Genetic Variations and Haplotypic Diversity in the Myostatin Gene of Different Cattle Breeds in Russia

SIMPLE SUMMARY: This paper presents the results of the study of two polymorphisms of the myostatin gene associated with muscular hypertrophy in the Russian populations of Aberdeen Angus, Limousin, Simmental, and Belgian Blue cattle breeds. For their diagnostics, test systems based on modern molecular genetic methods were developed, and the population analysis showed a low frequency of the undesirable allele associated with the genetic defect of double-muscling, and a high frequency of the allele that presumably positively influences meat productivity traits. ABSTRACT: The myostatin gene (MSTN) in cattle has a number of polymorphisms associated with increased muscle mass. The aim of the current study was to determine the haplotype frequencies of F94L and nt821(del11) MSTN polymorphisms among cattle bred for meat in Russia, using DNA analysis. Using the earlier created test systems based on the AS-PCR and PCR-RFLP methods, six populations of Aberdeen Angus (n = 684), two populations of Limousin (n = 54), one population of Simmental (n = 55), and one population of Belgian Blue (n = 137) belonging to Russian farms were genotyped on nt821(del11) and F94L MSTN polymorphisms. The animal carriers of the mutant allele of nt821(del11) MSTN associated with the double-muscling genetic defect were found in one Aberdeen Angus population at a frequency of 2.18%, but were not found in the Limousin and Simmental populations. However, 100% of the Belgian Blue population were heterozygous carriers of nt821(del11) MSTN. The frequencies of the A allele F94L MSTN desirable for productivity traits in the Limousin populations were the highest and accounted for 0.97 and 1 in populations one and two, while in the Aberdeen Angus, Simmental, and Belgian Blue populations, these figures were considerably lower at 0.04–0.08, depending on the population. The obtained data show the high genetic potential of Russian beef cattle, and facilitate an improvement in meat productivity by preserving the health of animals.