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Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds

The objectives of this work were to study the prevalence and the patterns of antibiotic resistance of staphylococcal isolates from bulk-tank milk of goat herds across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these herds and to evalu...

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Autores principales: Lianou, Daphne T., Petinaki, Efthymia, Cripps, Peter J., Gougoulis, Dimitris A., Michael, Charalambia K., Tsilipounidaki, Katerina, Skoulakis, Anargyros, Katsafadou, Angeliki I., Vasileiou, Natalia G. C., Giannoulis, Themis, Katsarou, Eleni I., Voidarou, Chrysoula, Mavrogianni, Vasia S., Caroprese, Mariangela, Fthenakis, George C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532619/
https://www.ncbi.nlm.nih.gov/pubmed/34680806
http://dx.doi.org/10.3390/antibiotics10101225
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author Lianou, Daphne T.
Petinaki, Efthymia
Cripps, Peter J.
Gougoulis, Dimitris A.
Michael, Charalambia K.
Tsilipounidaki, Katerina
Skoulakis, Anargyros
Katsafadou, Angeliki I.
Vasileiou, Natalia G. C.
Giannoulis, Themis
Katsarou, Eleni I.
Voidarou, Chrysoula
Mavrogianni, Vasia S.
Caroprese, Mariangela
Fthenakis, George C.
author_facet Lianou, Daphne T.
Petinaki, Efthymia
Cripps, Peter J.
Gougoulis, Dimitris A.
Michael, Charalambia K.
Tsilipounidaki, Katerina
Skoulakis, Anargyros
Katsafadou, Angeliki I.
Vasileiou, Natalia G. C.
Giannoulis, Themis
Katsarou, Eleni I.
Voidarou, Chrysoula
Mavrogianni, Vasia S.
Caroprese, Mariangela
Fthenakis, George C.
author_sort Lianou, Daphne T.
collection PubMed
description The objectives of this work were to study the prevalence and the patterns of antibiotic resistance of staphylococcal isolates from bulk-tank milk of goat herds across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these herds and to evaluate herd-related factors potentially associated with the presence of antibiotic resistance among these staphylococcal isolates. A cross-sectional study was performed on 119 goat herds in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant, resistant to at least one antibiotic, and multi-resistant staphylococcal isolates were recovered from 5.0%, 30.3%, and 16.0% of herds, respectively. Of 80 isolates, 7.5% were resistant to oxacillin, 50.0% were resistant to at least one antibiotic and 27.5% were multi-resistant. Resistance was seen more frequently among coagulase-negative staphylococci (59.3%) than among Staphylococcus aureus (23.8%). Resistance was more frequent against penicillin and ampicillin (41.3% of isolates) and fosfomycin (27.5%). No association was found with biofilm formation by staphylococci. For recovery of oxacillin-resistant isolates, the presence of working staff in the herds emerged as a significant factor; respective factors for the isolation of staphylococci resistant to at least one antibiotic were part-time farming and high (>10) number of systemic disinfections in the farm annually. The same three factors concurrently were also identified to be significant for the recovery of multi-resistant isolates.
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spelling pubmed-85326192021-10-23 Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds Lianou, Daphne T. Petinaki, Efthymia Cripps, Peter J. Gougoulis, Dimitris A. Michael, Charalambia K. Tsilipounidaki, Katerina Skoulakis, Anargyros Katsafadou, Angeliki I. Vasileiou, Natalia G. C. Giannoulis, Themis Katsarou, Eleni I. Voidarou, Chrysoula Mavrogianni, Vasia S. Caroprese, Mariangela Fthenakis, George C. Antibiotics (Basel) Article The objectives of this work were to study the prevalence and the patterns of antibiotic resistance of staphylococcal isolates from bulk-tank milk of goat herds across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these herds and to evaluate herd-related factors potentially associated with the presence of antibiotic resistance among these staphylococcal isolates. A cross-sectional study was performed on 119 goat herds in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant, resistant to at least one antibiotic, and multi-resistant staphylococcal isolates were recovered from 5.0%, 30.3%, and 16.0% of herds, respectively. Of 80 isolates, 7.5% were resistant to oxacillin, 50.0% were resistant to at least one antibiotic and 27.5% were multi-resistant. Resistance was seen more frequently among coagulase-negative staphylococci (59.3%) than among Staphylococcus aureus (23.8%). Resistance was more frequent against penicillin and ampicillin (41.3% of isolates) and fosfomycin (27.5%). No association was found with biofilm formation by staphylococci. For recovery of oxacillin-resistant isolates, the presence of working staff in the herds emerged as a significant factor; respective factors for the isolation of staphylococci resistant to at least one antibiotic were part-time farming and high (>10) number of systemic disinfections in the farm annually. The same three factors concurrently were also identified to be significant for the recovery of multi-resistant isolates. MDPI 2021-10-08 /pmc/articles/PMC8532619/ /pubmed/34680806 http://dx.doi.org/10.3390/antibiotics10101225 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lianou, Daphne T.
Petinaki, Efthymia
Cripps, Peter J.
Gougoulis, Dimitris A.
Michael, Charalambia K.
Tsilipounidaki, Katerina
Skoulakis, Anargyros
Katsafadou, Angeliki I.
Vasileiou, Natalia G. C.
Giannoulis, Themis
Katsarou, Eleni I.
Voidarou, Chrysoula
Mavrogianni, Vasia S.
Caroprese, Mariangela
Fthenakis, George C.
Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds
title Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds
title_full Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds
title_fullStr Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds
title_full_unstemmed Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds
title_short Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds
title_sort prevalence, patterns, association with biofilm formation, effects on milk quality and risk factors for antibiotic resistance of staphylococci from bulk-tank milk of goat herds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532619/
https://www.ncbi.nlm.nih.gov/pubmed/34680806
http://dx.doi.org/10.3390/antibiotics10101225
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