Cargando…
MBD2 as a Potential Novel Biomarker for Identifying Severe Asthma With Different Endotypes
Background: Studies have shown that methyl-CpG binding domain protein 2 (MBD2) expression is significantly elevated in a neutrophil-dominant severe asthma mouse model. It also regulates Th17 cell differentiation. The objective of this study was to investigate the relationship between serum MBD2 leve...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527858/ https://www.ncbi.nlm.nih.gov/pubmed/34692717 http://dx.doi.org/10.3389/fmed.2021.693605 |
_version_ | 1784586153948610560 |
---|---|
author | Chen, Zhifeng Yuan, Yu He, Yi Wasti, Binaya Duan, Wentao Jia, Jingsi Li, Danhong Xiao, Bing Zhang, Dongshan Ma, Libing Li, Jianmin Liu, Yi Zeng, Qingping Xiang, Xudong Zhang, Xiufeng Liu, Shaokun |
author_facet | Chen, Zhifeng Yuan, Yu He, Yi Wasti, Binaya Duan, Wentao Jia, Jingsi Li, Danhong Xiao, Bing Zhang, Dongshan Ma, Libing Li, Jianmin Liu, Yi Zeng, Qingping Xiang, Xudong Zhang, Xiufeng Liu, Shaokun |
author_sort | Chen, Zhifeng |
collection | PubMed |
description | Background: Studies have shown that methyl-CpG binding domain protein 2 (MBD2) expression is significantly elevated in a neutrophil-dominant severe asthma mouse model. It also regulates Th17 cell differentiation. The objective of this study was to investigate the relationship between serum MBD2 levels in patients with severe asthma with different endotypes. Methods: Eligible adults with confirmed asthma (n = 63) underwent a clinical assessment, asthma control test and pulmonary function test and were classified as having mild, moderate or severe asthma. Severe asthma endotypes were defined according to the percentage of Th2 and Th17 cells in the peripheral blood and by the type of inflammation. The percentage of Th2 and Th17 cells in the peripheral blood was determined by flow cytometry. Serum MBD2, eosinophilic cationic protein and myeloperoxidase were measured by enzyme-linked immunosorbent assay. Correlations of MBD2 expression with clinical parameters were evaluated using Spearman's rank correlation analysis. Results: Serum MBD2 levels were upregulated in patients with severe asthma compared to healthy controls and patients with mild to moderate asthma. MBD2 was also significantly increased in patients with Th17 severe asthma compared to patients with type 2 severe asthma. Furthermore, MBD2 was positively correlated with MPO and Th17 cells but negatively correlated with ECP and Th2 cells in patients with severe asthma. Conclusions: These findings suggest that serum MBD2 may be a potential new biomarker for identifying severe asthma, Th17 severe asthma and the type of airway inflammation. However, these findings are still preliminary and need to be further investigated. |
format | Online Article Text |
id | pubmed-8527858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85278582021-10-21 MBD2 as a Potential Novel Biomarker for Identifying Severe Asthma With Different Endotypes Chen, Zhifeng Yuan, Yu He, Yi Wasti, Binaya Duan, Wentao Jia, Jingsi Li, Danhong Xiao, Bing Zhang, Dongshan Ma, Libing Li, Jianmin Liu, Yi Zeng, Qingping Xiang, Xudong Zhang, Xiufeng Liu, Shaokun Front Med (Lausanne) Medicine Background: Studies have shown that methyl-CpG binding domain protein 2 (MBD2) expression is significantly elevated in a neutrophil-dominant severe asthma mouse model. It also regulates Th17 cell differentiation. The objective of this study was to investigate the relationship between serum MBD2 levels in patients with severe asthma with different endotypes. Methods: Eligible adults with confirmed asthma (n = 63) underwent a clinical assessment, asthma control test and pulmonary function test and were classified as having mild, moderate or severe asthma. Severe asthma endotypes were defined according to the percentage of Th2 and Th17 cells in the peripheral blood and by the type of inflammation. The percentage of Th2 and Th17 cells in the peripheral blood was determined by flow cytometry. Serum MBD2, eosinophilic cationic protein and myeloperoxidase were measured by enzyme-linked immunosorbent assay. Correlations of MBD2 expression with clinical parameters were evaluated using Spearman's rank correlation analysis. Results: Serum MBD2 levels were upregulated in patients with severe asthma compared to healthy controls and patients with mild to moderate asthma. MBD2 was also significantly increased in patients with Th17 severe asthma compared to patients with type 2 severe asthma. Furthermore, MBD2 was positively correlated with MPO and Th17 cells but negatively correlated with ECP and Th2 cells in patients with severe asthma. Conclusions: These findings suggest that serum MBD2 may be a potential new biomarker for identifying severe asthma, Th17 severe asthma and the type of airway inflammation. However, these findings are still preliminary and need to be further investigated. Frontiers Media S.A. 2021-10-05 /pmc/articles/PMC8527858/ /pubmed/34692717 http://dx.doi.org/10.3389/fmed.2021.693605 Text en Copyright © 2021 Chen, Yuan, He, Wasti, Duan, Jia, Li, Xiao, Zhang, Ma, Li, Liu, Zeng, Xiang, Zhang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Chen, Zhifeng Yuan, Yu He, Yi Wasti, Binaya Duan, Wentao Jia, Jingsi Li, Danhong Xiao, Bing Zhang, Dongshan Ma, Libing Li, Jianmin Liu, Yi Zeng, Qingping Xiang, Xudong Zhang, Xiufeng Liu, Shaokun MBD2 as a Potential Novel Biomarker for Identifying Severe Asthma With Different Endotypes |
title | MBD2 as a Potential Novel Biomarker for Identifying Severe Asthma With Different Endotypes |
title_full | MBD2 as a Potential Novel Biomarker for Identifying Severe Asthma With Different Endotypes |
title_fullStr | MBD2 as a Potential Novel Biomarker for Identifying Severe Asthma With Different Endotypes |
title_full_unstemmed | MBD2 as a Potential Novel Biomarker for Identifying Severe Asthma With Different Endotypes |
title_short | MBD2 as a Potential Novel Biomarker for Identifying Severe Asthma With Different Endotypes |
title_sort | mbd2 as a potential novel biomarker for identifying severe asthma with different endotypes |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527858/ https://www.ncbi.nlm.nih.gov/pubmed/34692717 http://dx.doi.org/10.3389/fmed.2021.693605 |
work_keys_str_mv | AT chenzhifeng mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT yuanyu mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT heyi mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT wastibinaya mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT duanwentao mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT jiajingsi mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT lidanhong mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT xiaobing mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT zhangdongshan mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT malibing mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT lijianmin mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT liuyi mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT zengqingping mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT xiangxudong mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT zhangxiufeng mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes AT liushaokun mbd2asapotentialnovelbiomarkerforidentifyingsevereasthmawithdifferentendotypes |