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Development and Evaluation of the Wound Healing Effect of a Novel Topical Cream Formula Based on Ginkgo biloba Extract on Wounds in Diabetic Rats

Despite advances in diabetes care, impaired diabetic wound healing remains a significant clinical problem. The present study was aimed at developing a novel cream based on Ginkgo biloba extract and investigating its wound healing effect on full-thickness wounds in diabetic rats. The topical formulat...

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Autores principales: Bardaa, Sana, Makni, Khouloud, Boudaouara, Ons, Bardaa, Tarek, Ktari, Naourez, Hachicha, Selim, Ben Salah, Riadh, Kallel, Rim, Sahnoun, Zouheir, Boufi, Sami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528584/
https://www.ncbi.nlm.nih.gov/pubmed/34692837
http://dx.doi.org/10.1155/2021/6474706
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author Bardaa, Sana
Makni, Khouloud
Boudaouara, Ons
Bardaa, Tarek
Ktari, Naourez
Hachicha, Selim
Ben Salah, Riadh
Kallel, Rim
Sahnoun, Zouheir
Boufi, Sami
author_facet Bardaa, Sana
Makni, Khouloud
Boudaouara, Ons
Bardaa, Tarek
Ktari, Naourez
Hachicha, Selim
Ben Salah, Riadh
Kallel, Rim
Sahnoun, Zouheir
Boufi, Sami
author_sort Bardaa, Sana
collection PubMed
description Despite advances in diabetes care, impaired diabetic wound healing remains a significant clinical problem. The present study was aimed at developing a novel cream based on Ginkgo biloba extract and investigating its wound healing effect on full-thickness wounds in diabetic rats. The topical formulated oil-in-water emulsion-based cream contains Ginkgo biloba aqueous extract in an amount of about 1% to 5% as an active agent. The prepared formula was subjected to physicochemical assessment and pharmacotechnical characterization. Eighteen alloxan-induced diabetic rats completing full-thickness excisional skin wounds were randomly divided into three groups topically treated with either a normal saline (control group), the reference drug (“Cytol Centella cream®”), and cream based on the Ginkgo biloba extract. The response to treatment was assessed by macroscopic, qualitative, and quantitative histopathological analysis. The prepared formula showed good physicochemical properties. The rheological behavior of the prepared cream followed a non-Newtonian pseudoplastic pattern at different storage temperatures. The cream, which is a macroemulsion with uniform size distribution, remained stable for 6 months. Skin tolerance studies confirmed the compatibility of the cream with the skin. During the experimental trial, the cream based on the Ginkgo biloba-treated group showed significant improvements over the control and reference groups for both general wound appearance and healing dynamics. This increased rate of closure of wounds in diabetic rats was associated with increased collagen synthesis. Our findings showed that the cream could be a promising and innovative topical treatment with Ginkgo biloba extract for the management of acute diabetic wounds.
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spelling pubmed-85285842021-10-21 Development and Evaluation of the Wound Healing Effect of a Novel Topical Cream Formula Based on Ginkgo biloba Extract on Wounds in Diabetic Rats Bardaa, Sana Makni, Khouloud Boudaouara, Ons Bardaa, Tarek Ktari, Naourez Hachicha, Selim Ben Salah, Riadh Kallel, Rim Sahnoun, Zouheir Boufi, Sami Biomed Res Int Research Article Despite advances in diabetes care, impaired diabetic wound healing remains a significant clinical problem. The present study was aimed at developing a novel cream based on Ginkgo biloba extract and investigating its wound healing effect on full-thickness wounds in diabetic rats. The topical formulated oil-in-water emulsion-based cream contains Ginkgo biloba aqueous extract in an amount of about 1% to 5% as an active agent. The prepared formula was subjected to physicochemical assessment and pharmacotechnical characterization. Eighteen alloxan-induced diabetic rats completing full-thickness excisional skin wounds were randomly divided into three groups topically treated with either a normal saline (control group), the reference drug (“Cytol Centella cream®”), and cream based on the Ginkgo biloba extract. The response to treatment was assessed by macroscopic, qualitative, and quantitative histopathological analysis. The prepared formula showed good physicochemical properties. The rheological behavior of the prepared cream followed a non-Newtonian pseudoplastic pattern at different storage temperatures. The cream, which is a macroemulsion with uniform size distribution, remained stable for 6 months. Skin tolerance studies confirmed the compatibility of the cream with the skin. During the experimental trial, the cream based on the Ginkgo biloba-treated group showed significant improvements over the control and reference groups for both general wound appearance and healing dynamics. This increased rate of closure of wounds in diabetic rats was associated with increased collagen synthesis. Our findings showed that the cream could be a promising and innovative topical treatment with Ginkgo biloba extract for the management of acute diabetic wounds. Hindawi 2021-10-13 /pmc/articles/PMC8528584/ /pubmed/34692837 http://dx.doi.org/10.1155/2021/6474706 Text en Copyright © 2021 Sana Bardaa et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bardaa, Sana
Makni, Khouloud
Boudaouara, Ons
Bardaa, Tarek
Ktari, Naourez
Hachicha, Selim
Ben Salah, Riadh
Kallel, Rim
Sahnoun, Zouheir
Boufi, Sami
Development and Evaluation of the Wound Healing Effect of a Novel Topical Cream Formula Based on Ginkgo biloba Extract on Wounds in Diabetic Rats
title Development and Evaluation of the Wound Healing Effect of a Novel Topical Cream Formula Based on Ginkgo biloba Extract on Wounds in Diabetic Rats
title_full Development and Evaluation of the Wound Healing Effect of a Novel Topical Cream Formula Based on Ginkgo biloba Extract on Wounds in Diabetic Rats
title_fullStr Development and Evaluation of the Wound Healing Effect of a Novel Topical Cream Formula Based on Ginkgo biloba Extract on Wounds in Diabetic Rats
title_full_unstemmed Development and Evaluation of the Wound Healing Effect of a Novel Topical Cream Formula Based on Ginkgo biloba Extract on Wounds in Diabetic Rats
title_short Development and Evaluation of the Wound Healing Effect of a Novel Topical Cream Formula Based on Ginkgo biloba Extract on Wounds in Diabetic Rats
title_sort development and evaluation of the wound healing effect of a novel topical cream formula based on ginkgo biloba extract on wounds in diabetic rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528584/
https://www.ncbi.nlm.nih.gov/pubmed/34692837
http://dx.doi.org/10.1155/2021/6474706
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