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A dual prokaryotic (E. coli) expression system (pdMAX)
In this study, we introduced an efficient subcloning and expression system with two inducible prokaryotic expression promoters, arabinose and lac, in a single plasmid in Escherichia coli. The arabinose promoter unit allows for the expression of a FLAG-tagged protein, while the isopropyl-β-D-thiogala...
Autores principales: | Murakami, Manabu, Murakami, Agnieszka M., Itagaki, Shirou |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530331/ https://www.ncbi.nlm.nih.gov/pubmed/34673793 http://dx.doi.org/10.1371/journal.pone.0258553 |
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