Characterization, Density and In Vitro Controlled Release Properties of Mimosa (Acacia mearnsii) Tannin Encapsulated in Palm and Sunflower Oils

SIMPLE SUMMARY: The utilization of tannin in mitigating enteric methane suffers a setback in terms of dietary intake and digestibility because of the tannin’s astringency and instability in the gastrointestinal tract. Microencapsulation of tannin using lipids could mask its bitter taste and ensure its controlled release at the target site. This study aimed to encapsulate Acacia mearnsii tannin extract with palm and sunflower oils, and to evaluate the efficacy of the encapsulated tannins with regards to encapsulation efficiency, density, and release of tannin in media, simulating the rumen, abomasum and the small intestine. Mimosa tannin was encapsulated in palm oil or sunflower oil using a double emulsion method. The findings showed that encapsulated mimosa tannins in the palm oil and sunflower oil had high encapsulation efficiencies with smaller sizes and were lower in density compared to the unencapsulated mimosa tannin. The amount of tannins released by the unencapsulated tannin after 24 h in rumen (94%), abomasum (92%) and small intestine (96%) simulated buffers, were reduced to 24%, 21% and 19%, respectively, for the sunflower oil microparticle and 18%, 20% and 16%, respectively, for the palm oil microparticle in the same buffers and periods. Palm oil and sunflower oil successfully encapsulated the mimosa tannin and controlled its release in the gastrointestinal tract simulated media without compromising rumen fermentation. ABSTRACT: Tannin has gained wider acceptance as a dietary supplement in contemporary animal nutrition investigations because of its potential to reduce enteric methane emission. However, a major drawback to dietary tannin intake is the bitter taste and instability in the gastrointestinal tract (GIT). The utilization of fats as coating materials will ensure appropriate masking of the tannin’s aversive taste and its delivery to the target site. The aims of this study were to encapsulate mimosa tannin with palm oil or sunflower oil, and to assess the microcapsules in terms of encapsulation efficiency, morphology, density, and in vitro release of tannin in media simulating the rumen (pH 5.6), abomasum (pH 2.9) and small intestine (pH 7.4). The microencapsulation of mimosa tannin in palm or sunflower oils was accomplished using a double emulsion technique. The results revealed that encapsulated mimosa tannins in palm oil (EMT(P)) and sunflower oil (EMT(S)) had high yields (59% vs. 58%) and encapsulation efficiencies (70% vs. 68%), respectively. Compared to unencapsulated mimosa tannin (UMT), the morphology showed that the encapsulated tannins were smaller in size and spherical in shape. The UMT had (p < 0.01) higher particle density (1.44 g/cm(3)) compared to 1.22 g/cm(3) and 1.21 g/cm(3) for the EMT(S) and EMT(P), respectively. The proportion of tannins released by the UMT after 24 h in the rumen (94%), abomasum (92%) and small intestine (96%) simulated buffers, reduced (p < 0.01) to 24%, 21% and 19% for the EMT(S) and 18%, 20% and 16% for the EMT(P) in similar media and timeframe. The release kinetics for the encapsulated tannins was slow and steady, thus, best fitted by the Higuchi model while the UMT dissolved quickly, hence, only fitted to a First order model. Sequential tannin release also indicated that the EMT(S) and EMT(P) were stable across the GIT. It was concluded that the microencapsulation of mimosa tannin in palm or sunflower oils stabilized tannins release in the GIT simulated buffers with the potential to modify rumen fermentation. Further studies should be conducted on the palm and sunflower oils microcapsules’ lipid stability, fatty acid transfer rate in the GIT and antioxidant properties of the encapsulated tannins.