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Persistence of Escherichia coli in the microbiomes of red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) - does seed sanitization matter?

BACKGROUND: Seed sanitization via chemical processes removes/reduces microbes from the external surfaces of the seed and thereby could have an impact on the plants’ health or productivity. To determine the impact of seed sanitization on the plants’ microbiome and pathogen persistence, sanitized and...

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Autores principales: Dixit, Anirudha R., Khodadad, Christina L. M., Hummerick, Mary E., Spern, Cory J., Spencer, LaShelle E., Fischer, Jason A., Curry, Aaron B., Gooden, Jennifer L., Maldonado Vazquez, Gretchen J., Wheeler, Raymond M., Massa, Gioia D., Romeyn, Matthew W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532290/
https://www.ncbi.nlm.nih.gov/pubmed/34686151
http://dx.doi.org/10.1186/s12866-021-02345-5
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author Dixit, Anirudha R.
Khodadad, Christina L. M.
Hummerick, Mary E.
Spern, Cory J.
Spencer, LaShelle E.
Fischer, Jason A.
Curry, Aaron B.
Gooden, Jennifer L.
Maldonado Vazquez, Gretchen J.
Wheeler, Raymond M.
Massa, Gioia D.
Romeyn, Matthew W.
author_facet Dixit, Anirudha R.
Khodadad, Christina L. M.
Hummerick, Mary E.
Spern, Cory J.
Spencer, LaShelle E.
Fischer, Jason A.
Curry, Aaron B.
Gooden, Jennifer L.
Maldonado Vazquez, Gretchen J.
Wheeler, Raymond M.
Massa, Gioia D.
Romeyn, Matthew W.
author_sort Dixit, Anirudha R.
collection PubMed
description BACKGROUND: Seed sanitization via chemical processes removes/reduces microbes from the external surfaces of the seed and thereby could have an impact on the plants’ health or productivity. To determine the impact of seed sanitization on the plants’ microbiome and pathogen persistence, sanitized and unsanitized seeds from two leafy green crops, red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) were exposed to Escherichia coli and grown in controlled environment growth chambers simulating environmental conditions aboard the International Space Station. Plants were harvested at four intervals from 7 days post-germination to maturity. The bacterial communities of leaf and root were investigated using the 16S rRNA sequencing while quantitative polymerase chain reaction (qPCR) and heterotrophic plate counts were used to reveal the persistence of E. coli. RESULT: E. coli was detectable for longer periods of time in plants from sanitized versus unsanitized seeds and was identified in root tissue more frequently than in leaf tissue. 16S rRNA sequencing showed dynamic changes in the abundance of members of the phylum Proteobacteria, Firmicutes, and Bacteroidetes in leaf and root samples of both leafy crops. We observed minimal changes in the microbial diversity of lettuce or mizuna leaf tissue with time or between sanitized and unsanitized seeds. Beta-diversity showed that time had more of an influence on all samples versus the E. coli treatment. CONCLUSION: Our results indicated that the seed surface sanitization, a current requirement for sending seeds to space, could influence the microbiome. Insight into the changes in the crop microbiomes could lead to healthier plants and safer food supplementation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-021-02345-5.
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spelling pubmed-85322902021-10-25 Persistence of Escherichia coli in the microbiomes of red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) - does seed sanitization matter? Dixit, Anirudha R. Khodadad, Christina L. M. Hummerick, Mary E. Spern, Cory J. Spencer, LaShelle E. Fischer, Jason A. Curry, Aaron B. Gooden, Jennifer L. Maldonado Vazquez, Gretchen J. Wheeler, Raymond M. Massa, Gioia D. Romeyn, Matthew W. BMC Microbiol Research BACKGROUND: Seed sanitization via chemical processes removes/reduces microbes from the external surfaces of the seed and thereby could have an impact on the plants’ health or productivity. To determine the impact of seed sanitization on the plants’ microbiome and pathogen persistence, sanitized and unsanitized seeds from two leafy green crops, red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) were exposed to Escherichia coli and grown in controlled environment growth chambers simulating environmental conditions aboard the International Space Station. Plants were harvested at four intervals from 7 days post-germination to maturity. The bacterial communities of leaf and root were investigated using the 16S rRNA sequencing while quantitative polymerase chain reaction (qPCR) and heterotrophic plate counts were used to reveal the persistence of E. coli. RESULT: E. coli was detectable for longer periods of time in plants from sanitized versus unsanitized seeds and was identified in root tissue more frequently than in leaf tissue. 16S rRNA sequencing showed dynamic changes in the abundance of members of the phylum Proteobacteria, Firmicutes, and Bacteroidetes in leaf and root samples of both leafy crops. We observed minimal changes in the microbial diversity of lettuce or mizuna leaf tissue with time or between sanitized and unsanitized seeds. Beta-diversity showed that time had more of an influence on all samples versus the E. coli treatment. CONCLUSION: Our results indicated that the seed surface sanitization, a current requirement for sending seeds to space, could influence the microbiome. Insight into the changes in the crop microbiomes could lead to healthier plants and safer food supplementation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-021-02345-5. BioMed Central 2021-10-22 /pmc/articles/PMC8532290/ /pubmed/34686151 http://dx.doi.org/10.1186/s12866-021-02345-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Dixit, Anirudha R.
Khodadad, Christina L. M.
Hummerick, Mary E.
Spern, Cory J.
Spencer, LaShelle E.
Fischer, Jason A.
Curry, Aaron B.
Gooden, Jennifer L.
Maldonado Vazquez, Gretchen J.
Wheeler, Raymond M.
Massa, Gioia D.
Romeyn, Matthew W.
Persistence of Escherichia coli in the microbiomes of red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) - does seed sanitization matter?
title Persistence of Escherichia coli in the microbiomes of red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) - does seed sanitization matter?
title_full Persistence of Escherichia coli in the microbiomes of red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) - does seed sanitization matter?
title_fullStr Persistence of Escherichia coli in the microbiomes of red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) - does seed sanitization matter?
title_full_unstemmed Persistence of Escherichia coli in the microbiomes of red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) - does seed sanitization matter?
title_short Persistence of Escherichia coli in the microbiomes of red Romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and mizuna mustard (Brassica rapa var. japonica) - does seed sanitization matter?
title_sort persistence of escherichia coli in the microbiomes of red romaine lettuce (lactuca sativa cv. ‘outredgeous’) and mizuna mustard (brassica rapa var. japonica) - does seed sanitization matter?
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532290/
https://www.ncbi.nlm.nih.gov/pubmed/34686151
http://dx.doi.org/10.1186/s12866-021-02345-5
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