Cargando…
Spatial and temporal changes in the PGE2 EP2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2
OBJECTIVE(S): Prostaglandin E2 E-prostanoid 2 receptor (PGE2 EP2), downstream of cyclooxygenase-2 (COX-2), plays an important role in inflammatory responses, but there are some reports about synaptic functions of COX-2 and PGE2 EP2 in the hippocampus. MATERIALS AND METHODS: C57BL/6J mice were sacrif...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528246/ https://www.ncbi.nlm.nih.gov/pubmed/34712420 http://dx.doi.org/10.22038/ijbms.2021.56286.12556 |
_version_ | 1784586211514384384 |
---|---|
author | Jung, Hyo Young Kim, Woosuk Hahn, Kyu Ri Nam, Sung Min Yi, Sun Shin Kwon, Hyun Jung Kang, Min Soo Choi, Jung Hoon Kim, Dae Won Yoon, Yeo Sung Hwang, In Koo |
author_facet | Jung, Hyo Young Kim, Woosuk Hahn, Kyu Ri Nam, Sung Min Yi, Sun Shin Kwon, Hyun Jung Kang, Min Soo Choi, Jung Hoon Kim, Dae Won Yoon, Yeo Sung Hwang, In Koo |
author_sort | Jung, Hyo Young |
collection | PubMed |
description | OBJECTIVE(S): Prostaglandin E2 E-prostanoid 2 receptor (PGE2 EP2), downstream of cyclooxygenase-2 (COX-2), plays an important role in inflammatory responses, but there are some reports about synaptic functions of COX-2 and PGE2 EP2 in the hippocampus. MATERIALS AND METHODS: C57BL/6J mice were sacrificed at postnatal days (P) 1, 7, 14, 28, and 56 for immunohistochemical staining for EP2 and doublecortin as well as western blot for EP2. In addition, COX-2 knockout and its wild-type mice were euthanized for immunohistochemical staining for EP2. RESULTS: EP2 immunoreactivity was observed in the majority of the cells in the dentate gyrus at P1 and P7, while at P14, it was detected in the outer granule cell layer and was confined to its subgranular zone at P28 and P56. EP2 protein levels in the hippocampal homogenates were also highest at P7 and lowest at P56. EP2 immunoreactivity was partially colocalized, with doublecortin (DCX)-immunoreactive neuroblasts appearing in the mid-zone of the granule cell layer at P14 and in the subgranular zone of the dentate gyrus at P28. Co-localization of EP2 and DCX was significantly decreased in the dentate gyrus in the P28 group compared with that in the P14 group. In COX-2 knockout mice, EP2 immunoreactivity was significantly decreased in the hippocampal CA1 region (P=0.000165) and dentate gyrus (P=0.00898). CONCLUSION: EP2 decreases with age, which is expressed in DCX-immunoreactive neuroblasts in the dentate gyrus. This suggests that EP2 is closely linked to structural lamination and adult neurogenesis in the dentate gyrus. |
format | Online Article Text |
id | pubmed-8528246 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-85282462021-10-27 Spatial and temporal changes in the PGE2 EP2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2 Jung, Hyo Young Kim, Woosuk Hahn, Kyu Ri Nam, Sung Min Yi, Sun Shin Kwon, Hyun Jung Kang, Min Soo Choi, Jung Hoon Kim, Dae Won Yoon, Yeo Sung Hwang, In Koo Iran J Basic Med Sci Original Article OBJECTIVE(S): Prostaglandin E2 E-prostanoid 2 receptor (PGE2 EP2), downstream of cyclooxygenase-2 (COX-2), plays an important role in inflammatory responses, but there are some reports about synaptic functions of COX-2 and PGE2 EP2 in the hippocampus. MATERIALS AND METHODS: C57BL/6J mice were sacrificed at postnatal days (P) 1, 7, 14, 28, and 56 for immunohistochemical staining for EP2 and doublecortin as well as western blot for EP2. In addition, COX-2 knockout and its wild-type mice were euthanized for immunohistochemical staining for EP2. RESULTS: EP2 immunoreactivity was observed in the majority of the cells in the dentate gyrus at P1 and P7, while at P14, it was detected in the outer granule cell layer and was confined to its subgranular zone at P28 and P56. EP2 protein levels in the hippocampal homogenates were also highest at P7 and lowest at P56. EP2 immunoreactivity was partially colocalized, with doublecortin (DCX)-immunoreactive neuroblasts appearing in the mid-zone of the granule cell layer at P14 and in the subgranular zone of the dentate gyrus at P28. Co-localization of EP2 and DCX was significantly decreased in the dentate gyrus in the P28 group compared with that in the P14 group. In COX-2 knockout mice, EP2 immunoreactivity was significantly decreased in the hippocampal CA1 region (P=0.000165) and dentate gyrus (P=0.00898). CONCLUSION: EP2 decreases with age, which is expressed in DCX-immunoreactive neuroblasts in the dentate gyrus. This suggests that EP2 is closely linked to structural lamination and adult neurogenesis in the dentate gyrus. Mashhad University of Medical Sciences 2021-07 /pmc/articles/PMC8528246/ /pubmed/34712420 http://dx.doi.org/10.22038/ijbms.2021.56286.12556 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jung, Hyo Young Kim, Woosuk Hahn, Kyu Ri Nam, Sung Min Yi, Sun Shin Kwon, Hyun Jung Kang, Min Soo Choi, Jung Hoon Kim, Dae Won Yoon, Yeo Sung Hwang, In Koo Spatial and temporal changes in the PGE2 EP2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2 |
title | Spatial and temporal changes in the PGE2 EP2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2 |
title_full | Spatial and temporal changes in the PGE2 EP2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2 |
title_fullStr | Spatial and temporal changes in the PGE2 EP2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2 |
title_full_unstemmed | Spatial and temporal changes in the PGE2 EP2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2 |
title_short | Spatial and temporal changes in the PGE2 EP2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2 |
title_sort | spatial and temporal changes in the pge2 ep2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528246/ https://www.ncbi.nlm.nih.gov/pubmed/34712420 http://dx.doi.org/10.22038/ijbms.2021.56286.12556 |
work_keys_str_mv | AT junghyoyoung spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT kimwoosuk spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT hahnkyuri spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT namsungmin spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT yisunshin spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT kwonhyunjung spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT kangminsoo spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT choijunghoon spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT kimdaewon spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT yoonyeosung spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 AT hwanginkoo spatialandtemporalchangesinthepge2ep2receptorinmicehippocampiduringpostnataldevelopmentanditsrelationshipwithcyclooxygenase2 |