Comparison of the Automatic and Manual Broiler Pre-Slaughter Chain Based on Trailer Microclimate during Transportation and Its Effect on m. pectoralis major

SIMPLE SUMMARY: Improper pre-slaughter catching, loading/unloading, handling, and transport may cause negative effects on the welfare and meat quality of poultry. During the catch process, noisy, rough, or aggressive techniques can cause birds to panic, which can lead to injuries and lower meat quality. Fractures, joint dislocations, and bruises can be common and cause bird suffering, mortality, carcass degradation, and economic loss. Proper pre-slaughter processes can ensure the safety of poultry and workers. One way to prevent these problems is to use automatic catching machines (harvesters/chicken cat), handling machines (shuttles), and air-conditioned trucks (trailers) to collect and handle poultry. Microclimate in trailers is another important factor influencing welfare. Internal overheating may cause high mortality of the animals during transport and reduced meat quality. The use of modern air-conditioned trailers results in improved welfare conditions, reduced mortality and the incidence of injuries and fractures, and increased meat quality. ABSTRACT: This study aims to compare two broiler pre-slaughter chain methods: (i) the automatic pre-slaughter chain (APC) and (ii) manual pre-slaughter chain (MPC). The comparison is based on the evaluation of the trailer microclimate, number of injuries, and breast muscle (m. pectoralis major) quality. Transportation lasts 3.5 h, unloading 1 h. The selection of two hundred 39-day-old broilers (Ross 308 and Cobb 500 breeds) is random for each type of method. After slaughter, the pH value, electrical conductivity (EC), and color (lightness) of breast muscle tissues are determined at different post-mortem intervals. The MPC negatively affects the microclimate (p < 0.001), meat qualitative characteristics (p < 0.001), and places a greater strain on the body of chickens compared with APC. The average pH(15min) value of MPC broiler breast muscle tissue, generally used as the main meat quality parameter, is 5.97 ± 0.12, in contrast to 6.36 ± 0.16 for APC. Higher pH(15min) value of APC indicates better welfare and pre-slaughter handling. Values of EC and L* of breast tissues also confirms a difference between the methods of broiler handling (p < 0.001). No difference is found between the breed lines (p > 0.05).