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High-Performance Passive Plasma Separation on OSTE Pillar Forest
Plasma separation is of high interest for lateral flow tests using whole blood as sample liquids. Here, we built a passive microfluidic device for plasma separation with high performance. This device was made by blood filtration membrane and off-stoichiometry thiol–ene (OSTE) pillar forest. OSTE pil...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534190/ https://www.ncbi.nlm.nih.gov/pubmed/34677311 http://dx.doi.org/10.3390/bios11100355 |
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author | Xiao, Zhiqing Sun, Lexin Yang, Yuqian Feng, Zitao Dai, Sihan Yang, Hao Zhang, Xingwei Sheu, Chia-Lin Guo, Weijin |
author_facet | Xiao, Zhiqing Sun, Lexin Yang, Yuqian Feng, Zitao Dai, Sihan Yang, Hao Zhang, Xingwei Sheu, Chia-Lin Guo, Weijin |
author_sort | Xiao, Zhiqing |
collection | PubMed |
description | Plasma separation is of high interest for lateral flow tests using whole blood as sample liquids. Here, we built a passive microfluidic device for plasma separation with high performance. This device was made by blood filtration membrane and off-stoichiometry thiol–ene (OSTE) pillar forest. OSTE pillar forest was fabricated by double replica moldings of a laser-cut polymethylmethacrylate (PMMA) mold, which has a uniform microstructure. This device utilized a filtration membrane to separate plasma from whole blood samples and used hydrophilic OSTE pillar forest as the capillary pump to propel the plasma. The device can be used to separate blood plasma with high purity for later use in lateral flow tests. The device can process 45 [Formula: see text] L of whole blood in 72 s and achieves a plasma separation yield as high as 60.0%. The protein recovery rate of separated plasma is 85.5%, which is on par with state-of-the-art technologies. This device can be further developed into lateral flow tests for biomarker detection in whole blood. |
format | Online Article Text |
id | pubmed-8534190 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85341902021-10-23 High-Performance Passive Plasma Separation on OSTE Pillar Forest Xiao, Zhiqing Sun, Lexin Yang, Yuqian Feng, Zitao Dai, Sihan Yang, Hao Zhang, Xingwei Sheu, Chia-Lin Guo, Weijin Biosensors (Basel) Communication Plasma separation is of high interest for lateral flow tests using whole blood as sample liquids. Here, we built a passive microfluidic device for plasma separation with high performance. This device was made by blood filtration membrane and off-stoichiometry thiol–ene (OSTE) pillar forest. OSTE pillar forest was fabricated by double replica moldings of a laser-cut polymethylmethacrylate (PMMA) mold, which has a uniform microstructure. This device utilized a filtration membrane to separate plasma from whole blood samples and used hydrophilic OSTE pillar forest as the capillary pump to propel the plasma. The device can be used to separate blood plasma with high purity for later use in lateral flow tests. The device can process 45 [Formula: see text] L of whole blood in 72 s and achieves a plasma separation yield as high as 60.0%. The protein recovery rate of separated plasma is 85.5%, which is on par with state-of-the-art technologies. This device can be further developed into lateral flow tests for biomarker detection in whole blood. MDPI 2021-09-25 /pmc/articles/PMC8534190/ /pubmed/34677311 http://dx.doi.org/10.3390/bios11100355 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Xiao, Zhiqing Sun, Lexin Yang, Yuqian Feng, Zitao Dai, Sihan Yang, Hao Zhang, Xingwei Sheu, Chia-Lin Guo, Weijin High-Performance Passive Plasma Separation on OSTE Pillar Forest |
title | High-Performance Passive Plasma Separation on OSTE Pillar Forest |
title_full | High-Performance Passive Plasma Separation on OSTE Pillar Forest |
title_fullStr | High-Performance Passive Plasma Separation on OSTE Pillar Forest |
title_full_unstemmed | High-Performance Passive Plasma Separation on OSTE Pillar Forest |
title_short | High-Performance Passive Plasma Separation on OSTE Pillar Forest |
title_sort | high-performance passive plasma separation on oste pillar forest |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534190/ https://www.ncbi.nlm.nih.gov/pubmed/34677311 http://dx.doi.org/10.3390/bios11100355 |
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