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Metabolic gene regulation by Drosophila GATA transcription factor Grain

Nutrient-dependent gene regulation critically contributes to homeostatic control of animal physiology in changing nutrient landscape. In Drosophila, dietary sugars activate transcription factors (TFs), such as Mondo-Mlx, Sugarbabe and Cabut, which control metabolic gene expression to mediate physiol...

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Autores principales: Kokki, Krista, Lamichane, Nicole, Nieminen, Anni I., Ruhanen, Hanna, Morikka, Jack, Robciuc, Marius, Rovenko, Bohdana M., Havula, Essi, Käkelä, Reijo, Hietakangas, Ville
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530363/
https://www.ncbi.nlm.nih.gov/pubmed/34634038
http://dx.doi.org/10.1371/journal.pgen.1009855
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author Kokki, Krista
Lamichane, Nicole
Nieminen, Anni I.
Ruhanen, Hanna
Morikka, Jack
Robciuc, Marius
Rovenko, Bohdana M.
Havula, Essi
Käkelä, Reijo
Hietakangas, Ville
author_facet Kokki, Krista
Lamichane, Nicole
Nieminen, Anni I.
Ruhanen, Hanna
Morikka, Jack
Robciuc, Marius
Rovenko, Bohdana M.
Havula, Essi
Käkelä, Reijo
Hietakangas, Ville
author_sort Kokki, Krista
collection PubMed
description Nutrient-dependent gene regulation critically contributes to homeostatic control of animal physiology in changing nutrient landscape. In Drosophila, dietary sugars activate transcription factors (TFs), such as Mondo-Mlx, Sugarbabe and Cabut, which control metabolic gene expression to mediate physiological adaptation to high sugar diet. TFs that correspondingly control sugar responsive metabolic genes under conditions of low dietary sugar remain, however, poorly understood. Here we identify a role for Drosophila GATA TF Grain in metabolic gene regulation under both low and high sugar conditions. De novo motif prediction uncovered a significant over-representation of GATA-like motifs on the promoters of sugar-activated genes in Drosophila larvae, which are regulated by Grain, the fly ortholog of GATA1/2/3 subfamily. grain expression is activated by sugar in Mondo-Mlx-dependent manner and it contributes to sugar-responsive gene expression in the fat body. On the other hand, grain displays strong constitutive expression in the anterior midgut, where it drives lipogenic gene expression also under low sugar conditions. Consistently with these differential tissue-specific roles, Grain deficient larvae display delayed development on high sugar diet, while showing deregulated central carbon and lipid metabolism primarily on low sugar diet. Collectively, our study provides evidence for the role of a metazoan GATA transcription factor in nutrient-responsive metabolic gene regulation in vivo.
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spelling pubmed-85303632021-10-22 Metabolic gene regulation by Drosophila GATA transcription factor Grain Kokki, Krista Lamichane, Nicole Nieminen, Anni I. Ruhanen, Hanna Morikka, Jack Robciuc, Marius Rovenko, Bohdana M. Havula, Essi Käkelä, Reijo Hietakangas, Ville PLoS Genet Research Article Nutrient-dependent gene regulation critically contributes to homeostatic control of animal physiology in changing nutrient landscape. In Drosophila, dietary sugars activate transcription factors (TFs), such as Mondo-Mlx, Sugarbabe and Cabut, which control metabolic gene expression to mediate physiological adaptation to high sugar diet. TFs that correspondingly control sugar responsive metabolic genes under conditions of low dietary sugar remain, however, poorly understood. Here we identify a role for Drosophila GATA TF Grain in metabolic gene regulation under both low and high sugar conditions. De novo motif prediction uncovered a significant over-representation of GATA-like motifs on the promoters of sugar-activated genes in Drosophila larvae, which are regulated by Grain, the fly ortholog of GATA1/2/3 subfamily. grain expression is activated by sugar in Mondo-Mlx-dependent manner and it contributes to sugar-responsive gene expression in the fat body. On the other hand, grain displays strong constitutive expression in the anterior midgut, where it drives lipogenic gene expression also under low sugar conditions. Consistently with these differential tissue-specific roles, Grain deficient larvae display delayed development on high sugar diet, while showing deregulated central carbon and lipid metabolism primarily on low sugar diet. Collectively, our study provides evidence for the role of a metazoan GATA transcription factor in nutrient-responsive metabolic gene regulation in vivo. Public Library of Science 2021-10-11 /pmc/articles/PMC8530363/ /pubmed/34634038 http://dx.doi.org/10.1371/journal.pgen.1009855 Text en © 2021 Kokki et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kokki, Krista
Lamichane, Nicole
Nieminen, Anni I.
Ruhanen, Hanna
Morikka, Jack
Robciuc, Marius
Rovenko, Bohdana M.
Havula, Essi
Käkelä, Reijo
Hietakangas, Ville
Metabolic gene regulation by Drosophila GATA transcription factor Grain
title Metabolic gene regulation by Drosophila GATA transcription factor Grain
title_full Metabolic gene regulation by Drosophila GATA transcription factor Grain
title_fullStr Metabolic gene regulation by Drosophila GATA transcription factor Grain
title_full_unstemmed Metabolic gene regulation by Drosophila GATA transcription factor Grain
title_short Metabolic gene regulation by Drosophila GATA transcription factor Grain
title_sort metabolic gene regulation by drosophila gata transcription factor grain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530363/
https://www.ncbi.nlm.nih.gov/pubmed/34634038
http://dx.doi.org/10.1371/journal.pgen.1009855
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