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The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37

(1) Background: The cathelicidin peptide LL-37 is a prominent molecule with many biological activities, including antimicrobial. Due to its importance, here, we describe the production of LL-37 tagged with SmbP, a relatively new carrier protein that improves the production of recombinant proteins an...

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Autores principales: Perez-Perez, David A., Villanueva-Ramirez, Teresa de J., Hernandez-Pedraza, Adriana E., Casillas-Vega, Nestor G., Gonzalez-Barranco, Patricia, Zarate, Xristo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532731/
https://www.ncbi.nlm.nih.gov/pubmed/34680851
http://dx.doi.org/10.3390/antibiotics10101271
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author Perez-Perez, David A.
Villanueva-Ramirez, Teresa de J.
Hernandez-Pedraza, Adriana E.
Casillas-Vega, Nestor G.
Gonzalez-Barranco, Patricia
Zarate, Xristo
author_facet Perez-Perez, David A.
Villanueva-Ramirez, Teresa de J.
Hernandez-Pedraza, Adriana E.
Casillas-Vega, Nestor G.
Gonzalez-Barranco, Patricia
Zarate, Xristo
author_sort Perez-Perez, David A.
collection PubMed
description (1) Background: The cathelicidin peptide LL-37 is a prominent molecule with many biological activities, including antimicrobial. Due to its importance, here, we describe the production of LL-37 tagged with SmbP, a relatively new carrier protein that improves the production of recombinant proteins and peptides in Escherichia coli. We present an alternative method for the rapid expression, purification, and antimicrobial evaluation of LL-37, that involves only one purification step. (2) Methods: A DNA construct of SmbP_LL-37 was transformed into E. coli BL21(DE3); after overnight expression, the protein was purified directly from the cell lysate using immobilized metal-affinity chromatography. SmbP_LL-37 was treated with Enterokinase to obtain the free LL-37 peptide. The antimicrobial activity of both SmbP_LL-37 and free LL-37 was determined using the colony forming unit assay method. (3) Results: SmbP_LL-37 was observed in the soluble fraction of the cell lysate; after purification with IMAC, protein gel electrophoresis, and analysis by ImageJ, it showed 90% purity. A total of 3.6 mg of SmbP_LL-37 was produced from one liter of cell culture. SmbP_LL-37 and free LL-37 both showed inhibition activity against Staphylococcus aureus and Escherichia coli. (4) Conclusions: The SmbP fusion protein is a valuable tool for producing biologically-active LL-37 peptide. The production method described here should be of interest for the expression and purification of additional cationic peptides, since it cuts the purification time considerably prior to determination of antimicrobial activity.
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spelling pubmed-85327312021-10-23 The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37 Perez-Perez, David A. Villanueva-Ramirez, Teresa de J. Hernandez-Pedraza, Adriana E. Casillas-Vega, Nestor G. Gonzalez-Barranco, Patricia Zarate, Xristo Antibiotics (Basel) Article (1) Background: The cathelicidin peptide LL-37 is a prominent molecule with many biological activities, including antimicrobial. Due to its importance, here, we describe the production of LL-37 tagged with SmbP, a relatively new carrier protein that improves the production of recombinant proteins and peptides in Escherichia coli. We present an alternative method for the rapid expression, purification, and antimicrobial evaluation of LL-37, that involves only one purification step. (2) Methods: A DNA construct of SmbP_LL-37 was transformed into E. coli BL21(DE3); after overnight expression, the protein was purified directly from the cell lysate using immobilized metal-affinity chromatography. SmbP_LL-37 was treated with Enterokinase to obtain the free LL-37 peptide. The antimicrobial activity of both SmbP_LL-37 and free LL-37 was determined using the colony forming unit assay method. (3) Results: SmbP_LL-37 was observed in the soluble fraction of the cell lysate; after purification with IMAC, protein gel electrophoresis, and analysis by ImageJ, it showed 90% purity. A total of 3.6 mg of SmbP_LL-37 was produced from one liter of cell culture. SmbP_LL-37 and free LL-37 both showed inhibition activity against Staphylococcus aureus and Escherichia coli. (4) Conclusions: The SmbP fusion protein is a valuable tool for producing biologically-active LL-37 peptide. The production method described here should be of interest for the expression and purification of additional cationic peptides, since it cuts the purification time considerably prior to determination of antimicrobial activity. MDPI 2021-10-19 /pmc/articles/PMC8532731/ /pubmed/34680851 http://dx.doi.org/10.3390/antibiotics10101271 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Perez-Perez, David A.
Villanueva-Ramirez, Teresa de J.
Hernandez-Pedraza, Adriana E.
Casillas-Vega, Nestor G.
Gonzalez-Barranco, Patricia
Zarate, Xristo
The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37
title The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37
title_full The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37
title_fullStr The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37
title_full_unstemmed The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37
title_short The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37
title_sort small metal-binding protein smbp simplifies the recombinant expression and purification of the antimicrobial peptide ll-37
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532731/
https://www.ncbi.nlm.nih.gov/pubmed/34680851
http://dx.doi.org/10.3390/antibiotics10101271
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