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Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway

As the number of patients with metabolic hypertension (MH) is increasing, there is an essential require for global measures to prevent and treat MH. Flavonoids such as buddleoside (BUD) from Chrysanthemum indicum L. are the main pharmacological components of cardiovascular activities. Previous studi...

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Autores principales: Wang, Ya-Jun, Su, Jie, Yu, Jing-Jing, Yan, Mei-Qiu, Shi, Meng-Lin, Huang, Qi-Di, Li, Bo, Wu, Wen-Yan, Xia, Rong-Shuang, Li, Si-Fan, Chen, Su-Hong, Lv, Gui-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532163/
https://www.ncbi.nlm.nih.gov/pubmed/34690786
http://dx.doi.org/10.3389/fphar.2021.755140
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author Wang, Ya-Jun
Su, Jie
Yu, Jing-Jing
Yan, Mei-Qiu
Shi, Meng-Lin
Huang, Qi-Di
Li, Bo
Wu, Wen-Yan
Xia, Rong-Shuang
Li, Si-Fan
Chen, Su-Hong
Lv, Gui-Yuan
author_facet Wang, Ya-Jun
Su, Jie
Yu, Jing-Jing
Yan, Mei-Qiu
Shi, Meng-Lin
Huang, Qi-Di
Li, Bo
Wu, Wen-Yan
Xia, Rong-Shuang
Li, Si-Fan
Chen, Su-Hong
Lv, Gui-Yuan
author_sort Wang, Ya-Jun
collection PubMed
description As the number of patients with metabolic hypertension (MH) is increasing, there is an essential require for global measures to prevent and treat MH. Flavonoids such as buddleoside (BUD) from Chrysanthemum indicum L. are the main pharmacological components of cardiovascular activities. Previous studies have suggested that the buddleoside-rich Chrysanthemum indicum L. extract (BUDE) can reduce blood pressure in spontaneously hypertensive rats (SHR). However, its effect on MH and how it works remains to be researched. In this study, it was observed that BUDE could lower blood pressure, improve dyslipidemia, and decrease the level of plasma LPS in MH rats. Moreover, BUDE improved intestinal flora and increased the expression of occludin and claudin-1 in the colon, and improved the pathological injury of the colon. Western bolt and qRT-PCR experiments showed that BUDE could down-regulate TLR4 and MyD88 protein and mRNA expression and inhibit phosphorylation of IKKβ, IκBα and NF-κB p65 in vessels of MH rats. These results showed that BUDE could regulate intestinal flora, improve intestinal barrier function, reduce the production and penetration of LPS, thereby inhibiting the vascular TLR4/MyD88 pathway, improving vascular endothelial function, and ultimately lowering blood pressure in MH rats. This study provides a new mechanism of BUDE against MH by inhibiting the enteric-origin LPS/TLR4 pathway.
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spelling pubmed-85321632021-10-23 Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway Wang, Ya-Jun Su, Jie Yu, Jing-Jing Yan, Mei-Qiu Shi, Meng-Lin Huang, Qi-Di Li, Bo Wu, Wen-Yan Xia, Rong-Shuang Li, Si-Fan Chen, Su-Hong Lv, Gui-Yuan Front Pharmacol Pharmacology As the number of patients with metabolic hypertension (MH) is increasing, there is an essential require for global measures to prevent and treat MH. Flavonoids such as buddleoside (BUD) from Chrysanthemum indicum L. are the main pharmacological components of cardiovascular activities. Previous studies have suggested that the buddleoside-rich Chrysanthemum indicum L. extract (BUDE) can reduce blood pressure in spontaneously hypertensive rats (SHR). However, its effect on MH and how it works remains to be researched. In this study, it was observed that BUDE could lower blood pressure, improve dyslipidemia, and decrease the level of plasma LPS in MH rats. Moreover, BUDE improved intestinal flora and increased the expression of occludin and claudin-1 in the colon, and improved the pathological injury of the colon. Western bolt and qRT-PCR experiments showed that BUDE could down-regulate TLR4 and MyD88 protein and mRNA expression and inhibit phosphorylation of IKKβ, IκBα and NF-κB p65 in vessels of MH rats. These results showed that BUDE could regulate intestinal flora, improve intestinal barrier function, reduce the production and penetration of LPS, thereby inhibiting the vascular TLR4/MyD88 pathway, improving vascular endothelial function, and ultimately lowering blood pressure in MH rats. This study provides a new mechanism of BUDE against MH by inhibiting the enteric-origin LPS/TLR4 pathway. Frontiers Media S.A. 2021-10-08 /pmc/articles/PMC8532163/ /pubmed/34690786 http://dx.doi.org/10.3389/fphar.2021.755140 Text en Copyright © 2021 Wang, Su, Yu, Yan, Shi, Huang, Li, Wu, Xia, Li, Chen and Lv. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Wang, Ya-Jun
Su, Jie
Yu, Jing-Jing
Yan, Mei-Qiu
Shi, Meng-Lin
Huang, Qi-Di
Li, Bo
Wu, Wen-Yan
Xia, Rong-Shuang
Li, Si-Fan
Chen, Su-Hong
Lv, Gui-Yuan
Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway
title Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway
title_full Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway
title_fullStr Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway
title_full_unstemmed Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway
title_short Buddleoside-Rich Chrysanthemum indicum L. Extract has a Beneficial Effect on Metabolic Hypertensive Rats by Inhibiting the Enteric-Origin LPS/TLR4 Pathway
title_sort buddleoside-rich chrysanthemum indicum l. extract has a beneficial effect on metabolic hypertensive rats by inhibiting the enteric-origin lps/tlr4 pathway
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532163/
https://www.ncbi.nlm.nih.gov/pubmed/34690786
http://dx.doi.org/10.3389/fphar.2021.755140
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