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Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings

The General Organization of the Veterinary Services in Egypt has adopted a sheeppox vaccination policy to control lumpy skin disease (LSD) in cattle. Over the course of the last two years, recurrent outbreaks were reported, with animals showing severe clinical signs and consequentially higher fatali...

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Autores principales: Rouby, Sherin R., Safwat, Nesreen M., Hussein, Khaled H., Abdel- Ra’ouf, Aml M., Madkour, Bahaa S., Abdel-Moneim, Ahmed S., Hosein, Hosein I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528327/
https://www.ncbi.nlm.nih.gov/pubmed/34669748
http://dx.doi.org/10.1371/journal.pone.0258755
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author Rouby, Sherin R.
Safwat, Nesreen M.
Hussein, Khaled H.
Abdel- Ra’ouf, Aml M.
Madkour, Bahaa S.
Abdel-Moneim, Ahmed S.
Hosein, Hosein I.
author_facet Rouby, Sherin R.
Safwat, Nesreen M.
Hussein, Khaled H.
Abdel- Ra’ouf, Aml M.
Madkour, Bahaa S.
Abdel-Moneim, Ahmed S.
Hosein, Hosein I.
author_sort Rouby, Sherin R.
collection PubMed
description The General Organization of the Veterinary Services in Egypt has adopted a sheeppox vaccination policy to control lumpy skin disease (LSD) in cattle. Over the course of the last two years, recurrent outbreaks were reported, with animals showing severe clinical signs and consequentially higher fatalities than that of cases reported in previous LSD outbreaks. A total of 1050 cattle showing typical clinical signs suggestive of LSD were clinically and pathologically investigated during 2017–2018. Skin nodules were collected and lumpy skin disease virus (LSDV) was screened in collected skin samples using PCR for the RPO-30 gene. Furthermore, the entire P32 protein coding gene was sequenced. Histopathology and immunohistochemistry of the skin nodules were also conducted. The obtained results showed an overall mortality rate of 6.86%. LSDV was confirmed in all the examined nodules as evidenced by immunohistochemistry and positive PCR amplification of the RPO30 gene. Sequencing analysis of the P32 gene revealed a highly conserved nature and genetic stability of the LSDV. The results of the present study show that the current vaccination protocol was not effective for a multitude of reasons. These results also serve as evidence for a strong recommendation of an amendment of homologous vaccine use aside from a complete coverage of cattle populations in order to reduce the incidence of LSD among cattle population in Egypt.
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spelling pubmed-85283272021-10-21 Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings Rouby, Sherin R. Safwat, Nesreen M. Hussein, Khaled H. Abdel- Ra’ouf, Aml M. Madkour, Bahaa S. Abdel-Moneim, Ahmed S. Hosein, Hosein I. PLoS One Research Article The General Organization of the Veterinary Services in Egypt has adopted a sheeppox vaccination policy to control lumpy skin disease (LSD) in cattle. Over the course of the last two years, recurrent outbreaks were reported, with animals showing severe clinical signs and consequentially higher fatalities than that of cases reported in previous LSD outbreaks. A total of 1050 cattle showing typical clinical signs suggestive of LSD were clinically and pathologically investigated during 2017–2018. Skin nodules were collected and lumpy skin disease virus (LSDV) was screened in collected skin samples using PCR for the RPO-30 gene. Furthermore, the entire P32 protein coding gene was sequenced. Histopathology and immunohistochemistry of the skin nodules were also conducted. The obtained results showed an overall mortality rate of 6.86%. LSDV was confirmed in all the examined nodules as evidenced by immunohistochemistry and positive PCR amplification of the RPO30 gene. Sequencing analysis of the P32 gene revealed a highly conserved nature and genetic stability of the LSDV. The results of the present study show that the current vaccination protocol was not effective for a multitude of reasons. These results also serve as evidence for a strong recommendation of an amendment of homologous vaccine use aside from a complete coverage of cattle populations in order to reduce the incidence of LSD among cattle population in Egypt. Public Library of Science 2021-10-20 /pmc/articles/PMC8528327/ /pubmed/34669748 http://dx.doi.org/10.1371/journal.pone.0258755 Text en © 2021 Rouby et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rouby, Sherin R.
Safwat, Nesreen M.
Hussein, Khaled H.
Abdel- Ra’ouf, Aml M.
Madkour, Bahaa S.
Abdel-Moneim, Ahmed S.
Hosein, Hosein I.
Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings
title Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings
title_full Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings
title_fullStr Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings
title_full_unstemmed Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings
title_short Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings
title_sort lumpy skin disease outbreaks in egypt during 2017-2018 among sheeppox vaccinated cattle: epidemiological, pathological, and molecular findings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528327/
https://www.ncbi.nlm.nih.gov/pubmed/34669748
http://dx.doi.org/10.1371/journal.pone.0258755
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