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Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco

Homeodomain-leucine zippers (HD-Zip) are plant-specific transcription factors that participate in different plant development processes and differentially regulate metabolic processes. LoHDZ2 is an HD-ZipII subfamily transcription factor gene that we identified from a transcriptomic analysis of Lari...

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Autores principales: An, Peiqi, Wang, Chen, Cao, Qing, Zhao, Qingrong, Qin, Ruofan, Zhang, Lei, Zhang, Hanguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528859/
https://www.ncbi.nlm.nih.gov/pubmed/34671092
http://dx.doi.org/10.1038/s41598-021-99533-0
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author An, Peiqi
Wang, Chen
Cao, Qing
Zhao, Qingrong
Qin, Ruofan
Zhang, Lei
Zhang, Hanguo
author_facet An, Peiqi
Wang, Chen
Cao, Qing
Zhao, Qingrong
Qin, Ruofan
Zhang, Lei
Zhang, Hanguo
author_sort An, Peiqi
collection PubMed
description Homeodomain-leucine zippers (HD-Zip) are plant-specific transcription factors that participate in different plant development processes and differentially regulate metabolic processes. LoHDZ2 is an HD-ZipII subfamily transcription factor gene that we identified from a transcriptomic analysis of Larix olgensis. To understand its function, we built a LoHDZ2 expression vector and then inserted it into tobacco by genetic transformation. Transgenic plants were identified at the DNA and RNA levels. Phenotypic index analysis of transgenic tobacco showed dwarfed growth with larger leaves and earlier flowering than the wild type. LoHDZ2 was expressed differently after hormone treatment with IAA, MeJA and 2,4-D. The results suggested that LoHDZ2 may respond to hormones and be involved in regulating growth and metabolism. These results helped us better understand the function of LoHDZ2 and provided a candidate gene for Larix olgensis molecular breeding.
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spelling pubmed-85288592021-10-22 Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco An, Peiqi Wang, Chen Cao, Qing Zhao, Qingrong Qin, Ruofan Zhang, Lei Zhang, Hanguo Sci Rep Article Homeodomain-leucine zippers (HD-Zip) are plant-specific transcription factors that participate in different plant development processes and differentially regulate metabolic processes. LoHDZ2 is an HD-ZipII subfamily transcription factor gene that we identified from a transcriptomic analysis of Larix olgensis. To understand its function, we built a LoHDZ2 expression vector and then inserted it into tobacco by genetic transformation. Transgenic plants were identified at the DNA and RNA levels. Phenotypic index analysis of transgenic tobacco showed dwarfed growth with larger leaves and earlier flowering than the wild type. LoHDZ2 was expressed differently after hormone treatment with IAA, MeJA and 2,4-D. The results suggested that LoHDZ2 may respond to hormones and be involved in regulating growth and metabolism. These results helped us better understand the function of LoHDZ2 and provided a candidate gene for Larix olgensis molecular breeding. Nature Publishing Group UK 2021-10-20 /pmc/articles/PMC8528859/ /pubmed/34671092 http://dx.doi.org/10.1038/s41598-021-99533-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
An, Peiqi
Wang, Chen
Cao, Qing
Zhao, Qingrong
Qin, Ruofan
Zhang, Lei
Zhang, Hanguo
Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco
title Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco
title_full Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco
title_fullStr Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco
title_full_unstemmed Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco
title_short Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco
title_sort genetic transformation and growth index determination of the larix olgensis lohdz2 transcription factor gene in tobacco
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528859/
https://www.ncbi.nlm.nih.gov/pubmed/34671092
http://dx.doi.org/10.1038/s41598-021-99533-0
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