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Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation
The spheroid culture system provides an efficient method to emulate organ-specific pathophysiology, overcoming the traditional two-dimensional (2D) cell culture limitations. The intervention of microfluidics in the spheroid culture platform has the potential to enhance the capacity of in vitro micro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533112/ https://www.ncbi.nlm.nih.gov/pubmed/34680487 http://dx.doi.org/10.3390/biomedicines9101369 |
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author | Kim, Young-Su Asif, Arun Chethikkattuveli Salih, Abdul Rahim Lee, Jae-Wook Hyun, Ki-Nam Choi, Kyung-Hyun |
author_facet | Kim, Young-Su Asif, Arun Chethikkattuveli Salih, Abdul Rahim Lee, Jae-Wook Hyun, Ki-Nam Choi, Kyung-Hyun |
author_sort | Kim, Young-Su |
collection | PubMed |
description | The spheroid culture system provides an efficient method to emulate organ-specific pathophysiology, overcoming the traditional two-dimensional (2D) cell culture limitations. The intervention of microfluidics in the spheroid culture platform has the potential to enhance the capacity of in vitro microphysiological tissues for disease modeling. Conventionally, spheroid culture is carried out in static conditions, making the media nutrient-deficient around the spheroid periphery. The current approach tries to enhance the capacity of the spheroid culture platform by integrating the perfusion channel for dynamic culture conditions. A pro-inflammatory hepatic model was emulated using a coculture of HepG2 cell line, fibroblasts, and endothelial cells for validating the spheroid culture plate with a perfusable channel across the spheroid well. Enhanced proliferation and metabolic capacity of the microphysiological model were observed and further validated by metabolic assays. A comparative analysis of static and dynamic conditions validated the advantage of spheroid culture with dynamic media flow. Hepatic spheroids were found to have improved proliferation in dynamic flow conditions as compared to the static culture platform. The perfusable culture system for spheroids is more physiologically relevant as compared to the static spheroid culture system for disease and drug analysis. |
format | Online Article Text |
id | pubmed-8533112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85331122021-10-23 Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation Kim, Young-Su Asif, Arun Chethikkattuveli Salih, Abdul Rahim Lee, Jae-Wook Hyun, Ki-Nam Choi, Kyung-Hyun Biomedicines Article The spheroid culture system provides an efficient method to emulate organ-specific pathophysiology, overcoming the traditional two-dimensional (2D) cell culture limitations. The intervention of microfluidics in the spheroid culture platform has the potential to enhance the capacity of in vitro microphysiological tissues for disease modeling. Conventionally, spheroid culture is carried out in static conditions, making the media nutrient-deficient around the spheroid periphery. The current approach tries to enhance the capacity of the spheroid culture platform by integrating the perfusion channel for dynamic culture conditions. A pro-inflammatory hepatic model was emulated using a coculture of HepG2 cell line, fibroblasts, and endothelial cells for validating the spheroid culture plate with a perfusable channel across the spheroid well. Enhanced proliferation and metabolic capacity of the microphysiological model were observed and further validated by metabolic assays. A comparative analysis of static and dynamic conditions validated the advantage of spheroid culture with dynamic media flow. Hepatic spheroids were found to have improved proliferation in dynamic flow conditions as compared to the static culture platform. The perfusable culture system for spheroids is more physiologically relevant as compared to the static spheroid culture system for disease and drug analysis. MDPI 2021-10-01 /pmc/articles/PMC8533112/ /pubmed/34680487 http://dx.doi.org/10.3390/biomedicines9101369 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Young-Su Asif, Arun Chethikkattuveli Salih, Abdul Rahim Lee, Jae-Wook Hyun, Ki-Nam Choi, Kyung-Hyun Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation |
title | Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation |
title_full | Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation |
title_fullStr | Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation |
title_full_unstemmed | Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation |
title_short | Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation |
title_sort | gravity-based flow efficient perfusion culture system for spheroids mimicking liver inflammation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533112/ https://www.ncbi.nlm.nih.gov/pubmed/34680487 http://dx.doi.org/10.3390/biomedicines9101369 |
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