A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system

CRISPR/Cas9 can be used as an experimental tool to inactivate genes in cells. However, a CRISPR-targeted cell population will not show a uniform genotype of the targeted gene. Instead, a mix of genotypes is generated - from wild type to different forms of insertions and deletions. Such mixed genotyp...

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Detalles Bibliográficos
Autores principales: Shen, Yunbing, Jiang, Long, Iyer, Vaishnavi Srinivasan, Raposo, Bruno, Dubnovitsky, Anatoly, Boddul, Sanjaykumar V., Kasza, Zsolt, Wermeling, Fredrik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531760/
https://www.ncbi.nlm.nih.gov/pubmed/34745454
http://dx.doi.org/10.1016/j.csbj.2021.09.020

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531760/
https://www.ncbi.nlm.nih.gov/pubmed/34745454
http://dx.doi.org/10.1016/j.csbj.2021.09.020

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