FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signalin...

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Detalles Bibliográficos
Autores principales: Di Franco, Simone, Zhang, Le, Gaggianesi, Miriam, Lo Iacono, Melania, Medema, Jan Paul, Stassi, Giorgio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529548/
https://www.ncbi.nlm.nih.gov/pubmed/34712995
http://dx.doi.org/10.1016/j.xpro.2021.100880

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