Distribution of Deer Keds (Diptera: Hippoboscidae) in Free-Living Cervids of the Tuscan-Emilian Apennines, Central Italy, and Establishment of the Allochthonous Ectoparasite Lipoptena fortisetosa

SIMPLE SUMMARY: In recent years, the increased presence of wildlife in habitats close to urban settlements has raised concerns about the risk of pathogen transmission from wild animals to humans due to the spread of different parasites. For this reason, a survey aimed at describing the dispersal and parasitism level of two cervid ectoparasites was carried out in the northern Apennines, in central Italy. The presence of two hippoboscids, the autochthonous Lipoptena cervi and allochthonous L. fortisetosa, native to Eastern Asia and recently recorded in Italy, were assessed on their main host species (red deer, fallow deer, and roe deer), considering host sex and age. The alien species L. fortisetosa was found to be widespread in the study area, most likely competing with L. cervi. Moreover, red deer seemed to be the favored host of both flies, with differences in sex and age class preferences. This study demonstrated the importance of regularly monitoring the populations of these parasites, especially the invasive species, due to the risks to human health, as these insects are potential vectors of pathogens. ABSTRACT: Lipoptena fortisetosa and L. cervi are hematophagous ectoparasites belonging to the Hippoboscidae family and preferentially living on cervids. In recent years, they have received specific attention due to the great increase in the abundance of their host species, and to their medical and veterinary importance as possible vectors of pathogens harmful to humans and animals. The aim of this study was to investigate the parasitism level of both of these flies on their main hosts in Italy, which are red deer, fallow deer, and roe deer, and to highlight a possible preference for a species, sex, or age class among the hosts. Deer keds were collected by examining 326 cervids hunted in the Tuscan-Emilian Apennines. Outcomes showed that L. fortisetosa has greatly spread throughout the study area, where it competes with the autochthonous L. cervi. Moreover, red deer was the favored host species of both ectoparasites, while different preferences for host sex and age classes were observed in the two hippoboscids. The regular monitoring of deer ked populations, especially the allochthonous L. fortisetosa, which is continuously spreading in Europe, is recommended to expand the knowledge on these parasitic species that are potentially dangerous to public health.