Cargando…
Characterization of IncHI1B Plasmids Encoding Efflux Pump TmexCD2-ToprJ2 in Carbapenem-Resistant Klebsiella variicola, Klebsiella quasipneumoniae, and Klebsiella michiganensis Strains
Tigecycline serves as one of the last-resort antibiotics to treat severe infections caused by carbapenem-resistant Enterobacterales. Recently, a novel plasmid-mediated resistance-nodulation-division (RND)-type efflux pump gene cluster, TmexCD1-ToprJ1, and its variants, TmexCD2-ToprJ2 and TmexCD3-Top...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527040/ https://www.ncbi.nlm.nih.gov/pubmed/34691010 http://dx.doi.org/10.3389/fmicb.2021.759208 |
_version_ | 1784585997009289216 |
---|---|
author | Wang, Yujiao Zhu, Bo Liu, Min Dong, Xiutao Ma, Jianping Li, Xiaofeng Cheng, Fang Guo, Jianzhuang Lu, Sumei Wan, Furong Hao, Yingying Ma, Wanshan Hao, Mingju Chen, Liang |
author_facet | Wang, Yujiao Zhu, Bo Liu, Min Dong, Xiutao Ma, Jianping Li, Xiaofeng Cheng, Fang Guo, Jianzhuang Lu, Sumei Wan, Furong Hao, Yingying Ma, Wanshan Hao, Mingju Chen, Liang |
author_sort | Wang, Yujiao |
collection | PubMed |
description | Tigecycline serves as one of the last-resort antibiotics to treat severe infections caused by carbapenem-resistant Enterobacterales. Recently, a novel plasmid-mediated resistance-nodulation-division (RND)-type efflux pump gene cluster, TmexCD1-ToprJ1, and its variants, TmexCD2-ToprJ2 and TmexCD3-ToprJ3, encoding tetracyclines and tigecycline resistance, were revealed. In this study, we reported three TmexCD2-ToprJ2-harboring Klebsiella species strains, collected from two teaching tertiary hospitals in China, including one K. quasipneumoniae, one K. variicola, and one K. michiganensis. The three strains were characterized by antimicrobial susceptibility testing (AST), conjugation assay, WGS, and bioinformatics analysis. AST showed that K. variicola and K. quasipneumoniae strains were resistant to tigecycline with MIC values of 4μg/ml, whereas the K. michiganensis was susceptible to tigecycline with an MIC value of 1μg/ml. The TmexCD2-ToprJ2 clusters were located on three similar IncHI1B plasmids, of which two co-harbored the metallo-β-lactamase gene bla(NDM-1). Conjugation experiments showed that all three plasmids were capable of self-transfer via conjugation. Our results showed, for the first time, that this novel plasmid-mediated tigecycline resistance mechanism TmexCD2-ToprJ2 has spread into different Klebsiella species, and clinical susceptibility testing may fail to detect. The co-occurrence of bla(NDM-1) and TmexCD2-ToprJ2 in the same plasmid is of particular public health concern as the convergence of “mosaic” plasmids can confer both tigecycline and carbapenem resistance. Its further spread into other clinical high-risk Klebsiella clones will likely exacerbate the antimicrobial resistance crisis. A close monitoring of the dissemination of TmexCD-ToprJ encoding resistance should be considered. |
format | Online Article Text |
id | pubmed-8527040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85270402021-10-21 Characterization of IncHI1B Plasmids Encoding Efflux Pump TmexCD2-ToprJ2 in Carbapenem-Resistant Klebsiella variicola, Klebsiella quasipneumoniae, and Klebsiella michiganensis Strains Wang, Yujiao Zhu, Bo Liu, Min Dong, Xiutao Ma, Jianping Li, Xiaofeng Cheng, Fang Guo, Jianzhuang Lu, Sumei Wan, Furong Hao, Yingying Ma, Wanshan Hao, Mingju Chen, Liang Front Microbiol Microbiology Tigecycline serves as one of the last-resort antibiotics to treat severe infections caused by carbapenem-resistant Enterobacterales. Recently, a novel plasmid-mediated resistance-nodulation-division (RND)-type efflux pump gene cluster, TmexCD1-ToprJ1, and its variants, TmexCD2-ToprJ2 and TmexCD3-ToprJ3, encoding tetracyclines and tigecycline resistance, were revealed. In this study, we reported three TmexCD2-ToprJ2-harboring Klebsiella species strains, collected from two teaching tertiary hospitals in China, including one K. quasipneumoniae, one K. variicola, and one K. michiganensis. The three strains were characterized by antimicrobial susceptibility testing (AST), conjugation assay, WGS, and bioinformatics analysis. AST