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Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism

Toxoplasmosis is a global threat with significant zoonotic concern. The present in silico study was aimed at determination of bioinformatics features and immunogenic epitopes of a tyrosine-rich oocyst wall protein (TrOWP) of Toxoplasma gondii. After retrieving the amino acid sequence from UniProt da...

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Autores principales: Asghari, Ali, Majidiani, Hamidreza, Nemati, Taher, Fatollahzadeh, Mohammad, Shams, Morteza, Naserifar, Razi, Kordi, Bahareh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531782/
https://www.ncbi.nlm.nih.gov/pubmed/34692826
http://dx.doi.org/10.1155/2021/1315618
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author Asghari, Ali
Majidiani, Hamidreza
Nemati, Taher
Fatollahzadeh, Mohammad
Shams, Morteza
Naserifar, Razi
Kordi, Bahareh
author_facet Asghari, Ali
Majidiani, Hamidreza
Nemati, Taher
Fatollahzadeh, Mohammad
Shams, Morteza
Naserifar, Razi
Kordi, Bahareh
author_sort Asghari, Ali
collection PubMed
description Toxoplasmosis is a global threat with significant zoonotic concern. The present in silico study was aimed at determination of bioinformatics features and immunogenic epitopes of a tyrosine-rich oocyst wall protein (TrOWP) of Toxoplasma gondii. After retrieving the amino acid sequence from UniProt database, several parameters were predicted including antigenicity, allergenicity, solubility and physico-chemical features, signal peptide, transmembrane domain, and posttranslational modifications. Following secondary and tertiary structure prediction, the 3D model was refined, and immunogenic epitopes were forecasted. It was a 25.57 kDa hydrophilic molecule with 236 residues, a signal peptide, and significant antigenicity scores. Moreover, several linear and conformational B-cell epitopes were present. Also, potential mouse and human cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes were predicted in the sequence. The findings of the present in silico study are promising as they render beneficial characteristics of TrOWP to be included in future vaccination experiments.
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spelling pubmed-85317822021-10-23 Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism Asghari, Ali Majidiani, Hamidreza Nemati, Taher Fatollahzadeh, Mohammad Shams, Morteza Naserifar, Razi Kordi, Bahareh Biomed Res Int Research Article Toxoplasmosis is a global threat with significant zoonotic concern. The present in silico study was aimed at determination of bioinformatics features and immunogenic epitopes of a tyrosine-rich oocyst wall protein (TrOWP) of Toxoplasma gondii. After retrieving the amino acid sequence from UniProt database, several parameters were predicted including antigenicity, allergenicity, solubility and physico-chemical features, signal peptide, transmembrane domain, and posttranslational modifications. Following secondary and tertiary structure prediction, the 3D model was refined, and immunogenic epitopes were forecasted. It was a 25.57 kDa hydrophilic molecule with 236 residues, a signal peptide, and significant antigenicity scores. Moreover, several linear and conformational B-cell epitopes were present. Also, potential mouse and human cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes were predicted in the sequence. The findings of the present in silico study are promising as they render beneficial characteristics of TrOWP to be included in future vaccination experiments. Hindawi 2021-10-14 /pmc/articles/PMC8531782/ /pubmed/34692826 http://dx.doi.org/10.1155/2021/1315618 Text en Copyright © 2021 Ali Asghari et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Asghari, Ali
Majidiani, Hamidreza
Nemati, Taher
Fatollahzadeh, Mohammad
Shams, Morteza
Naserifar, Razi
Kordi, Bahareh
Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism
title Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism
title_full Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism
title_fullStr Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism
title_full_unstemmed Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism
title_short Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism
title_sort toxoplasma gondii tyrosine-rich oocyst wall protein: a closer look through an in silico prism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531782/
https://www.ncbi.nlm.nih.gov/pubmed/34692826
http://dx.doi.org/10.1155/2021/1315618
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