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Role of Lamin A/C as Candidate Biomarker of Aggressiveness and Tumorigenicity in Glioblastoma Multiforme
Nuclear lamina components have long been regarded as scaffolding proteins, forming a dense fibrillar structure necessary for the maintenance of the nucleus shape in all the animal kingdom. More recently, mutations, aberrant localisation and deregulation of these proteins have been linked to several...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533312/ https://www.ncbi.nlm.nih.gov/pubmed/34680461 http://dx.doi.org/10.3390/biomedicines9101343 |
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author | Gatti, Giuliana Vilardo, Laura Musa, Carla Di Pietro, Chiara Bonaventura, Fabrizio Scavizzi, Ferdinando Torcinaro, Alessio Bucci, Barbara Saporito, Raffaele Arisi, Ivan De Santa, Francesca Raspa, Marcello Guglielmi, Loredana D’Agnano, Igea |
author_facet | Gatti, Giuliana Vilardo, Laura Musa, Carla Di Pietro, Chiara Bonaventura, Fabrizio Scavizzi, Ferdinando Torcinaro, Alessio Bucci, Barbara Saporito, Raffaele Arisi, Ivan De Santa, Francesca Raspa, Marcello Guglielmi, Loredana D’Agnano, Igea |
author_sort | Gatti, Giuliana |
collection | PubMed |
description | Nuclear lamina components have long been regarded as scaffolding proteins, forming a dense fibrillar structure necessary for the maintenance of the nucleus shape in all the animal kingdom. More recently, mutations, aberrant localisation and deregulation of these proteins have been linked to several diseases, including cancer. Using publicly available data we found that the increased expression levels of the nuclear protein Lamin A/C correlate with a reduced overall survival in The Cancer Genome Atlas Research Network (TCGA) patients affected by glioblastoma multiforme (GBM). We show that the expression of the LMNA gene is linked to the enrichment of cancer-related pathways, particularly pathways related to cell adhesion and cell migration. Mimicking the modulation of LMNA in a GBM preclinical cancer model, we confirmed both in vitro and in vivo that the increased expression of LMNA is associated with an increased aggressiveness and tumorigenicity. In addition, delving into the possible mechanism behind LMNA-induced GBM aggressiveness and tumorigenicity, we found that the mTORC2 component, Rictor, plays a central role in mediating these effects. |
format | Online Article Text |
id | pubmed-8533312 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85333122021-10-23 Role of Lamin A/C as Candidate Biomarker of Aggressiveness and Tumorigenicity in Glioblastoma Multiforme Gatti, Giuliana Vilardo, Laura Musa, Carla Di Pietro, Chiara Bonaventura, Fabrizio Scavizzi, Ferdinando Torcinaro, Alessio Bucci, Barbara Saporito, Raffaele Arisi, Ivan De Santa, Francesca Raspa, Marcello Guglielmi, Loredana D’Agnano, Igea Biomedicines Article Nuclear lamina components have long been regarded as scaffolding proteins, forming a dense fibrillar structure necessary for the maintenance of the nucleus shape in all the animal kingdom. More recently, mutations, aberrant localisation and deregulation of these proteins have been linked to several diseases, including cancer. Using publicly available data we found that the increased expression levels of the nuclear protein Lamin A/C correlate with a reduced overall survival in The Cancer Genome Atlas Research Network (TCGA) patients affected by glioblastoma multiforme (GBM). We show that the expression of the LMNA gene is linked to the enrichment of cancer-related pathways, particularly pathways related to cell adhesion and cell migration. Mimicking the modulation of LMNA in a GBM preclinical cancer model, we confirmed both in vitro and in vivo that the increased expression of LMNA is associated with an increased aggressiveness and tumorigenicity. In addition, delving into the possible mechanism behind LMNA-induced GBM aggressiveness and tumorigenicity, we found that the mTORC2 component, Rictor, plays a central role in mediating these effects. MDPI 2021-09-28 /pmc/articles/PMC8533312/ /pubmed/34680461 http://dx.doi.org/10.3390/biomedicines9101343 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gatti, Giuliana Vilardo, Laura Musa, Carla Di Pietro, Chiara Bonaventura, Fabrizio Scavizzi, Ferdinando Torcinaro, Alessio Bucci, Barbara Saporito, Raffaele Arisi, Ivan De Santa, Francesca Raspa, Marcello Guglielmi, Loredana D’Agnano, Igea Role of Lamin A/C as Candidate Biomarker of Aggressiveness and Tumorigenicity in Glioblastoma Multiforme |
title | Role of Lamin A/C as Candidate Biomarker of Aggressiveness and Tumorigenicity in Glioblastoma Multiforme |
title_full | Role of Lamin A/C as Candidate Biomarker of Aggressiveness and Tumorigenicity in Glioblastoma Multiforme |
title_fullStr | Role of Lamin A/C as Candidate Biomarker of Aggressiveness and Tumorigenicity in Glioblastoma Multiforme |
title_full_unstemmed | Role of Lamin A/C as Candidate Biomarker of Aggressiveness and Tumorigenicity in Glioblastoma Multiforme |
title_short | Role of Lamin A/C as Candidate Biomarker of Aggressiveness and Tumorigenicity in Glioblastoma Multiforme |
title_sort | role of lamin a/c as candidate biomarker of aggressiveness and tumorigenicity in glioblastoma multiforme |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533312/ https://www.ncbi.nlm.nih.gov/pubmed/34680461 http://dx.doi.org/10.3390/biomedicines9101343 |
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