A Survey of Intestinal Helminths of Dogs in Slovakia with an Emphasis on Zoonotic Species

SIMPLE SUMMARY: Dogs are the most popular pet animals worldwide; however, frequent and close contact with people increases the risk of transmission of different zoonotic parasites. As the occurrence of intestinal parasites in the dog population is affected by several factors, understanding the epidemiology of zoonotic parasitic infections is important to minimize the risks for humans. This study presents results about the prevalence of gastrointestinal helminths in seven different groups of dogs (pet, shelter, guard, working, and hunting dogs, as well as dogs from segregated Roma settlements) in Slovakia. Out of 495 faecal samples collected between 2016 and 2021, eggs of intestinal helminths were detected in 134 (27.1%) samples. Altogether, six different species/genera/families, namely, Toxocara canis (14.7%), Toxascaris leonina (1.6%), Trichuris vulpis (6.3%), Capillaria spp. (1.4%), Ancylostoma/Uncinaria spp. (8.3%), and taeniid eggs (4.0%), were recorded. Infection with Echinococcus multilocularis was confirmed in 2.2% of dogs and 0.4% of the animals were infested with Taenia hydatigena. The results showed that the occurrence of intestinal helminths is quite frequent in the majority of analyzed dog groups, with a close correlation between the occurrence of intestinal helminths and availability of veterinary care and anthelmintic therapy. ABSTRACT: Dogs are the most popular pets worldwide; however, close contact with people increases the risk of transmission of different zoonotic parasites. This study aims to determine the prevalence of gastrointestinal helminths in dogs in Slovakia. A total of 495 faecal samples collected from pet, shelter, guard, working (police), and hunting dogs, as well as dogs from segregated Roma settlements between 2016 and 2021, were examined using flotation and molecular methods. Eggs of intestinal helminths were detected in 134 (27.1%) samples. Microscopically, six different species/genera/families, namely, Toxocara canis (14.7%), Toxascaris leonina (1.6%), Trichuris vulpis (6.3%), Capillaria spp. (1.4%), Ancylostoma/Uncinaria spp. (8.3%), and taeniid eggs (4.0%), were recorded. Molecular analyses revealed infection with Echinococcus multilocularis in 2.2% of dogs and 0.4% of the animals were infected with Taenia hydatigena. The results showed a correlation between the occurrence of intestinal helminths and the availability of veterinary care, as dogs from Roma settlements and shelter dogs were the most often infected (66.7% and 39.2%, respectively). On the other hand, working animals were in the best health condition, with only 2.5% being positive. The relatively frequent occurrence of zoonotic species points to the constant need for preventive measures and regular deworming of dogs.