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Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice

The total flavone of Abelmoschus manihot (TFA), a compound extracted from the flowers of Abelmoschus manihot (L.) Medic, has been widely used for the treatment of Crohn's disease, chronic glomerulonephritis and other diseases. The aim of this study was to investigate the effect of TFA on the gu...

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Autores principales: Bu, Fan, Ding, Yang, Chen, Tuo, Wang, Qiong, Wang, Rong, Zhou, Jin-yong, Jiang, Feng, Zhang, Dan, Xu, Minmin, Shi, Guoping, Chen, Yugen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531128/
https://www.ncbi.nlm.nih.gov/pubmed/34675239
http://dx.doi.org/10.1038/s41598-021-00070-7
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author Bu, Fan
Ding, Yang
Chen, Tuo
Wang, Qiong
Wang, Rong
Zhou, Jin-yong
Jiang, Feng
Zhang, Dan
Xu, Minmin
Shi, Guoping
Chen, Yugen
author_facet Bu, Fan
Ding, Yang
Chen, Tuo
Wang, Qiong
Wang, Rong
Zhou, Jin-yong
Jiang, Feng
Zhang, Dan
Xu, Minmin
Shi, Guoping
Chen, Yugen
author_sort Bu, Fan
collection PubMed
description The total flavone of Abelmoschus manihot (TFA), a compound extracted from the flowers of Abelmoschus manihot (L.) Medic, has been widely used for the treatment of Crohn's disease, chronic glomerulonephritis and other diseases. The aim of this study was to investigate the effect of TFA on the gut microbiota and intestinal barrier in dextran sulfate sodium (DSS)-induced experimental colitis. C57BL/6J mice were treated with 2.5% DSS in drinking water to induce colitis. Mice were orally administered TFA (62.5 mg/kg, 125 mg/kg) or prednisone acetate (PAT, 2.5 mg/kg) once daily for 7 days. Biological samples were collected for analysis of inflammatory cytokines, gut microbiota and intestinal barrier integrity. TFA-H (125 mg/kg) markedly attenuated DSS-induced colon shortening and histological injury in experimental colitis. The therapeutic effect was similar to that of PAT administration. TFA-H notably modulated the dysbiosis of gut microbiota induced by DSS and greatly enriched Akkermansia muciniphila (A. muciniphila). Moreover, TFA-H remarkably ameliorated the colonic inflammatory response and intestinal epithelial barrier dysfunction. Interestingly, TFA directly promotes the growth of A. muciniphila in vitro. Taken together, the results revealed for the first time that TFA, as a prebiotic of A. muciniphila, improved DSS-induced experimental colitis, at least partly by modulating the gut microflora profile to maintain colonic integrity and inhibit the inflammatory response.
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spelling pubmed-85311282021-10-22 Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice Bu, Fan Ding, Yang Chen, Tuo Wang, Qiong Wang, Rong Zhou, Jin-yong Jiang, Feng Zhang, Dan Xu, Minmin Shi, Guoping Chen, Yugen Sci Rep Article The total flavone of Abelmoschus manihot (TFA), a compound extracted from the flowers of Abelmoschus manihot (L.) Medic, has been widely used for the treatment of Crohn's disease, chronic glomerulonephritis and other diseases. The aim of this study was to investigate the effect of TFA on the gut microbiota and intestinal barrier in dextran sulfate sodium (DSS)-induced experimental colitis. C57BL/6J mice were treated with 2.5% DSS in drinking water to induce colitis. Mice were orally administered TFA (62.5 mg/kg, 125 mg/kg) or prednisone acetate (PAT, 2.5 mg/kg) once daily for 7 days. Biological samples were collected for analysis of inflammatory cytokines, gut microbiota and intestinal barrier integrity. TFA-H (125 mg/kg) markedly attenuated DSS-induced colon shortening and histological injury in experimental colitis. The therapeutic effect was similar to that of PAT administration. TFA-H notably modulated the dysbiosis of gut microbiota induced by DSS and greatly enriched Akkermansia muciniphila (A. muciniphila). Moreover, TFA-H remarkably ameliorated the colonic inflammatory response and intestinal epithelial barrier dysfunction. Interestingly, TFA directly promotes the growth of A. muciniphila in vitro. Taken together, the results revealed for the first time that TFA, as a prebiotic of A. muciniphila, improved DSS-induced experimental colitis, at least partly by modulating the gut microflora profile to maintain colonic integrity and inhibit the inflammatory response. Nature Publishing Group UK 2021-10-21 /pmc/articles/PMC8531128/ /pubmed/34675239 http://dx.doi.org/10.1038/s41598-021-00070-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Bu, Fan
Ding, Yang
Chen, Tuo
Wang, Qiong
Wang, Rong
Zhou, Jin-yong
Jiang, Feng
Zhang, Dan
Xu, Minmin
Shi, Guoping
Chen, Yugen
Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice
title Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice
title_full Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice
title_fullStr Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice
title_full_unstemmed Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice
title_short Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice
title_sort total flavone of abelmoschus manihot improves colitis by promoting the growth of akkermansia in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531128/
https://www.ncbi.nlm.nih.gov/pubmed/34675239
http://dx.doi.org/10.1038/s41598-021-00070-7
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