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Imipenem Resistance Mediated by bla(OXA-913) Gene in Pseudomonas aeruginosa
Treatment of infectious diseases caused by carbapenem-resistant Pseudomonas aeruginosa is becoming a greater challenge. This study aimed to identify the imipenem resistance mechanism in P. aeruginosa isolated from a dog. Minimum Inhibitory Concentration (MIC) was determined by the broth microdilutio...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532623/ https://www.ncbi.nlm.nih.gov/pubmed/34680769 http://dx.doi.org/10.3390/antibiotics10101188 |
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author | Moon, Dong-Chan Mechesso, Abraham Fikru Kang, Hee-Young Kim, Su-Jeong Choi, Ji-Hyun Song, Hyun-Ju Yoon, Soon-Seek Lim, Suk-Kyung |
author_facet | Moon, Dong-Chan Mechesso, Abraham Fikru Kang, Hee-Young Kim, Su-Jeong Choi, Ji-Hyun Song, Hyun-Ju Yoon, Soon-Seek Lim, Suk-Kyung |
author_sort | Moon, Dong-Chan |
collection | PubMed |
description | Treatment of infectious diseases caused by carbapenem-resistant Pseudomonas aeruginosa is becoming a greater challenge. This study aimed to identify the imipenem resistance mechanism in P. aeruginosa isolated from a dog. Minimum Inhibitory Concentration (MIC) was determined by the broth microdilution method according to the Clinical and Laboratory Standards Institute recommendations. We performed polymerase chain reaction and whole-genome sequencing to detect carbapenem resistance genes. Genomic DNA of P. aeruginosa K19PSE24 was sequenced via the combined analysis of 20-kb PacBio SMRTbell and PacBio RS II. Peptide-Peptide Nucleic Acid conjugates (P-PNAs) targeting the translation initiation region of bla(OXA-913) were synthesized. The isolate (K19PSE24) was resistant to imipenem and piperacillin/tazobactam yet was susceptible to most of the tested antimicrobials. Whole-genome sequencing revealed that the K19PSE24 genome comprised a single contig amounting to 6,815,777 base pairs, with 65 tRNA and 12 rRNA genes. K19PSE24 belonged to sequence type 313 and carried the genes aph(3)-IIb, fosA, catB7, crpP, and bla(OXA-913) (an allele deposited in GenBank but not described in the literature). K19PSE24 also carried genes encoding for virulence factors (exoenzyme T, exotoxin A, and elastase B) that are associated with adhesion, invasion, and tissue lysis. Nevertheless, we did not detect any of the previously reported carbapenem resistance genes. This is the first report of the bla(OXA-913) gene in imipenem-resistant P. aeruginosa in the literature. Notably, no viable colonies were found after co-treatment with imipenem (2 µg/mL) and either of the P-PNAs (12.5 µM or 25 µM). The imipenem resistance in K19PSE24 was primarily due to bla(OXA-913) gene carriage. |
format | Online Article Text |
id | pubmed-8532623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85326232021-10-23 Imipenem Resistance Mediated by bla(OXA-913) Gene in Pseudomonas aeruginosa Moon, Dong-Chan Mechesso, Abraham Fikru Kang, Hee-Young Kim, Su-Jeong Choi, Ji-Hyun Song, Hyun-Ju Yoon, Soon-Seek Lim, Suk-Kyung Antibiotics (Basel) Communication Treatment of infectious diseases caused by carbapenem-resistant Pseudomonas aeruginosa is becoming a greater challenge. This study aimed to identify the imipenem resistance mechanism in P. aeruginosa isolated from a dog. Minimum Inhibitory Concentration (MIC) was determined by the broth microdilution method according to the Clinical and Laboratory Standards Institute recommendations. We performed polymerase chain reaction and whole-genome sequencing to detect carbapenem resistance genes. Genomic DNA of P. aeruginosa K19PSE24 was sequenced via the combined analysis of 20-kb PacBio SMRTbell and PacBio RS II. Peptide-Peptide Nucleic Acid conjugates (P-PNAs) targeting the translation initiation region of bla(OXA-913) were synthesized. The isolate (K19PSE24) was resistant to imipenem and piperacillin/tazobactam yet was susceptible to most of the tested antimicrobials. Whole-genome sequencing revealed that the K19PSE24 genome comprised a single contig amounting to 6,815,777 base pairs, with 65 tRNA and 12 rRNA genes. K19PSE24 belonged to sequence type 313 and carried the genes aph(3)-IIb, fosA, catB7, crpP, and bla(OXA-913) (an allele deposited in GenBank but not described in the literature). K19PSE24 also carried genes encoding for virulence factors (exoenzyme T, exotoxin A, and elastase B) that are associated with adhesion, invasion, and tissue lysis. Nevertheless, we did not detect any of the previously reported carbapenem resistance genes. This is the first report of the bla(OXA-913) gene in imipenem-resistant P. aeruginosa in the literature. Notably, no viable colonies were found after co-treatment with imipenem (2 µg/mL) and either of the P-PNAs (12.5 µM or 25 µM). The imipenem resistance in K19PSE24 was primarily due to bla(OXA-913) gene carriage. MDPI 2021-09-29 /pmc/articles/PMC8532623/ /pubmed/34680769 http://dx.doi.org/10.3390/antibiotics10101188 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Moon, Dong-Chan Mechesso, Abraham Fikru Kang, Hee-Young Kim, Su-Jeong Choi, Ji-Hyun Song, Hyun-Ju Yoon, Soon-Seek Lim, Suk-Kyung Imipenem Resistance Mediated by bla(OXA-913) Gene in Pseudomonas aeruginosa |
title | Imipenem Resistance Mediated by bla(OXA-913) Gene in Pseudomonas aeruginosa |
title_full | Imipenem Resistance Mediated by bla(OXA-913) Gene in Pseudomonas aeruginosa |
title_fullStr | Imipenem Resistance Mediated by bla(OXA-913) Gene in Pseudomonas aeruginosa |
title_full_unstemmed | Imipenem Resistance Mediated by bla(OXA-913) Gene in Pseudomonas aeruginosa |
title_short | Imipenem Resistance Mediated by bla(OXA-913) Gene in Pseudomonas aeruginosa |
title_sort | imipenem resistance mediated by bla(oxa-913) gene in pseudomonas aeruginosa |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532623/ https://www.ncbi.nlm.nih.gov/pubmed/34680769 http://dx.doi.org/10.3390/antibiotics10101188 |
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