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A dual prokaryotic (E. coli) expression system (pdMAX)

In this study, we introduced an efficient subcloning and expression system with two inducible prokaryotic expression promoters, arabinose and lac, in a single plasmid in Escherichia coli. The arabinose promoter unit allows for the expression of a FLAG-tagged protein, while the isopropyl-β-D-thiogala...

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Detalles Bibliográficos
Autores principales: Murakami, Manabu, Murakami, Agnieszka M., Itagaki, Shirou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530331/
https://www.ncbi.nlm.nih.gov/pubmed/34673793
http://dx.doi.org/10.1371/journal.pone.0258553

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