Cargando…

Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats

OBJECTIVE(S): Ischemia/reperfusion (I/R) is a leading cause of myocardial infarction (MI) injury, contributing to excess injury to cardiac tissues involved in inflammation, apoptosis, and oxidative stress. The present study was conducted to examine the effects of combined thymoquinone (TQ) with isch...

Descripción completa

Detalles Bibliográficos
Autores principales: Ran, Junchuan, Xu, Huanglin, Li, Wenyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528251/
https://www.ncbi.nlm.nih.gov/pubmed/34712418
http://dx.doi.org/10.22038/ijbms.2021.47670.10981
_version_ 1784586212798889984
author Ran, Junchuan
Xu, Huanglin
Li, Wenyuan
author_facet Ran, Junchuan
Xu, Huanglin
Li, Wenyuan
author_sort Ran, Junchuan
collection PubMed
description OBJECTIVE(S): Ischemia/reperfusion (I/R) is a leading cause of myocardial infarction (MI) injury, contributing to excess injury to cardiac tissues involved in inflammation, apoptosis, and oxidative stress. The present study was conducted to examine the effects of combined thymoquinone (TQ) with ischemic postconditioning (IPostC) therapy on apoptosis and inflammation due to I/R injury in diabetic rat hearts. MATERIALS AND METHODS: A single dose injection of streptozotocin (STZ; 60 mg/kg) was administered to thirty-two Wistar male rats to induce diabetes. Hearts were fixed on a Langendorff setting and exposed to a 30 min regional ischemia subsequently to 60 min reperfusion. IPostC was induced at the onset of reperfusion by 3 cycles of 30 sec R/I. ELISA, Western blotting assay, and TUNEL staining were applied to assess the cardioprotective effect of IPostC and TQ against I/R injury in diabetic and non-diabetic rats. RESULTS: Administration of TQ alone in non-diabetic isolated hearts significantly diminished CK-MB, TNF-α, IL-1β, and apoptosis and enhanced p-GSK-3β and Bcl-2 (P<0.05). Following administration of TQ, the cardioprotective effects of IPostC by elevating p-GSK-3β and Bcl-2 and alleviating apoptosis and inflammation were reestablished compared with non-IPostC diabetic hearts. CONCLUSION: These results provide substantial evidence that co-administration of TQ plus IPostC can exert cardioprotective effects on diabetic myocardium during I/R damage by attenuating the inflammatory response and apoptosis.
format Online
Article
Text
id pubmed-8528251
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Mashhad University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-85282512021-10-27 Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats Ran, Junchuan Xu, Huanglin Li, Wenyuan Iran J Basic Med Sci Original Article OBJECTIVE(S): Ischemia/reperfusion (I/R) is a leading cause of myocardial infarction (MI) injury, contributing to excess injury to cardiac tissues involved in inflammation, apoptosis, and oxidative stress. The present study was conducted to examine the effects of combined thymoquinone (TQ) with ischemic postconditioning (IPostC) therapy on apoptosis and inflammation due to I/R injury in diabetic rat hearts. MATERIALS AND METHODS: A single dose injection of streptozotocin (STZ; 60 mg/kg) was administered to thirty-two Wistar male rats to induce diabetes. Hearts were fixed on a Langendorff setting and exposed to a 30 min regional ischemia subsequently to 60 min reperfusion. IPostC was induced at the onset of reperfusion by 3 cycles of 30 sec R/I. ELISA, Western blotting assay, and TUNEL staining were applied to assess the cardioprotective effect of IPostC and TQ against I/R injury in diabetic and non-diabetic rats. RESULTS: Administration of TQ alone in non-diabetic isolated hearts significantly diminished CK-MB, TNF-α, IL-1β, and apoptosis and enhanced p-GSK-3β and Bcl-2 (P<0.05). Following administration of TQ, the cardioprotective effects of IPostC by elevating p-GSK-3β and Bcl-2 and alleviating apoptosis and inflammation were reestablished compared with non-IPostC diabetic hearts. CONCLUSION: These results provide substantial evidence that co-administration of TQ plus IPostC can exert cardioprotective effects on diabetic myocardium during I/R damage by attenuating the inflammatory response and apoptosis. Mashhad University of Medical Sciences 2021-07 /pmc/articles/PMC8528251/ /pubmed/34712418 http://dx.doi.org/10.22038/ijbms.2021.47670.10981 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ran, Junchuan
Xu, Huanglin
Li, Wenyuan
Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats
title Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats
title_full Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats
title_fullStr Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats
title_full_unstemmed Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats
title_short Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats
title_sort cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528251/
https://www.ncbi.nlm.nih.gov/pubmed/34712418
http://dx.doi.org/10.22038/ijbms.2021.47670.10981
work_keys_str_mv AT ranjunchuan cardioprotectiveeffectsofcoadministrationofthymoquinoneandischemicpostconditioningindiabeticrats
AT xuhuanglin cardioprotectiveeffectsofcoadministrationofthymoquinoneandischemicpostconditioningindiabeticrats
AT liwenyuan cardioprotectiveeffectsofcoadministrationofthymoquinoneandischemicpostconditioningindiabeticrats