Visualizing Teixobactin Supramolecular Assemblies and Cell Wall Damage in B. Subtilis Using CryoEM

[Image: see text] The antibiotic teixobactin targets bacterial cell walls. Previous research has proposed that the active form of teixobactin is a nano-/micron-sized supramolecular assembly. Here, we use cryogenic transmission electron microscopy to show that at 1 mg/mL, teixobactin forms sheet-like...

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Autores principales: Hurst, Paul Joshua, Morris, Michael A., Graham, Annissa A., Nowick, James S., Patterson, Joseph P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529686/
https://www.ncbi.nlm.nih.gov/pubmed/34693162
http://dx.doi.org/10.1021/acsomega.1c04331
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author Hurst, Paul Joshua
Morris, Michael A.
Graham, Annissa A.
Nowick, James S.
Patterson, Joseph P.
author_facet Hurst, Paul Joshua
Morris, Michael A.
Graham, Annissa A.
Nowick, James S.
Patterson, Joseph P.
author_sort Hurst, Paul Joshua
collection PubMed
description [Image: see text] The antibiotic teixobactin targets bacterial cell walls. Previous research has proposed that the active form of teixobactin is a nano-/micron-sized supramolecular assembly. Here, we use cryogenic transmission electron microscopy to show that at 1 mg/mL, teixobactin forms sheet-like assemblies that selectively act upon the cell wall. At 4 μg/mL, teixobactin is active, and aggregates are formed either transiently or sparingly at the cell surface.
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spelling pubmed-85296862021-10-22 Visualizing Teixobactin Supramolecular Assemblies and Cell Wall Damage in B. Subtilis Using CryoEM Hurst, Paul Joshua Morris, Michael A. Graham, Annissa A. Nowick, James S. Patterson, Joseph P. ACS Omega [Image: see text] The antibiotic teixobactin targets bacterial cell walls. Previous research has proposed that the active form of teixobactin is a nano-/micron-sized supramolecular assembly. Here, we use cryogenic transmission electron microscopy to show that at 1 mg/mL, teixobactin forms sheet-like assemblies that selectively act upon the cell wall. At 4 μg/mL, teixobactin is active, and aggregates are formed either transiently or sparingly at the cell surface. American Chemical Society 2021-10-07 /pmc/articles/PMC8529686/ /pubmed/34693162 http://dx.doi.org/10.1021/acsomega.1c04331 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Hurst, Paul Joshua
Morris, Michael A.
Graham, Annissa A.
Nowick, James S.
Patterson, Joseph P.
Visualizing Teixobactin Supramolecular Assemblies and Cell Wall Damage in B. Subtilis Using CryoEM
title Visualizing Teixobactin Supramolecular Assemblies and Cell Wall Damage in B. Subtilis Using CryoEM
title_full Visualizing Teixobactin Supramolecular Assemblies and Cell Wall Damage in B. Subtilis Using CryoEM
title_fullStr Visualizing Teixobactin Supramolecular Assemblies and Cell Wall Damage in B. Subtilis Using CryoEM
title_full_unstemmed Visualizing Teixobactin Supramolecular Assemblies and Cell Wall Damage in B. Subtilis Using CryoEM
title_short Visualizing Teixobactin Supramolecular Assemblies and Cell Wall Damage in B. Subtilis Using CryoEM
title_sort visualizing teixobactin supramolecular assemblies and cell wall damage in b. subtilis using cryoem
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529686/
https://www.ncbi.nlm.nih.gov/pubmed/34693162
http://dx.doi.org/10.1021/acsomega.1c04331
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