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Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats

We analyzed urine samples from 191 cats for bacteriuria with an automated urine sediment analyzer (Idexx SediVue Dx), combined with image review by an observer, and compared to bacteriologic culture results. Sixty-nine samples were unambiguously assigned to be free of bacteria by the instrument and...

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Detalles Bibliográficos
Autores principales: Neubert, Elisabeth, Weber, Karin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531868/
https://www.ncbi.nlm.nih.gov/pubmed/34404287
http://dx.doi.org/10.1177/10406387211038918
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author Neubert, Elisabeth
Weber, Karin
author_facet Neubert, Elisabeth
Weber, Karin
author_sort Neubert, Elisabeth
collection PubMed
description We analyzed urine samples from 191 cats for bacteriuria with an automated urine sediment analyzer (Idexx SediVue Dx), combined with image review by an observer, and compared to bacteriologic culture results. Sixty-nine samples were unambiguously assigned to be free of bacteria by the instrument and the observer, and no bacterial growth was detected. Twenty-seven samples were unambiguously assigned to have bacteriuria; 24 of these 27 samples were culture-positive. For these samples, bacteriuria was predicted with a sensitivity of 100% and a specificity of 96%. A clear assignment was not possible for 95 samples, 81 of which were culture-negative. Specificity dropped to 45% when all samples were considered. Using the automated leukocyte count to predict bacteriuria, sensitivity was 82% and specificity was 75%. Automated sediment analysis is faster and less observer-dependent than sediment analysis under a microscope, but accurate detection of bacteriuria remains difficult in a large proportion of samples. Bacteriuria was significantly associated with leukocyte count; the leukocyte count was >5/high power field in 82% of culture-positive samples.
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spelling pubmed-85318682021-10-23 Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats Neubert, Elisabeth Weber, Karin J Vet Diagn Invest Brief Reports We analyzed urine samples from 191 cats for bacteriuria with an automated urine sediment analyzer (Idexx SediVue Dx), combined with image review by an observer, and compared to bacteriologic culture results. Sixty-nine samples were unambiguously assigned to be free of bacteria by the instrument and the observer, and no bacterial growth was detected. Twenty-seven samples were unambiguously assigned to have bacteriuria; 24 of these 27 samples were culture-positive. For these samples, bacteriuria was predicted with a sensitivity of 100% and a specificity of 96%. A clear assignment was not possible for 95 samples, 81 of which were culture-negative. Specificity dropped to 45% when all samples were considered. Using the automated leukocyte count to predict bacteriuria, sensitivity was 82% and specificity was 75%. Automated sediment analysis is faster and less observer-dependent than sediment analysis under a microscope, but accurate detection of bacteriuria remains difficult in a large proportion of samples. Bacteriuria was significantly associated with leukocyte count; the leukocyte count was >5/high power field in 82% of culture-positive samples. SAGE Publications 2021-08-17 2021-11 /pmc/articles/PMC8531868/ /pubmed/34404287 http://dx.doi.org/10.1177/10406387211038918 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Brief Reports
Neubert, Elisabeth
Weber, Karin
Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats
title Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats
title_full Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats
title_fullStr Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats
title_full_unstemmed Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats
title_short Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats
title_sort using the idexx sedivue dx to predict the need for urine bacteriologic culture in cats
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531868/
https://www.ncbi.nlm.nih.gov/pubmed/34404287
http://dx.doi.org/10.1177/10406387211038918
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