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FDX1 can Impact the Prognosis and Mediate the Metabolism of Lung Adenocarcinoma

Background: Lung cancer has emerged as one of the most common cancers in recent years. The mitochondrial electron transport chain (ETC) is closely connected with metabolic pathways and inflammatory response. However, the influence of ETC-associated genes on the tumor immune response and the pathogen...

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Autores principales: Zhang, Zeyu, Ma, Yarui, Guo, Xiaolei, Du, Yingxi, Zhu, Qing, Wang, Xiaobing, Duan, Changzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531531/
https://www.ncbi.nlm.nih.gov/pubmed/34690780
http://dx.doi.org/10.3389/fphar.2021.749134
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author Zhang, Zeyu
Ma, Yarui
Guo, Xiaolei
Du, Yingxi
Zhu, Qing
Wang, Xiaobing
Duan, Changzhu
author_facet Zhang, Zeyu
Ma, Yarui
Guo, Xiaolei
Du, Yingxi
Zhu, Qing
Wang, Xiaobing
Duan, Changzhu
author_sort Zhang, Zeyu
collection PubMed
description Background: Lung cancer has emerged as one of the most common cancers in recent years. The mitochondrial electron transport chain (ETC) is closely connected with metabolic pathways and inflammatory response. However, the influence of ETC-associated genes on the tumor immune response and the pathogenesis of lung cancer is not clear and needs further exploration. Methods: The RNA-sequencing transcriptome and clinical characteristic data of LUAD were downloaded from the Cancer Genome Atlas (TCGA) database. The LASSO algorithm was used to build the risk signature, and the prediction model was evaluated by the survival analysis and receiver operating characteristic curve. We explored the function of FDX1 through flow cytometry, molecular biological methods, and liquid chromatography–tandem mass spectrometry/mass spectrometry (LC–MS/MS). Results: 12 genes (FDX1, FDX2, LOXL2, ASPH, GLRX2, ALDH2, CYCS, AKR1A1, MAOB, RDH16, CYBB, and CYB5A) were selected to build the risk signature, and the risk score was calculated with the coefficients from the LASSO algorithm. The 1-year, 3-year, and 5-year area under the curve (AUC) of ROC curves of the dataset were 0.7, 0.674, and 0.692, respectively. Univariate Cox analysis and multivariate Cox regression analysis indicated that the risk signature is an independent risk factor for LUAD patients. Among these genes, we focused on the FDX1 gene, and we found that knockdown of FDX1 neither inhibited tumor cell growth nor did it induce apoptosis or abnormal cell cycle distribution. But FDX1 could promote the ATP production. Furthermore, our study showed that FDX1 was closely related to the glucose metabolism, fatty acid oxidation, and amino acid metabolism. Conclusion: Collectively, this study provides new clues about carcinogenesis induced by ETC-associated genes in LUAD and paves the way for finding potential targets of LUAD.
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spelling pubmed-85315312021-10-23 FDX1 can Impact the Prognosis and Mediate the Metabolism of Lung Adenocarcinoma Zhang, Zeyu Ma, Yarui Guo, Xiaolei Du, Yingxi Zhu, Qing Wang, Xiaobing Duan, Changzhu Front Pharmacol Pharmacology Background: Lung cancer has emerged as one of the most common cancers in recent years. The mitochondrial electron transport chain (ETC) is closely connected with metabolic pathways and inflammatory response. However, the influence of ETC-associated genes on the tumor immune response and the pathogenesis of lung cancer is not clear and needs further exploration. Methods: The RNA-sequencing transcriptome and clinical characteristic data of LUAD were downloaded from the Cancer Genome Atlas (TCGA) database. The LASSO algorithm was used to build the risk signature, and the prediction model was evaluated by the survival analysis and receiver operating characteristic curve. We explored the function of FDX1 through flow cytometry, molecular biological methods, and liquid chromatography–tandem mass spectrometry/mass spectrometry (LC–MS/MS). Results: 12 genes (FDX1, FDX2, LOXL2, ASPH, GLRX2, ALDH2, CYCS, AKR1A1, MAOB, RDH16, CYBB, and CYB5A) were selected to build the risk signature, and the risk score was calculated with the coefficients from the LASSO algorithm. The 1-year, 3-year, and 5-year area under the curve (AUC) of ROC curves of the dataset were 0.7, 0.674, and 0.692, respectively. Univariate Cox analysis and multivariate Cox regression analysis indicated that the risk signature is an independent risk factor for LUAD patients. Among these genes, we focused on the FDX1 gene, and we found that knockdown of FDX1 neither inhibited tumor cell growth nor did it induce apoptosis or abnormal cell cycle distribution. But FDX1 could promote the ATP production. Furthermore, our study showed that FDX1 was closely related to the glucose metabolism, fatty acid oxidation, and amino acid metabolism. Conclusion: Collectively, this study provides new clues about carcinogenesis induced by ETC-associated genes in LUAD and paves the way for finding potential targets of LUAD. Frontiers Media S.A. 2021-10-08 /pmc/articles/PMC8531531/ /pubmed/34690780 http://dx.doi.org/10.3389/fphar.2021.749134 Text en Copyright © 2021 Zhang, Ma, Guo, Du, Zhu, Wang and Duan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zhang, Zeyu
Ma, Yarui
Guo, Xiaolei
Du, Yingxi
Zhu, Qing
Wang, Xiaobing
Duan, Changzhu
FDX1 can Impact the Prognosis and Mediate the Metabolism of Lung Adenocarcinoma
title FDX1 can Impact the Prognosis and Mediate the Metabolism of Lung Adenocarcinoma
title_full FDX1 can Impact the Prognosis and Mediate the Metabolism of Lung Adenocarcinoma
title_fullStr FDX1 can Impact the Prognosis and Mediate the Metabolism of Lung Adenocarcinoma
title_full_unstemmed FDX1 can Impact the Prognosis and Mediate the Metabolism of Lung Adenocarcinoma
title_short FDX1 can Impact the Prognosis and Mediate the Metabolism of Lung Adenocarcinoma
title_sort fdx1 can impact the prognosis and mediate the metabolism of lung adenocarcinoma
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531531/
https://www.ncbi.nlm.nih.gov/pubmed/34690780
http://dx.doi.org/10.3389/fphar.2021.749134
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