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Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses

The treatment for mosquito-borne viral diseases such as dengue virus (DENV), zika virus (ZIKV), and chikungunya virus (CHIKV) has become difficult due to delayed diagnosis processes. In addition, sharing the same transmission media and similar symptoms at the early stage of infection of these diseas...

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Autores principales: Nasrin, Fahmida, Tsuruga, Kenta, Utomo, Doddy Irawan Setyo, Chowdhury, Ankan Dutta, Park, Enoch Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533959/
https://www.ncbi.nlm.nih.gov/pubmed/34677332
http://dx.doi.org/10.3390/bios11100376
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author Nasrin, Fahmida
Tsuruga, Kenta
Utomo, Doddy Irawan Setyo
Chowdhury, Ankan Dutta
Park, Enoch Y.
author_facet Nasrin, Fahmida
Tsuruga, Kenta
Utomo, Doddy Irawan Setyo
Chowdhury, Ankan Dutta
Park, Enoch Y.
author_sort Nasrin, Fahmida
collection PubMed
description The treatment for mosquito-borne viral diseases such as dengue virus (DENV), zika virus (ZIKV), and chikungunya virus (CHIKV) has become difficult due to delayed diagnosis processes. In addition, sharing the same transmission media and similar symptoms at the early stage of infection of these diseases has become more critical for early diagnosis. To overcome this, a common platform that can identify the virus with high sensitivity and selectivity, even for the different serotypes, is in high demand. In this study, we have attempted an electrochemical impedimetric method to detect the ZIKV, DENV, and CHIKV using their corresponding antibody-conjugated sensor electrodes. The significance of this method is emphasized on the fabrication of a common matrix of gold–polyaniline and sulfur, nitrogen-doped graphene quantum dot nanocomposites (Au-PAni-N,S-GQDs), which have a strong impedimetric response based only on the conjugated antibody, resulting in minimum cross-reactivity for the detection of various mosquito-borne viruses, separately. As a result, four serotypes of DENV and ZIKV, and CHIKV have been detected successfully with an LOD of femtogram mL(−1).
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spelling pubmed-85339592021-10-23 Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses Nasrin, Fahmida Tsuruga, Kenta Utomo, Doddy Irawan Setyo Chowdhury, Ankan Dutta Park, Enoch Y. Biosensors (Basel) Article The treatment for mosquito-borne viral diseases such as dengue virus (DENV), zika virus (ZIKV), and chikungunya virus (CHIKV) has become difficult due to delayed diagnosis processes. In addition, sharing the same transmission media and similar symptoms at the early stage of infection of these diseases has become more critical for early diagnosis. To overcome this, a common platform that can identify the virus with high sensitivity and selectivity, even for the different serotypes, is in high demand. In this study, we have attempted an electrochemical impedimetric method to detect the ZIKV, DENV, and CHIKV using their corresponding antibody-conjugated sensor electrodes. The significance of this method is emphasized on the fabrication of a common matrix of gold–polyaniline and sulfur, nitrogen-doped graphene quantum dot nanocomposites (Au-PAni-N,S-GQDs), which have a strong impedimetric response based only on the conjugated antibody, resulting in minimum cross-reactivity for the detection of various mosquito-borne viruses, separately. As a result, four serotypes of DENV and ZIKV, and CHIKV have been detected successfully with an LOD of femtogram mL(−1). MDPI 2021-10-07 /pmc/articles/PMC8533959/ /pubmed/34677332 http://dx.doi.org/10.3390/bios11100376 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nasrin, Fahmida
Tsuruga, Kenta
Utomo, Doddy Irawan Setyo
Chowdhury, Ankan Dutta
Park, Enoch Y.
Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses
title Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses
title_full Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses
title_fullStr Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses
title_full_unstemmed Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses
title_short Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses
title_sort design and analysis of a single system of impedimetric biosensors for the detection of mosquito-borne viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533959/
https://www.ncbi.nlm.nih.gov/pubmed/34677332
http://dx.doi.org/10.3390/bios11100376
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