofinterorgantransportandgutbraincommunicationinmice AT hsiaoyunchung highcoveragemetabolomicsuncoversmicrobiotadrivenbiochemicallandscapeofinterorgantransportandgutbraincommunicationinmice AT ruhongyu highcoveragemetabolomicsuncoversmicrobiotadrivenbiochemicallandscapeofinterorgantransportandgutbraincommunicationinmice AT lukun highcoveragemetabolomicsuncoversmicrobiotadrivenbiochemicallandscapeofinterorgantransportandgutbraincommunicationinmice |