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In-depth analysis of N(2)O fluxes in tropical forest soils of the Congo Basin combining isotope and functional gene analysis
Primary tropical forests generally exhibit large gaseous nitrogen (N) losses, occurring as nitric oxide (NO), nitrous oxide (N(2)O) or elemental nitrogen (N(2)). The release of N(2)O is of particular concern due to its high global warming potential and destruction of stratospheric ozone. Tropical fo...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528805/ https://www.ncbi.nlm.nih.gov/pubmed/34035444 http://dx.doi.org/10.1038/s41396-021-01004-x |
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author | Gallarotti, Nora Barthel, Matti Verhoeven, Elizabeth Pereira, Engil Isadora Pujol Bauters, Marijn Baumgartner, Simon Drake, Travis W. Boeckx, Pascal Mohn, Joachim Longepierre, Manon Mugula, John Kalume Makelele, Isaac Ahanamungu Ntaboba, Landry Cizungu Six, Johan |
author_facet | Gallarotti, Nora Barthel, Matti Verhoeven, Elizabeth Pereira, Engil Isadora Pujol Bauters, Marijn Baumgartner, Simon Drake, Travis W. Boeckx, Pascal Mohn, Joachim Longepierre, Manon Mugula, John Kalume Makelele, Isaac Ahanamungu Ntaboba, Landry Cizungu Six, Johan |
author_sort | Gallarotti, Nora |
collection | PubMed |
description | Primary tropical forests generally exhibit large gaseous nitrogen (N) losses, occurring as nitric oxide (NO), nitrous oxide (N(2)O) or elemental nitrogen (N(2)). The release of N(2)O is of particular concern due to its high global warming potential and destruction of stratospheric ozone. Tropical forest soils are predicted to be among the largest natural sources of N(2)O; however, despite being the world’s second-largest rainforest, measurements of gaseous N-losses from forest soils of the Congo Basin are scarce. In addition, long-term studies investigating N(2)O fluxes from different forest ecosystem types (lowland and montane forests) are scarce. In this study we show that fluxes measured in the Congo Basin were lower than fluxes measured in the Neotropics, and in the tropical forests of Australia and South East Asia. In addition, we show that despite different climatic conditions, average annual N(2)O fluxes in the Congo Basin’s lowland forests (0.97 ± 0.53 kg N ha(−1) year(−1)) were comparable to those in its montane forest (0.88 ± 0.97 kg N ha(−1) year(−1)). Measurements of soil pore air N(2)O isotope data at multiple depths suggests that a microbial reduction of N(2)O to N(2) within the soil may account for the observed low surface N(2)O fluxes and low soil pore N(2)O concentrations. The potential for microbial reduction is corroborated by a significant abundance and expression of the gene nosZ in soil samples from both study sites. Although isotopic and functional gene analyses indicate an enzymatic potential for complete denitrification, combined gaseous N-losses (N(2)O, N(2)) are unlikely to account for the missing N-sink in these forests. Other N-losses such as NO, N(2) via Feammox or hydrological particulate organic nitrogen export could play an important role in soils of the Congo Basin and should be the focus of future research. |
format | Online Article Text |
id | pubmed-8528805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85288052021-10-22 In-depth analysis of N(2)O fluxes in tropical forest soils of the Congo Basin combining isotope and functional gene analysis Gallarotti, Nora Barthel, Matti Verhoeven, Elizabeth Pereira, Engil Isadora Pujol Bauters, Marijn Baumgartner, Simon Drake, Travis W. Boeckx, Pascal Mohn, Joachim Longepierre, Manon Mugula, John Kalume Makelele, Isaac Ahanamungu Ntaboba, Landry Cizungu Six, Johan ISME J Article Primary tropical forests generally exhibit large gaseous nitrogen (N) losses, occurring as nitric oxide (NO), nitrous oxide (N(2)O) or elemental nitrogen (N(2)). The release of N(2)O is of particular concern due to its high global warming potential and destruction of stratospheric ozone. Tropical forest soils are predicted to be among the largest natural sources of N(2)O; however, despite being the world’s second-largest rainforest, measurements of gaseous N-losses from forest soils of the Congo Basin are scarce. In addition, long-term studies investigating N(2)O fluxes from different forest ecosystem types (lowland and montane forests) are scarce. In this study we show that fluxes measured in the Congo Basin were lower than fluxes measured in the Neotropics, and in the tropical forests of Australia and South East Asia. In addition, we show that despite different climatic conditions, average annual N(2)O fluxes in the Congo Basin’s lowland forests (0.97 ± 0.53 kg N ha(−1) year(−1)) were comparable to those in its montane forest (0.88 ± 0.97 kg N ha(−1) year(−1)). Measurements of soil pore air N(2)O isotope data at multiple depths suggests that a microbial reduction of N(2)O to N(2) within the soil may account for the observed low surface N(2)O fluxes and low soil pore N(2)O concentrations. The potential for microbial reduction is corroborated by a significant abundance and expression of the gene nosZ in soil samples from both study sites. Although isotopic and functional gene analyses indicate an enzymatic potential for complete denitrification, combined gaseous N-losses (N(2)O, N(2)) are unlikely to account for the missing N-sink in these forests. Other N-losses such as NO, N(2) via Feammox or hydrological particulate organic nitrogen export could play an important role in soils of the Congo Basin and should be the focus of future research. Nature Publishing Group UK 2021-05-25 2021-11 /pmc/articles/PMC8528805/ /pubmed/34035444 http://dx.doi.org/10.1038/s41396-021-01004-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Gallarotti, Nora Barthel, Matti Verhoeven, Elizabeth Pereira, Engil Isadora Pujol Bauters, Marijn Baumgartner, Simon Drake, Travis W. Boeckx, Pascal Mohn, Joachim Longepierre, Manon Mugula, John Kalume Makelele, Isaac Ahanamungu Ntaboba, Landry Cizungu Six, Johan In-depth analysis of N(2)O fluxes in tropical forest soils of the Congo Basin combining isotope and functional gene analysis |
title | In-depth analysis of N(2)O fluxes in tropical forest soils of the Congo Basin combining isotope and functional gene analysis |
title_full | In-depth analysis of N(2)O fluxes in tropical forest soils of the Congo Basin combining isotope and functional gene analysis |
title_fullStr | In-depth analysis of N(2)O fluxes in tropical forest soils of the Congo Basin combining isotope and functional gene analysis |
title_full_unstemmed | In-depth analysis of N(2)O fluxes in tropical forest soils of the Congo Basin combining isotope and functional gene analysis |
title_short | In-depth analysis of N(2)O fluxes in tropical forest soils of the Congo Basin combining isotope and functional gene analysis |
title_sort | in-depth analysis of n(2)o fluxes in tropical forest soils of the congo basin combining isotope and functional gene analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528805/ https://www.ncbi.nlm.nih.gov/pubmed/34035444 http://dx.doi.org/10.1038/s41396-021-01004-x |
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