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A Novel Endothelial Damage Inhibitor Reduces Oxidative Stress and Improves Cellular Integrity in Radial Artery Grafts for Coronary Artery Bypass

The radial artery (RA) is a frequently used conduit in coronary artery bypass grafting (CABG). Endothelial injury incurred during graft harvesting promotes oxidative damage, which leads to graft disease and graft failure. We evaluated the protective effect of DuraGraft®, an endothelial damage inhibi...

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Autores principales: Aschacher, Thomas, Baranyi, Ulrike, Aschacher, Olivia, Eichmair, Eva, Messner, Barbara, Zimpfer, Daniel, Moayedifar, Roxana, Laufer, Guenther, Emmert, Maximilian Y., Sandner, Sigrid E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527012/
https://www.ncbi.nlm.nih.gov/pubmed/34692789
http://dx.doi.org/10.3389/fcvm.2021.736503
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author Aschacher, Thomas
Baranyi, Ulrike
Aschacher, Olivia
Eichmair, Eva
Messner, Barbara
Zimpfer, Daniel
Moayedifar, Roxana
Laufer, Guenther
Emmert, Maximilian Y.
Sandner, Sigrid E.
author_facet Aschacher, Thomas
Baranyi, Ulrike
Aschacher, Olivia
Eichmair, Eva
Messner, Barbara
Zimpfer, Daniel
Moayedifar, Roxana
Laufer, Guenther
Emmert, Maximilian Y.
Sandner, Sigrid E.
author_sort Aschacher, Thomas
collection PubMed
description The radial artery (RA) is a frequently used conduit in coronary artery bypass grafting (CABG). Endothelial injury incurred during graft harvesting promotes oxidative damage, which leads to graft disease and graft failure. We evaluated the protective effect of DuraGraft®, an endothelial damage inhibitor (EDI), on RA grafts. We further compared the protective effect of the EDI between RA grafts and saphenous vein grafts (SVG). Samples of RA (n = 10) and SVG (n = 13) from 23 patients undergoing CABG were flushed and preserved with either EDI or heparinized Ringer's lactate solution (RL). The effect of EDI vs. RL on endothelial damage was evaluated ex vivo and in vitro using histological analysis, immunofluorescence staining, Western blot, and scanning electron microscopy. EDI-treated RA grafts showed a significant reduction of endothelial and sub-endothelial damage. Lower level of reactive oxygen species (ROS) after EDI treatment was correlated with a reduction of hypoxic damage (eNOS and Caveolin-1) and significant increase of oxidation-reduction potential. Additionally, an increased expression of TGFβ, PDGFα/β, and HO-1 which are indicative for vascular protective function were observed after EDI exposure. EDI treatment preserves functionality and integrity of endothelial and intimal cells. Therefore, EDI may have the potential to reduce the occurrence of graft disease and failure in RA grafts in patients undergoing CABG.
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spelling pubmed-85270122021-10-21 A Novel Endothelial Damage Inhibitor Reduces Oxidative Stress and Improves Cellular Integrity in Radial Artery Grafts for Coronary Artery Bypass Aschacher, Thomas Baranyi, Ulrike Aschacher, Olivia Eichmair, Eva Messner, Barbara Zimpfer, Daniel Moayedifar, Roxana Laufer, Guenther Emmert, Maximilian Y. Sandner, Sigrid E. Front Cardiovasc Med Cardiovascular Medicine The radial artery (RA) is a frequently used conduit in coronary artery bypass grafting (CABG). Endothelial injury incurred during graft harvesting promotes oxidative damage, which leads to graft disease and graft failure. We evaluated the protective effect of DuraGraft®, an endothelial damage inhibitor (EDI), on RA grafts. We further compared the protective effect of the EDI between RA grafts and saphenous vein grafts (SVG). Samples of RA (n = 10) and SVG (n = 13) from 23 patients undergoing CABG were flushed and preserved with either EDI or heparinized Ringer's lactate solution (RL). The effect of EDI vs. RL on endothelial damage was evaluated ex vivo and in vitro using histological analysis, immunofluorescence staining, Western blot, and scanning electron microscopy. EDI-treated RA grafts showed a significant reduction of endothelial and sub-endothelial damage. Lower level of reactive oxygen species (ROS) after EDI treatment was correlated with a reduction of hypoxic damage (eNOS and Caveolin-1) and significant increase of oxidation-reduction potential. Additionally, an increased expression of TGFβ, PDGFα/β, and HO-1 which are indicative for vascular protective function were observed after EDI exposure. EDI treatment preserves functionality and integrity of endothelial and intimal cells. Therefore, EDI may have the potential to reduce the occurrence of graft disease and failure in RA grafts in patients undergoing CABG. Frontiers Media S.A. 2021-10-06 /pmc/articles/PMC8527012/ /pubmed/34692789 http://dx.doi.org/10.3389/fcvm.2021.736503 Text en Copyright © 2021 Aschacher, Baranyi, Aschacher, Eichmair, Messner, Zimpfer, Moayedifar, Laufer, Emmert and Sandner. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Aschacher, Thomas
Baranyi, Ulrike
Aschacher, Olivia
Eichmair, Eva
Messner, Barbara
Zimpfer, Daniel
Moayedifar, Roxana
Laufer, Guenther
Emmert, Maximilian Y.
Sandner, Sigrid E.
A Novel Endothelial Damage Inhibitor Reduces Oxidative Stress and Improves Cellular Integrity in Radial Artery Grafts for Coronary Artery Bypass
title A Novel Endothelial Damage Inhibitor Reduces Oxidative Stress and Improves Cellular Integrity in Radial Artery Grafts for Coronary Artery Bypass
title_full A Novel Endothelial Damage Inhibitor Reduces Oxidative Stress and Improves Cellular Integrity in Radial Artery Grafts for Coronary Artery Bypass
title_fullStr A Novel Endothelial Damage Inhibitor Reduces Oxidative Stress and Improves Cellular Integrity in Radial Artery Grafts for Coronary Artery Bypass
title_full_unstemmed A Novel Endothelial Damage Inhibitor Reduces Oxidative Stress and Improves Cellular Integrity in Radial Artery Grafts for Coronary Artery Bypass
title_short A Novel Endothelial Damage Inhibitor Reduces Oxidative Stress and Improves Cellular Integrity in Radial Artery Grafts for Coronary Artery Bypass
title_sort novel endothelial damage inhibitor reduces oxidative stress and improves cellular integrity in radial artery grafts for coronary artery bypass
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527012/
https://www.ncbi.nlm.nih.gov/pubmed/34692789
http://dx.doi.org/10.3389/fcvm.2021.736503
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