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The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine

(1) Background: CIC-DUX4 sarcoma is a rare mesenchymal small round cell tumor which belongs to rare cancers that occupy a significant percentage of cancer cases as a whole, despite each being rare. Importantly, each rare cancer type has different features, and thus there is a need to develop a model...

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Autores principales: Komatsu, Aoi, Matsumoto, Kotaro, Yoshimatsu, Yuki, Sin, Yooksil, Kubota, Arisa, Saito, Tomoki, Mizumoto, Ayaka, Ohashi, Shinya, Muto, Manabu, Noguchi, Rei, Kondo, Tadashi, Tamanoi, Fuyuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533847/
https://www.ncbi.nlm.nih.gov/pubmed/34685592
http://dx.doi.org/10.3390/cells10102613
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author Komatsu, Aoi
Matsumoto, Kotaro
Yoshimatsu, Yuki
Sin, Yooksil
Kubota, Arisa
Saito, Tomoki
Mizumoto, Ayaka
Ohashi, Shinya
Muto, Manabu
Noguchi, Rei
Kondo, Tadashi
Tamanoi, Fuyuhiko
author_facet Komatsu, Aoi
Matsumoto, Kotaro
Yoshimatsu, Yuki
Sin, Yooksil
Kubota, Arisa
Saito, Tomoki
Mizumoto, Ayaka
Ohashi, Shinya
Muto, Manabu
Noguchi, Rei
Kondo, Tadashi
Tamanoi, Fuyuhiko
author_sort Komatsu, Aoi
collection PubMed
description (1) Background: CIC-DUX4 sarcoma is a rare mesenchymal small round cell tumor which belongs to rare cancers that occupy a significant percentage of cancer cases as a whole, despite each being rare. Importantly, each rare cancer type has different features, and thus there is a need to develop a model that mimics the features of each of these cancers. We evaluated the idea that the chicken chorioallantoic membrane assay (CAM), a convenient and versatile animal model, can be established for the CIC-DUX4 sarcoma. (2) Methods: Patient-derived cell lines of CIC-DUX4 were applied. These cells were transplanted onto the CAM membrane and tumor formation was examined by H&E staining, immunohistochemistry and Western blotting. The CAM tumor was transferred onto a fresh CAM and was also used to form organoids. Retention of the fusion gene was examined. (3) Results: H&E staining as well as molecular characterization demonstrated the formation of the CIC-DUX4 tumor on the CAM membrane. Expression of cyclin D2 and ETV4 was identified. The CAM tumor was transferred to a fresh CAM to form the second-generation CAM tumor. In addition, we were successful in forming tumor organoids using the CAM tumor. Retention of the fusion gene CIC-DUX4 in the CAM, second-generation CAM, and in the CAM-derived organoids was confirmed by RT-PCR. (4) Conclusions: The CAM assay provides a promising model for CIC-DUX4 sarcoma.
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spelling pubmed-85338472021-10-23 The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine Komatsu, Aoi Matsumoto, Kotaro Yoshimatsu, Yuki Sin, Yooksil Kubota, Arisa Saito, Tomoki Mizumoto, Ayaka Ohashi, Shinya Muto, Manabu Noguchi, Rei Kondo, Tadashi Tamanoi, Fuyuhiko Cells Article (1) Background: CIC-DUX4 sarcoma is a rare mesenchymal small round cell tumor which belongs to rare cancers that occupy a significant percentage of cancer cases as a whole, despite each being rare. Importantly, each rare cancer type has different features, and thus there is a need to develop a model that mimics the features of each of these cancers. We evaluated the idea that the chicken chorioallantoic membrane assay (CAM), a convenient and versatile animal model, can be established for the CIC-DUX4 sarcoma. (2) Methods: Patient-derived cell lines of CIC-DUX4 were applied. These cells were transplanted onto the CAM membrane and tumor formation was examined by H&E staining, immunohistochemistry and Western blotting. The CAM tumor was transferred onto a fresh CAM and was also used to form organoids. Retention of the fusion gene was examined. (3) Results: H&E staining as well as molecular characterization demonstrated the formation of the CIC-DUX4 tumor on the CAM membrane. Expression of cyclin D2 and ETV4 was identified. The CAM tumor was transferred to a fresh CAM to form the second-generation CAM tumor. In addition, we were successful in forming tumor organoids using the CAM tumor. Retention of the fusion gene CIC-DUX4 in the CAM, second-generation CAM, and in the CAM-derived organoids was confirmed by RT-PCR. (4) Conclusions: The CAM assay provides a promising model for CIC-DUX4 sarcoma. MDPI 2021-10-01 /pmc/articles/PMC8533847/ /pubmed/34685592 http://dx.doi.org/10.3390/cells10102613 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Komatsu, Aoi
Matsumoto, Kotaro
Yoshimatsu, Yuki
Sin, Yooksil
Kubota, Arisa
Saito, Tomoki
Mizumoto, Ayaka
Ohashi, Shinya
Muto, Manabu
Noguchi, Rei
Kondo, Tadashi
Tamanoi, Fuyuhiko
The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine
title The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine
title_full The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine
title_fullStr The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine
title_full_unstemmed The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine
title_short The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine
title_sort cam model for cic-dux4 sarcoma and its potential use for precision medicine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533847/
https://www.ncbi.nlm.nih.gov/pubmed/34685592
http://dx.doi.org/10.3390/cells10102613
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