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Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm

BACKGROUND: Porcine vesicular disease is caused by the Seneca Valley virus (SVV), it is a novel Picornaviridae, which is prevalent in several countries. However, the pathogenicity of SVV on 5–6 week old pigs and the transmission routes of SVV remain unknown. METHODS: This research mainly focuses on...

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Autores principales: Zhang, Jinyong, Li, Chenghui, Meng, Yuan, Xie, Yubiao, Shi, Ning, Zhang, He, Yu, Chengdong, Nan, Fulong, Xie, Changzhan, Ha, Zhuo, Han, Jicheng, Li, Zhuoxin, Li, Qiuxuan, Wang, Peng, Gao, Xu, Jin, Ningyi, Lu, Huijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529370/
https://www.ncbi.nlm.nih.gov/pubmed/34674719
http://dx.doi.org/10.1186/s12985-021-01679-w
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author Zhang, Jinyong
Li, Chenghui
Meng, Yuan
Xie, Yubiao
Shi, Ning
Zhang, He
Yu, Chengdong
Nan, Fulong
Xie, Changzhan
Ha, Zhuo
Han, Jicheng
Li, Zhuoxin
Li, Qiuxuan
Wang, Peng
Gao, Xu
Jin, Ningyi
Lu, Huijun
author_facet Zhang, Jinyong
Li, Chenghui
Meng, Yuan
Xie, Yubiao
Shi, Ning
Zhang, He
Yu, Chengdong
Nan, Fulong
Xie, Changzhan
Ha, Zhuo
Han, Jicheng
Li, Zhuoxin
Li, Qiuxuan
Wang, Peng
Gao, Xu
Jin, Ningyi
Lu, Huijun
author_sort Zhang, Jinyong
collection PubMed
description BACKGROUND: Porcine vesicular disease is caused by the Seneca Valley virus (SVV), it is a novel Picornaviridae, which is prevalent in several countries. However, the pathogenicity of SVV on 5–6 week old pigs and the transmission routes of SVV remain unknown. METHODS: This research mainly focuses on the pathogenicity of the CH-GX-01-2019 strain and the possible vector of SVV. In this study, 5–6 week old pigs infected with SVV (CH-GX-01-2019) and its clinical symptoms (including rectal temperatures and other clinical symptoms) were monitored, qRT-PCR were used to detect the viremia and virus distribution. Neutralization antibody assay was set up during this research. Mosquitoes and Culicoides were collected from pigsties after pigs challenge with SVV, and SVV detection within mosquitoes and Culicoides was done via RT-PCR. RESULTS: The challenged pigs presented with low fevers and mild lethargy on 5–8 days post infection. The viremia lasted more than 14 days. SVV was detected in almost all tissues on the 14th day following the challenge, and it was significantly higher in the hoofs (vesicles) and lymph nodes in comparison with other tissues. Neutralizing antibodies were also detected and could persist for more than 28 days, in addition neutralizing antibody titers ranged from 1:128 to 1:512. Mosquitoes and Culicoides were collected from the pigsty environments following SVV infection. Although SVV was not detected in the mosquitoes, it was present in the Culicoides, however SVV could not be isolated from the positive Culicoides. CONCLUSIONS: Our work has enriched the knowledge relating to SVV pathogenicity and possible transmission routes, which may lay the foundation for further research into the prevention and control of this virus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-021-01679-w.
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spelling pubmed-85293702021-10-21 Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm Zhang, Jinyong Li, Chenghui Meng, Yuan Xie, Yubiao Shi, Ning Zhang, He Yu, Chengdong Nan, Fulong Xie, Changzhan Ha, Zhuo Han, Jicheng Li, Zhuoxin Li, Qiuxuan Wang, Peng Gao, Xu Jin, Ningyi Lu, Huijun Virol J Research BACKGROUND: Porcine vesicular disease is caused by the Seneca Valley virus (SVV), it is a novel Picornaviridae, which is prevalent in several countries. However, the pathogenicity of SVV on 5–6 week old pigs and the transmission routes of SVV remain unknown. METHODS: This research mainly focuses on the pathogenicity of the CH-GX-01-2019 strain and the possible vector of SVV. In this study, 5–6 week old pigs infected with SVV (CH-GX-01-2019) and its clinical symptoms (including rectal temperatures and other clinical symptoms) were monitored, qRT-PCR were used to detect the viremia and virus distribution. Neutralization antibody assay was set up during this research. Mosquitoes and Culicoides were collected from pigsties after pigs challenge with SVV, and SVV detection within mosquitoes and Culicoides was done via RT-PCR. RESULTS: The challenged pigs presented with low fevers and mild lethargy on 5–8 days post infection. The viremia lasted more than 14 days. SVV was detected in almost all tissues on the 14th day following the challenge, and it was significantly higher in the hoofs (vesicles) and lymph nodes in comparison with other tissues. Neutralizing antibodies were also detected and could persist for more than 28 days, in addition neutralizing antibody titers ranged from 1:128 to 1:512. Mosquitoes and Culicoides were collected from the pigsty environments following SVV infection. Although SVV was not detected in the mosquitoes, it was present in the Culicoides, however SVV could not be isolated from the positive Culicoides. CONCLUSIONS: Our work has enriched the knowledge relating to SVV pathogenicity and possible transmission routes, which may lay the foundation for further research into the prevention and control of this virus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-021-01679-w. BioMed Central 2021-10-21 /pmc/articles/PMC8529370/ /pubmed/34674719 http://dx.doi.org/10.1186/s12985-021-01679-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Jinyong
Li, Chenghui
Meng, Yuan
Xie, Yubiao
Shi, Ning
Zhang, He
Yu, Chengdong
Nan, Fulong
Xie, Changzhan
Ha, Zhuo
Han, Jicheng
Li, Zhuoxin
Li, Qiuxuan
Wang, Peng
Gao, Xu
Jin, Ningyi
Lu, Huijun
Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm
title Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm
title_full Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm
title_fullStr Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm
title_full_unstemmed Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm
title_short Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm
title_sort pathogenicity of seneca valley virus in pigs and detection in culicoides from an infected pig farm
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529370/
https://www.ncbi.nlm.nih.gov/pubmed/34674719
http://dx.doi.org/10.1186/s12985-021-01679-w
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