Co-staining human PBMCs with fluorescent antibodies and antibody-oligonucleotide conjugates for cell sorting prior to single-cell CITE-Seq

The dual interrogation of the transcriptome and proteome with single-cell resolution provides exquisite insights into immune mechanisms in health and disease. Here, we describe a cutting-edge protocol wherein we combine Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-Seq), a tec...

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Detalles Bibliográficos
Autores principales: Shi, Xiaoshan, Baracho, Gisele V., Lomas, Woodrow E., Widmann, Stephanie J., Tyznik, Aaron J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529558/
https://www.ncbi.nlm.nih.gov/pubmed/34712996
http://dx.doi.org/10.1016/j.xpro.2021.100893

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