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Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas
SIMPLE SUMMARY: Mouse mammary tumour virus-like (MMTV-like) is suspected to be involved in human breast cancer and feline mammary carcinomas (FMCs). We previously reported the identification of MMTV-like sequences and viral protein in six of 78 FMCs collected in Tuscany, Italy. To corroborate this f...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532932/ https://www.ncbi.nlm.nih.gov/pubmed/34679842 http://dx.doi.org/10.3390/ani11102821 |
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author | Parisi, Francesca Muscatello, Luisa Vera Civita, Prospero Lessi, Francesca Menicagli, Michele Millanta, Francesca Brunetti, Barbara Benazzi, Cinzia Sarli, Giuseppe Freer, Giulia Pistello, Mauro Mazzanti, Chiara Maria Poli, Alessandro |
author_facet | Parisi, Francesca Muscatello, Luisa Vera Civita, Prospero Lessi, Francesca Menicagli, Michele Millanta, Francesca Brunetti, Barbara Benazzi, Cinzia Sarli, Giuseppe Freer, Giulia Pistello, Mauro Mazzanti, Chiara Maria Poli, Alessandro |
author_sort | Parisi, Francesca |
collection | PubMed |
description | SIMPLE SUMMARY: Mouse mammary tumour virus-like (MMTV-like) is suspected to be involved in human breast cancer and feline mammary carcinomas (FMCs). We previously reported the identification of MMTV-like sequences and viral protein in six of 78 FMCs collected in Tuscany, Italy. To corroborate this finding, FMCs samples collected from a different geographic area were investigated. MMTV-like sequences and p14 protein were identified in three of 24 FMCs collected at the University of Bologna, one tubular carcinoma, one tubulopapillary carcinoma and one ductal carcinoma. All the examined FMCs from Pisa and Bologna were submitted to immunohistochemistry for molecular phenotype characterization. Of the nine positive FMCs, six were basal-like and three luminal-like. This study highlights the presence of MMTV-like sequences and protein in FMCs of different geographic areas. The characterization of molecular phenotype could contribute to understand the possible role of MMTV-like virus in FMC biological behaviour. ABSTRACT: In the last few years MMTV-like nucleotide sequences were detected in some feline and canine mammary tumours. Due to the confirmed role of cats in the epidemiology of the MMTV-like virus, the aim of this study was to investigate the main pathological features of positive feline mammary carcinomas (FMCs). Twenty-four FMCs were collected at the University of Bologna, submitted to laser microdissection and analysed by nested fluorescence-PCR using primer sets specific for MMTV env sequence. For immunohistochemistry, an antibody against MMTV protein 14 (p14) was used. MMTV-like sequences were detected in three out of 24 FMCs (12.5%), one tubular carcinoma, one tubulopapillary carcinoma and one ductal carcinoma. All PCR-positive tumours were also positive for p14. Multiple nucleotide alignment has shown similarity to MMTV ranging from 98% to 100%. All the 102 examined FMCs were submitted to immunohistochemistry for molecular phenotyping. Of the nine MMTV-like positive FMCs, six were basal-like and three luminal-like. Our results demonstrate MMTV-like sequences and protein in FMCs of different geographic areas. Molecular phenotyping could contribute to understand the possible role of MMTV-like virus in FMC tumor biology. |
format | Online Article Text |
id | pubmed-8532932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85329322021-10-23 Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas Parisi, Francesca Muscatello, Luisa Vera Civita, Prospero Lessi, Francesca Menicagli, Michele Millanta, Francesca Brunetti, Barbara Benazzi, Cinzia Sarli, Giuseppe Freer, Giulia Pistello, Mauro Mazzanti, Chiara Maria Poli, Alessandro Animals (Basel) Article SIMPLE SUMMARY: Mouse mammary tumour virus-like (MMTV-like) is suspected to be involved in human breast cancer and feline mammary carcinomas (FMCs). We previously reported the identification of MMTV-like sequences and viral protein in six of 78 FMCs collected in Tuscany, Italy. To corroborate this finding, FMCs samples collected from a different geographic area were investigated. MMTV-like sequences and p14 protein were identified in three of 24 FMCs collected at the University of Bologna, one tubular carcinoma, one tubulopapillary carcinoma and one ductal carcinoma. All the examined FMCs from Pisa and Bologna were submitted to immunohistochemistry for molecular phenotype characterization. Of the nine positive FMCs, six were basal-like and three luminal-like. This study highlights the presence of MMTV-like sequences and protein in FMCs of different geographic areas. The characterization of molecular phenotype could contribute to understand the possible role of MMTV-like virus in FMC biological behaviour. ABSTRACT: In the last few years MMTV-like nucleotide sequences were detected in some feline and canine mammary tumours. Due to the confirmed role of cats in the epidemiology of the MMTV-like virus, the aim of this study was to investigate the main pathological features of positive feline mammary carcinomas (FMCs). Twenty-four FMCs were collected at the University of Bologna, submitted to laser microdissection and analysed by nested fluorescence-PCR using primer sets specific for MMTV env sequence. For immunohistochemistry, an antibody against MMTV protein 14 (p14) was used. MMTV-like sequences were detected in three out of 24 FMCs (12.5%), one tubular carcinoma, one tubulopapillary carcinoma and one ductal carcinoma. All PCR-positive tumours were also positive for p14. Multiple nucleotide alignment has shown similarity to MMTV ranging from 98% to 100%. All the 102 examined FMCs were submitted to immunohistochemistry for molecular phenotyping. Of the nine MMTV-like positive FMCs, six were basal-like and three luminal-like. Our results demonstrate MMTV-like sequences and protein in FMCs of different geographic areas. Molecular phenotyping could contribute to understand the possible role of MMTV-like virus in FMC tumor biology. MDPI 2021-09-27 /pmc/articles/PMC8532932/ /pubmed/34679842 http://dx.doi.org/10.3390/ani11102821 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Parisi, Francesca Muscatello, Luisa Vera Civita, Prospero Lessi, Francesca Menicagli, Michele Millanta, Francesca Brunetti, Barbara Benazzi, Cinzia Sarli, Giuseppe Freer, Giulia Pistello, Mauro Mazzanti, Chiara Maria Poli, Alessandro Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas |
title | Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas |
title_full | Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas |
title_fullStr | Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas |
title_full_unstemmed | Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas |
title_short | Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas |
title_sort | pathological features and molecular phenotype of mmtv like-positive feline mammary carcinomas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532932/ https://www.ncbi.nlm.nih.gov/pubmed/34679842 http://dx.doi.org/10.3390/ani11102821 |
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