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Gastric bypass prevents diabetes in genetically modified mice and chemically induced diabetic mice

Obese subjects have increase probabilities of developing type 2 diabetes (T2D). In this study, we sought to determine whether gastric bypass prevents the progression of prediabetes to overt diabetes in genetically modified mice and chemically induced diabetic mice. Roux-en-Y gastric bypass (RYGB) wa...

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Autores principales: Zhu, Chenyu, Xu, Rui, Li, Yuxin, Andrade, Michael, Yin, Deng Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530305/
https://www.ncbi.nlm.nih.gov/pubmed/34673835
http://dx.doi.org/10.1371/journal.pone.0258942
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author Zhu, Chenyu
Xu, Rui
Li, Yuxin
Andrade, Michael
Yin, Deng Ping
author_facet Zhu, Chenyu
Xu, Rui
Li, Yuxin
Andrade, Michael
Yin, Deng Ping
author_sort Zhu, Chenyu
collection PubMed
description Obese subjects have increase probabilities of developing type 2 diabetes (T2D). In this study, we sought to determine whether gastric bypass prevents the progression of prediabetes to overt diabetes in genetically modified mice and chemically induced diabetic mice. Roux-en-Y gastric bypass (RYGB) was performed in C57BL/KsJ-db/db null (BKS-db/db,) mice, high-fat diet (HFD)-fed NONcNZO10/LtJ (NZO) mice, C57BL/6 db/db null (B6-db/db) mice and streptozotocin (STZ)-induced diabetic mice. Food consumption, body weight, fat mass, fast blood glucose level, circulating insulin and adiponectin and glucose tolerance test were analyzed. The liver and pancreatic tissues were subjected to H&E and immunohistochemistry staining and islet cells to flow cytometry for apoptotic analysis. RYGB resulted in sustained normoglycemia and improved glucose tolerance in young prediabetic BKS-db/db mice (at the age of 6 weeks with hyperglycemia and normal insulinemia) and HFD-fed NZO and B6-db/db mice. Remarkably, RYGB improved liver steatosis, preserved the pancreatic β-cells and reduced β-cell apoptosis with increases in circulating insulin and adiponectin in young prediabetic BKS-db/db mice. However, RYGB neither reversed hyperglycemia in adult diabetic BKS-db/db mice (12 weeks old) nor attenuated hyperglycemia in STZ-induced diabetic mice. These results demonstrate that gastric bypass improves hyperglycemia in genetically modified prediabetic mice; however, it should be performed prior to β-cells exhaustion.
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spelling pubmed-85303052021-10-22 Gastric bypass prevents diabetes in genetically modified mice and chemically induced diabetic mice Zhu, Chenyu Xu, Rui Li, Yuxin Andrade, Michael Yin, Deng Ping PLoS One Research Article Obese subjects have increase probabilities of developing type 2 diabetes (T2D). In this study, we sought to determine whether gastric bypass prevents the progression of prediabetes to overt diabetes in genetically modified mice and chemically induced diabetic mice. Roux-en-Y gastric bypass (RYGB) was performed in C57BL/KsJ-db/db null (BKS-db/db,) mice, high-fat diet (HFD)-fed NONcNZO10/LtJ (NZO) mice, C57BL/6 db/db null (B6-db/db) mice and streptozotocin (STZ)-induced diabetic mice. Food consumption, body weight, fat mass, fast blood glucose level, circulating insulin and adiponectin and glucose tolerance test were analyzed. The liver and pancreatic tissues were subjected to H&E and immunohistochemistry staining and islet cells to flow cytometry for apoptotic analysis. RYGB resulted in sustained normoglycemia and improved glucose tolerance in young prediabetic BKS-db/db mice (at the age of 6 weeks with hyperglycemia and normal insulinemia) and HFD-fed NZO and B6-db/db mice. Remarkably, RYGB improved liver steatosis, preserved the pancreatic β-cells and reduced β-cell apoptosis with increases in circulating insulin and adiponectin in young prediabetic BKS-db/db mice. However, RYGB neither reversed hyperglycemia in adult diabetic BKS-db/db mice (12 weeks old) nor attenuated hyperglycemia in STZ-induced diabetic mice. These results demonstrate that gastric bypass improves hyperglycemia in genetically modified prediabetic mice; however, it should be performed prior to β-cells exhaustion. Public Library of Science 2021-10-21 /pmc/articles/PMC8530305/ /pubmed/34673835 http://dx.doi.org/10.1371/journal.pone.0258942 Text en © 2021 Zhu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhu, Chenyu
Xu, Rui
Li, Yuxin
Andrade, Michael
Yin, Deng Ping
Gastric bypass prevents diabetes in genetically modified mice and chemically induced diabetic mice
title Gastric bypass prevents diabetes in genetically modified mice and chemically induced diabetic mice
title_full Gastric bypass prevents diabetes in genetically modified mice and chemically induced diabetic mice
title_fullStr Gastric bypass prevents diabetes in genetically modified mice and chemically induced diabetic mice
title_full_unstemmed Gastric bypass prevents diabetes in genetically modified mice and chemically induced diabetic mice
title_short Gastric bypass prevents diabetes in genetically modified mice and chemically induced diabetic mice
title_sort gastric bypass prevents diabetes in genetically modified mice and chemically induced diabetic mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530305/
https://www.ncbi.nlm.nih.gov/pubmed/34673835
http://dx.doi.org/10.1371/journal.pone.0258942
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