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The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine
(1) Background: CIC-DUX4 sarcoma is a rare mesenchymal small round cell tumor which belongs to rare cancers that occupy a significant percentage of cancer cases as a whole, despite each being rare. Importantly, each rare cancer type has different features, and thus there is a need to develop a model...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533847/ https://www.ncbi.nlm.nih.gov/pubmed/34685592 http://dx.doi.org/10.3390/cells10102613 |
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author | Komatsu, Aoi Matsumoto, Kotaro Yoshimatsu, Yuki Sin, Yooksil Kubota, Arisa Saito, Tomoki Mizumoto, Ayaka Ohashi, Shinya Muto, Manabu Noguchi, Rei Kondo, Tadashi Tamanoi, Fuyuhiko |
author_facet | Komatsu, Aoi Matsumoto, Kotaro Yoshimatsu, Yuki Sin, Yooksil Kubota, Arisa Saito, Tomoki Mizumoto, Ayaka Ohashi, Shinya Muto, Manabu Noguchi, Rei Kondo, Tadashi Tamanoi, Fuyuhiko |
author_sort | Komatsu, Aoi |
collection | PubMed |
description | (1) Background: CIC-DUX4 sarcoma is a rare mesenchymal small round cell tumor which belongs to rare cancers that occupy a significant percentage of cancer cases as a whole, despite each being rare. Importantly, each rare cancer type has different features, and thus there is a need to develop a model that mimics the features of each of these cancers. We evaluated the idea that the chicken chorioallantoic membrane assay (CAM), a convenient and versatile animal model, can be established for the CIC-DUX4 sarcoma. (2) Methods: Patient-derived cell lines of CIC-DUX4 were applied. These cells were transplanted onto the CAM membrane and tumor formation was examined by H&E staining, immunohistochemistry and Western blotting. The CAM tumor was transferred onto a fresh CAM and was also used to form organoids. Retention of the fusion gene was examined. (3) Results: H&E staining as well as molecular characterization demonstrated the formation of the CIC-DUX4 tumor on the CAM membrane. Expression of cyclin D2 and ETV4 was identified. The CAM tumor was transferred to a fresh CAM to form the second-generation CAM tumor. In addition, we were successful in forming tumor organoids using the CAM tumor. Retention of the fusion gene CIC-DUX4 in the CAM, second-generation CAM, and in the CAM-derived organoids was confirmed by RT-PCR. (4) Conclusions: The CAM assay provides a promising model for CIC-DUX4 sarcoma. |
format | Online Article Text |
id | pubmed-8533847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85338472021-10-23 The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine Komatsu, Aoi Matsumoto, Kotaro Yoshimatsu, Yuki Sin, Yooksil Kubota, Arisa Saito, Tomoki Mizumoto, Ayaka Ohashi, Shinya Muto, Manabu Noguchi, Rei Kondo, Tadashi Tamanoi, Fuyuhiko Cells Article (1) Background: CIC-DUX4 sarcoma is a rare mesenchymal small round cell tumor which belongs to rare cancers that occupy a significant percentage of cancer cases as a whole, despite each being rare. Importantly, each rare cancer type has different features, and thus there is a need to develop a model that mimics the features of each of these cancers. We evaluated the idea that the chicken chorioallantoic membrane assay (CAM), a convenient and versatile animal model, can be established for the CIC-DUX4 sarcoma. (2) Methods: Patient-derived cell lines of CIC-DUX4 were applied. These cells were transplanted onto the CAM membrane and tumor formation was examined by H&E staining, immunohistochemistry and Western blotting. The CAM tumor was transferred onto a fresh CAM and was also used to form organoids. Retention of the fusion gene was examined. (3) Results: H&E staining as well as molecular characterization demonstrated the formation of the CIC-DUX4 tumor on the CAM membrane. Expression of cyclin D2 and ETV4 was identified. The CAM tumor was transferred to a fresh CAM to form the second-generation CAM tumor. In addition, we were successful in forming tumor organoids using the CAM tumor. Retention of the fusion gene CIC-DUX4 in the CAM, second-generation CAM, and in the CAM-derived organoids was confirmed by RT-PCR. (4) Conclusions: The CAM assay provides a promising model for CIC-DUX4 sarcoma. MDPI 2021-10-01 /pmc/articles/PMC8533847/ /pubmed/34685592 http://dx.doi.org/10.3390/cells10102613 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Komatsu, Aoi Matsumoto, Kotaro Yoshimatsu, Yuki Sin, Yooksil Kubota, Arisa Saito, Tomoki Mizumoto, Ayaka Ohashi, Shinya Muto, Manabu Noguchi, Rei Kondo, Tadashi Tamanoi, Fuyuhiko The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine |
title | The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine |
title_full | The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine |
title_fullStr | The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine |
title_full_unstemmed | The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine |
title_short | The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine |
title_sort | cam model for cic-dux4 sarcoma and its potential use for precision medicine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533847/ https://www.ncbi.nlm.nih.gov/pubmed/34685592 http://dx.doi.org/10.3390/cells10102613 |
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