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Dimeric Histidine as a Novel Free Radical Scavenger Alleviates Non-Alcoholic Liver Injury
Non-alcoholic liver injury (NLI) is a common disease worldwide. Since free radical damage in the liver is a crucial initiator leading to diseases, scavenging excess free radicals has become an essential therapeutic strategy. To enhance the antioxidant capacity of histidine, we synthesized a protonat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532998/ https://www.ncbi.nlm.nih.gov/pubmed/34679664 http://dx.doi.org/10.3390/antiox10101529 |
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author | Zhao, Zizhen Fu, Chen Zhang, Yuping Fu, Ailing |
author_facet | Zhao, Zizhen Fu, Chen Zhang, Yuping Fu, Ailing |
author_sort | Zhao, Zizhen |
collection | PubMed |
description | Non-alcoholic liver injury (NLI) is a common disease worldwide. Since free radical damage in the liver is a crucial initiator leading to diseases, scavenging excess free radicals has become an essential therapeutic strategy. To enhance the antioxidant capacity of histidine, we synthesized a protonated dimeric histidine, H-bihistidine, and investigated its anti-free radical potential in several free-radical-induced NLI. Results showed that H-bihistidine could strongly scavenge free radicals caused by H(2)O(2), fatty acid, and CCl(4), respectively, and recover cell viability in cultured hepatocytes. In the animal model of nonalcoholic fatty liver injury caused by high-fat diet, H-bihistidine reduced the contents of transaminases and lipids in serum, eliminated the liver’s fat accumulation, and decreased the oxidative damage. Moreover, H-bihistidine could rescue CCl(4)-induced liver injury and recover energy supply through scavenging free radicals. Moreover, liver fibrosis prepared by high-fat diet and CCl(4) administration was significantly alleviated after H-bihistidine treatment. This study suggests a novel nonenzymatic free radical scavenger against NLI and, potentially, other free-radical-induced diseases. |
format | Online Article Text |
id | pubmed-8532998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85329982021-10-23 Dimeric Histidine as a Novel Free Radical Scavenger Alleviates Non-Alcoholic Liver Injury Zhao, Zizhen Fu, Chen Zhang, Yuping Fu, Ailing Antioxidants (Basel) Article Non-alcoholic liver injury (NLI) is a common disease worldwide. Since free radical damage in the liver is a crucial initiator leading to diseases, scavenging excess free radicals has become an essential therapeutic strategy. To enhance the antioxidant capacity of histidine, we synthesized a protonated dimeric histidine, H-bihistidine, and investigated its anti-free radical potential in several free-radical-induced NLI. Results showed that H-bihistidine could strongly scavenge free radicals caused by H(2)O(2), fatty acid, and CCl(4), respectively, and recover cell viability in cultured hepatocytes. In the animal model of nonalcoholic fatty liver injury caused by high-fat diet, H-bihistidine reduced the contents of transaminases and lipids in serum, eliminated the liver’s fat accumulation, and decreased the oxidative damage. Moreover, H-bihistidine could rescue CCl(4)-induced liver injury and recover energy supply through scavenging free radicals. Moreover, liver fibrosis prepared by high-fat diet and CCl(4) administration was significantly alleviated after H-bihistidine treatment. This study suggests a novel nonenzymatic free radical scavenger against NLI and, potentially, other free-radical-induced diseases. MDPI 2021-09-27 /pmc/articles/PMC8532998/ /pubmed/34679664 http://dx.doi.org/10.3390/antiox10101529 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Zizhen Fu, Chen Zhang, Yuping Fu, Ailing Dimeric Histidine as a Novel Free Radical Scavenger Alleviates Non-Alcoholic Liver Injury |
title | Dimeric Histidine as a Novel Free Radical Scavenger Alleviates Non-Alcoholic Liver Injury |
title_full | Dimeric Histidine as a Novel Free Radical Scavenger Alleviates Non-Alcoholic Liver Injury |
title_fullStr | Dimeric Histidine as a Novel Free Radical Scavenger Alleviates Non-Alcoholic Liver Injury |
title_full_unstemmed | Dimeric Histidine as a Novel Free Radical Scavenger Alleviates Non-Alcoholic Liver Injury |
title_short | Dimeric Histidine as a Novel Free Radical Scavenger Alleviates Non-Alcoholic Liver Injury |
title_sort | dimeric histidine as a novel free radical scavenger alleviates non-alcoholic liver injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532998/ https://www.ncbi.nlm.nih.gov/pubmed/34679664 http://dx.doi.org/10.3390/antiox10101529 |
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