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Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland)

Resistance of bacteria, fungi and cancer cells to antibiotics and other drugs is recognized as one of the major problems in current medicine. Therefore, a search for new biologically active compounds able to either kill pathogenic cells or inhibit their growth is mandatory. Hard-to-reach habitats ap...

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Autores principales: Jaroszewicz, Weronika, Bielańska, Patrycja, Lubomska, Daria, Kosznik-Kwaśnicka, Katarzyna, Golec, Piotr, Grabowski, Łukasz, Wieczerzak, Ewa, Dróżdż, Weronika, Gaffke, Lidia, Pierzynowska, Karolina, Węgrzyn, Grzegorz, Węgrzyn, Alicja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532742/
https://www.ncbi.nlm.nih.gov/pubmed/34680793
http://dx.doi.org/10.3390/antibiotics10101212
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author Jaroszewicz, Weronika
Bielańska, Patrycja
Lubomska, Daria
Kosznik-Kwaśnicka, Katarzyna
Golec, Piotr
Grabowski, Łukasz
Wieczerzak, Ewa
Dróżdż, Weronika
Gaffke, Lidia
Pierzynowska, Karolina
Węgrzyn, Grzegorz
Węgrzyn, Alicja
author_facet Jaroszewicz, Weronika
Bielańska, Patrycja
Lubomska, Daria
Kosznik-Kwaśnicka, Katarzyna
Golec, Piotr
Grabowski, Łukasz
Wieczerzak, Ewa
Dróżdż, Weronika
Gaffke, Lidia
Pierzynowska, Karolina
Węgrzyn, Grzegorz
Węgrzyn, Alicja
author_sort Jaroszewicz, Weronika
collection PubMed
description Resistance of bacteria, fungi and cancer cells to antibiotics and other drugs is recognized as one of the major problems in current medicine. Therefore, a search for new biologically active compounds able to either kill pathogenic cells or inhibit their growth is mandatory. Hard-to-reach habitats appear to be unexplored sources of microorganisms producing previously unknown antibiotics and other molecules revealing potentially therapeutic properties. Caves belong to such habitats, and Actinobacteria are a predominant group of microorganisms occurring there. This group of bacteria are known for production of many antibiotics and other bioactive compounds. Interestingly, it was demonstrated previously that infection with bacteriophages might enhance production of antibiotics by them. Here, we describe a series of newly isolated strains of Actinobacteria that were found in caves from the Tatra Mountains (Poland). Phage induction tests indicated that some of them may bear active prophages able to produce virions upon treatment with mitomycin C or UV irradiation. Among all the examined bacteria, two newly isolated Streptomyces sp. strains were further characterized to demonstrate their ability to inhibit the growth of pathogenic bacteria (strains of Staphylococcus aureus, Salmonella enterica, Enterococcus sp., Escherichia coli, and Pseudomonas aeruginosa) and fungi (different species and strains from the genus Candida). Moreover, extracts from these Streptomyces strains reduced viability of the breast-cancer cell line T47D. Chemical analyses of these extracts indicated the presence of isomers of dichloranthrabenzoxocinone and 4,10- or 10,12-dichloro-3-O-methylanthrabenzoxocinone, which are putative antimicrobial compounds. Moreover, various previously unknown (unclassified) molecules were also detected using liquid chromatography–mass spectrometry, suggesting that tested Streptomyces strains may synthesize a battery of bioactive compounds with antibacterial, antifungal, and anticancer activities. These results indicate that further studies on the newly isolated Actinobacteria might be a promising approach to develop novel antibacterial, antifungal, and/or anticancer drugs.
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spelling pubmed-85327422021-10-23 Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland) Jaroszewicz, Weronika Bielańska, Patrycja Lubomska, Daria Kosznik-Kwaśnicka, Katarzyna Golec, Piotr Grabowski, Łukasz Wieczerzak, Ewa Dróżdż, Weronika Gaffke, Lidia Pierzynowska, Karolina Węgrzyn, Grzegorz Węgrzyn, Alicja Antibiotics (Basel) Article Resistance of bacteria, fungi and cancer cells to antibiotics and other drugs is recognized as one of the major problems in current medicine. Therefore, a search for new biologically active compounds able to either kill pathogenic cells or inhibit their growth is mandatory. Hard-to-reach habitats appear to be unexplored sources of microorganisms producing previously unknown antibiotics and other molecules revealing potentially therapeutic properties. Caves belong to such habitats, and Actinobacteria are a predominant group of microorganisms occurring there. This group of bacteria are known for production of many antibiotics and other bioactive compounds. Interestingly, it was demonstrated previously that infection with bacteriophages might enhance production of antibiotics by them. Here, we describe a series of newly isolated strains of Actinobacteria that were found in caves from the Tatra Mountains (Poland). Phage induction tests indicated that some of them may bear active prophages able to produce virions upon treatment with mitomycin C or UV irradiation. Among all the examined bacteria, two newly isolated Streptomyces sp. strains were further characterized to demonstrate their ability to inhibit the growth of pathogenic bacteria (strains of Staphylococcus aureus, Salmonella enterica, Enterococcus sp., Escherichia coli, and Pseudomonas aeruginosa) and fungi (different species and strains from the genus Candida). Moreover, extracts from these Streptomyces strains reduced viability of the breast-cancer cell line T47D. Chemical analyses of these extracts indicated the presence of isomers of dichloranthrabenzoxocinone and 4,10- or 10,12-dichloro-3-O-methylanthrabenzoxocinone, which are putative antimicrobial compounds. Moreover, various previously unknown (unclassified) molecules were also detected using liquid chromatography–mass spectrometry, suggesting that tested Streptomyces strains may synthesize a battery of bioactive compounds with antibacterial, antifungal, and anticancer activities. These results indicate that further studies on the newly isolated Actinobacteria might be a promising approach to develop novel antibacterial, antifungal, and/or anticancer drugs. MDPI 2021-10-05 /pmc/articles/PMC8532742/ /pubmed/34680793 http://dx.doi.org/10.3390/antibiotics10101212 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jaroszewicz, Weronika
Bielańska, Patrycja
Lubomska, Daria
Kosznik-Kwaśnicka, Katarzyna
Golec, Piotr
Grabowski, Łukasz
Wieczerzak, Ewa
Dróżdż, Weronika
Gaffke, Lidia
Pierzynowska, Karolina
Węgrzyn, Grzegorz
Węgrzyn, Alicja
Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland)
title Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland)
title_full Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland)
title_fullStr Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland)
title_full_unstemmed Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland)
title_short Antibacterial, Antifungal and Anticancer Activities of Compounds Produced by Newly Isolated Streptomyces Strains from the Szczelina Chochołowska Cave (Tatra Mountains, Poland)
title_sort antibacterial, antifungal and anticancer activities of compounds produced by newly isolated streptomyces strains from the szczelina chochołowska cave (tatra mountains, poland)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532742/
https://www.ncbi.nlm.nih.gov/pubmed/34680793
http://dx.doi.org/10.3390/antibiotics10101212
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