showed that K. variicola and K. quasipneumoniae strains were resistant to tigecycline with MIC values of 4μg/ml, whereas the K. michiganensis was susceptible to tigecycline with an MIC value of 1μg/ml. The TmexCD2-ToprJ2 clusters were located on three similar IncHI1B plasmids, of which two co-harbored the metallo-β-lactamase gene bla(NDM-1). Conjugation experiments showed that all three plasmids were capable of self-transfer via conjugation. Our results showed, for the first time, that this novel plasmid-mediated tigecycline resistance mechanism TmexCD2-ToprJ2 has spread into different Klebsiella species, and clinical susceptibility testing may fail to detect. The co-occurrence of bla(NDM-1) and TmexCD2-ToprJ2 in the same plasmid is of particular public health concern as the convergence of “mosaic” plasmids can confer both tigecycline and carbapenem resistance. Its further spread into other clinical high-risk Klebsiella clones will likely exacerbate the antimicrobial resistance crisis. A close monitoring of the dissemination of TmexCD-ToprJ encoding resistance should be considered. Frontiers Media S.A. 2021-10-06 /pmc/articles/PMC8527040/ /pubmed/34691010 http://dx.doi.org/10.3389/fmicb.2021.759208 Text en Copyright © 2021 Wang, Zhu, Liu, Dong, Ma, Li, Cheng, Guo, Lu, Wan, Hao, Ma, Hao and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Wang, Yujiao Zhu, Bo Liu, Min Dong, Xiutao Ma, Jianping Li, Xiaofeng Cheng, Fang Guo, Jianzhuang Lu, Sumei Wan, Furong Hao, Yingying Ma, Wanshan Hao, Mingju Chen, Liang Characterization of IncHI1B Plasmids Encoding Efflux Pump TmexCD2-ToprJ2 in Carbapenem-Resistant Klebsiella variicola, Klebsiella quasipneumoniae, and Klebsiella michiganensis Strains |
title | Characterization of IncHI1B Plasmids Encoding Efflux Pump TmexCD2-ToprJ2 in Carbapenem-Resistant Klebsiella variicola, Klebsiella quasipneumoniae, and Klebsiella michiganensis Strains |
title_full | Characterization of IncHI1B Plasmids Encoding Efflux Pump TmexCD2-ToprJ2 in Carbapenem-Resistant Klebsiella variicola, Klebsiella quasipneumoniae, and Klebsiella michiganensis Strains |
title_fullStr | Characterization of IncHI1B Plasmids Encoding Efflux Pump TmexCD2-ToprJ2 in Carbapenem-Resistant Klebsiella variicola, Klebsiella quasipneumoniae, and Klebsiella michiganensis Strains |
title_full_unstemmed | Characterization of IncHI1B Plasmids Encoding Efflux Pump TmexCD2-ToprJ2 in Carbapenem-Resistant Klebsiella variicola, Klebsiella quasipneumoniae, and Klebsiella michiganensis Strains |
title_short | Characterization of IncHI1B Plasmids Encoding Efflux Pump TmexCD2-ToprJ2 in Carbapenem-Resistant Klebsiella variicola, Klebsiella quasipneumoniae, and Klebsiella michiganensis Strains |
title_sort | characterization of inchi1b plasmids encoding efflux pump tmexcd2-toprj2 in carbapenem-resistant klebsiella variicola, klebsiella quasipneumoniae, and klebsiella michiganensis strains |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527040/ https://www.ncbi.nlm.nih.gov/pubmed/34691010 http://dx.doi.org/10.3389/fmicb.2021.759208 |
work_keys_str_mv | AT wangyujiao characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT zhubo characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT liumin characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT dongxiutao characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT majianping characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT lixiaofeng characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT chengfang characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT guojianzhuang characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT lusumei characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT wanfurong characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT haoyingying characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT mawanshan characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT haomingju characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains AT chenliang characterizationofinchi1bplasmidsencodingeffluxpumptmexcd2toprj2incarbapenemresistantklebsiellavariicolaklebsiellaquasipneumoniaeandklebsiellamichiganensisstrains |