FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signalin...

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Detalles Bibliográficos
Autores principales: Di Franco, Simone, Zhang, Le, Gaggianesi, Miriam, Lo Iacono, Melania, Medema, Jan Paul, Stassi, Giorgio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529548/
https://www.ncbi.nlm.nih.gov/pubmed/34712995
http://dx.doi.org/10.1016/j.xpro.2021.100880
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author Di Franco, Simone
Zhang, Le
Gaggianesi, Miriam
Lo Iacono, Melania
Medema, Jan Paul
Stassi, Giorgio
author_facet Di Franco, Simone
Zhang, Le
Gaggianesi, Miriam
Lo Iacono, Melania
Medema, Jan Paul
Stassi, Giorgio
author_sort Di Franco, Simone
collection PubMed
description Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).
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spelling pubmed-85295482021-10-27 FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter Di Franco, Simone Zhang, Le Gaggianesi, Miriam Lo Iacono, Melania Medema, Jan Paul Stassi, Giorgio STAR Protoc Protocol Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021). Elsevier 2021-10-19 /pmc/articles/PMC8529548/ /pubmed/34712995 http://dx.doi.org/10.1016/j.xpro.2021.100880 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Di Franco, Simone
Zhang, Le
Gaggianesi, Miriam
Lo Iacono, Melania
Medema, Jan Paul
Stassi, Giorgio
FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter
title FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter
title_full FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter
title_fullStr FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter
title_full_unstemmed FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter
title_short FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter
title_sort facs-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a wnt/β-catenin signaling pathway reporter
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529548/
https://www.ncbi.nlm.nih.gov/pubmed/34712995
http://dx.doi.org/10.1016/j.xpro.2021.100880
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