Cargando…

Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation

Steroid receptor RNA activator 1 (SRA1) is involved in pathophysiological responses of adipose tissue (AT) in obesity. In vitro and animal studies have elucidated its role in meta-inflammation. Since SRA1 AT expression in obesity/type 2 diabetes (T2D) and the relationship with immune-metabolic signa...

Descripción completa

Detalles Bibliográficos
Autores principales: Kochumon, Shihab, Arefanian, Hossein, Sindhu, Sardar, Shenouda, Steve, Thomas, Reeby, Al-Mulla, Fahd, Tuomilehto, Jaakko, Ahmad, Rasheed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534244/
https://www.ncbi.nlm.nih.gov/pubmed/34685582
http://dx.doi.org/10.3390/cells10102602
_version_ 1784587508849311744
author Kochumon, Shihab
Arefanian, Hossein
Sindhu, Sardar
Shenouda, Steve
Thomas, Reeby
Al-Mulla, Fahd
Tuomilehto, Jaakko
Ahmad, Rasheed
author_facet Kochumon, Shihab
Arefanian, Hossein
Sindhu, Sardar
Shenouda, Steve
Thomas, Reeby
Al-Mulla, Fahd
Tuomilehto, Jaakko
Ahmad, Rasheed
author_sort Kochumon, Shihab
collection PubMed
description Steroid receptor RNA activator 1 (SRA1) is involved in pathophysiological responses of adipose tissue (AT) in obesity. In vitro and animal studies have elucidated its role in meta-inflammation. Since SRA1 AT expression in obesity/type 2 diabetes (T2D) and the relationship with immune-metabolic signatures remains unclear, we assessed AT SRA1 expression and its association with immune–metabolic markers in individuals with obesity/T2D. For this, 55 non-diabetic and 53 T2D individuals classified as normal weight (NW; lean), overweight, and obese were recruited and fasting blood and subcutaneous fat biopsy samples were collected. Plasma metabolic markers were assessed using commercial kits and AT expression of SRA1 and selected immune markers using RT-qPCR. SRA1 expression was significantly higher in non-diabetic obese compared with NW individuals. SRA1 expression associated with BMI, PBF, serum insulin, and HOMA-IR in the total study population and people without diabetes. SRA1 associated with waist circumference in people without diabetes and NW participants, whereas it associated inversely with HbA1c in overweight participants. In most study subgroups AT SRA1 expression associated directly with CXCL9, CXCL10, CXCL11, TNF-α, TGF-β, IL2RA, and IL18, but inversely with CCL19 and CCR2. TGF-β/IL18 independently predicted the SRA1 expression in people without diabetes and in the total study population, while TNF-α/IL-2RA predicted SRA1 only in people with diabetes. TNF-α also predicted SRA1 in both NW and obese people regardless of the diabetes status. In conclusion, AT SRA1 expression is elevated in people with obesity which associates with typical immunometabolic markers of obesity/T2D, implying that SRA1 may have potential as a biomarker of metabolic derangements.
format Online
Article
Text
id pubmed-8534244
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85342442021-10-23 Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation Kochumon, Shihab Arefanian, Hossein Sindhu, Sardar Shenouda, Steve Thomas, Reeby Al-Mulla, Fahd Tuomilehto, Jaakko Ahmad, Rasheed Cells Article Steroid receptor RNA activator 1 (SRA1) is involved in pathophysiological responses of adipose tissue (AT) in obesity. In vitro and animal studies have elucidated its role in meta-inflammation. Since SRA1 AT expression in obesity/type 2 diabetes (T2D) and the relationship with immune-metabolic signatures remains unclear, we assessed AT SRA1 expression and its association with immune–metabolic markers in individuals with obesity/T2D. For this, 55 non-diabetic and 53 T2D individuals classified as normal weight (NW; lean), overweight, and obese were recruited and fasting blood and subcutaneous fat biopsy samples were collected. Plasma metabolic markers were assessed using commercial kits and AT expression of SRA1 and selected immune markers using RT-qPCR. SRA1 expression was significantly higher in non-diabetic obese compared with NW individuals. SRA1 expression associated with BMI, PBF, serum insulin, and HOMA-IR in the total study population and people without diabetes. SRA1 associated with waist circumference in people without diabetes and NW participants, whereas it associated inversely with HbA1c in overweight participants. In most study subgroups AT SRA1 expression associated directly with CXCL9, CXCL10, CXCL11, TNF-α, TGF-β, IL2RA, and IL18, but inversely with CCL19 and CCR2. TGF-β/IL18 independently predicted the SRA1 expression in people without diabetes and in the total study population, while TNF-α/IL-2RA predicted SRA1 only in people with diabetes. TNF-α also predicted SRA1 in both NW and obese people regardless of the diabetes status. In conclusion, AT SRA1 expression is elevated in people with obesity which associates with typical immunometabolic markers of obesity/T2D, implying that SRA1 may have potential as a biomarker of metabolic derangements. MDPI 2021-09-30 /pmc/articles/PMC8534244/ /pubmed/34685582 http://dx.doi.org/10.3390/cells10102602 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kochumon, Shihab
Arefanian, Hossein
Sindhu, Sardar
Shenouda, Steve
Thomas, Reeby
Al-Mulla, Fahd
Tuomilehto, Jaakko
Ahmad, Rasheed
Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation
title Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation
title_full Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation
title_fullStr Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation
title_full_unstemmed Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation
title_short Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation
title_sort adipose tissue steroid receptor rna activator 1 (sra1) expression is associated with obesity, insulin resistance, and inflammation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534244/
https://www.ncbi.nlm.nih.gov/pubmed/34685582
http://dx.doi.org/10.3390/cells10102602
work_keys_str_mv AT kochumonshihab adiposetissuesteroidreceptorrnaactivator1sra1expressionisassociatedwithobesityinsulinresistanceandinflammation
AT arefanianhossein adiposetissuesteroidreceptorrnaactivator1sra1expressionisassociatedwithobesityinsulinresistanceandinflammation
AT sindhusardar adiposetissuesteroidreceptorrnaactivator1sra1expressionisassociatedwithobesityinsulinresistanceandinflammation
AT shenoudasteve adiposetissuesteroidreceptorrnaactivator1sra1expressionisassociatedwithobesityinsulinresistanceandinflammation
AT thomasreeby adiposetissuesteroidreceptorrnaactivator1sra1expressionisassociatedwithobesityinsulinresistanceandinflammation
AT almullafahd adiposetissuesteroidreceptorrnaactivator1sra1expressionisassociatedwithobesityinsulinresistanceandinflammation
AT tuomilehtojaakko adiposetissuesteroidreceptorrnaactivator1sra1expressionisassociatedwithobesityinsulinresistanceandinflammation
AT ahmadrasheed adiposetissuesteroidreceptorrnaactivator1sra1expressionisassociatedwithobesityinsulinresistanceandinflammation