Cargando…

Activity of Cerebellar Nuclei Neurons Correlates with ZebrinII Identity of Their Purkinje Cell Afferents

Purkinje cells (PCs) in the cerebellar cortex can be divided into at least two main subpopulations: one subpopulation that prominently expresses ZebrinII (Z+), and shows a relatively low simple spike firing rate, and another that hardly expresses ZebrinII (Z–) and shows higher baseline firing rates....

Descripción completa

Detalles Bibliográficos
Autores principales: Beekhof, Gerrit C., Gornati, Simona V., Canto, Cathrin B., Libster, Avraham M., Schonewille, Martijn, De Zeeuw, Chris I., Hoebeek, Freek E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534335/
https://www.ncbi.nlm.nih.gov/pubmed/34685666
http://dx.doi.org/10.3390/cells10102686
_version_ 1784587528086487040
author Beekhof, Gerrit C.
Gornati, Simona V.
Canto, Cathrin B.
Libster, Avraham M.
Schonewille, Martijn
De Zeeuw, Chris I.
Hoebeek, Freek E.
author_facet Beekhof, Gerrit C.
Gornati, Simona V.
Canto, Cathrin B.
Libster, Avraham M.
Schonewille, Martijn
De Zeeuw, Chris I.
Hoebeek, Freek E.
author_sort Beekhof, Gerrit C.
collection PubMed
description Purkinje cells (PCs) in the cerebellar cortex can be divided into at least two main subpopulations: one subpopulation that prominently expresses ZebrinII (Z+), and shows a relatively low simple spike firing rate, and another that hardly expresses ZebrinII (Z–) and shows higher baseline firing rates. Likewise, the complex spike responses of PCs, which are evoked by climbing fiber inputs and thus reflect the activity of the inferior olive (IO), show the same dichotomy. However, it is not known whether the target neurons of PCs in the cerebellar nuclei (CN) maintain this bimodal distribution. Electrophysiological recordings in awake adult mice show that the rate of action potential firing of CN neurons that receive input from Z+ PCs was consistently lower than that of CN neurons innervated by Z– PCs. Similar in vivo recordings in juvenile and adolescent mice indicated that the firing frequency of CN neurons correlates to the ZebrinII identity of the PC afferents in adult, but not postnatal stages. Finally, the spontaneous action potential firing pattern of adult CN neurons recorded in vitro revealed no significant differences in intrinsic pacemaking activity between ZebrinII identities. Our findings indicate that all three main components of the olivocerebellar loop, i.e., PCs, IO neurons and CN neurons, operate at a higher rate in the Z– modules.
format Online
Article
Text
id pubmed-8534335
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85343352021-10-23 Activity of Cerebellar Nuclei Neurons Correlates with ZebrinII Identity of Their Purkinje Cell Afferents Beekhof, Gerrit C. Gornati, Simona V. Canto, Cathrin B. Libster, Avraham M. Schonewille, Martijn De Zeeuw, Chris I. Hoebeek, Freek E. Cells Article Purkinje cells (PCs) in the cerebellar cortex can be divided into at least two main subpopulations: one subpopulation that prominently expresses ZebrinII (Z+), and shows a relatively low simple spike firing rate, and another that hardly expresses ZebrinII (Z–) and shows higher baseline firing rates. Likewise, the complex spike responses of PCs, which are evoked by climbing fiber inputs and thus reflect the activity of the inferior olive (IO), show the same dichotomy. However, it is not known whether the target neurons of PCs in the cerebellar nuclei (CN) maintain this bimodal distribution. Electrophysiological recordings in awake adult mice show that the rate of action potential firing of CN neurons that receive input from Z+ PCs was consistently lower than that of CN neurons innervated by Z– PCs. Similar in vivo recordings in juvenile and adolescent mice indicated that the firing frequency of CN neurons correlates to the ZebrinII identity of the PC afferents in adult, but not postnatal stages. Finally, the spontaneous action potential firing pattern of adult CN neurons recorded in vitro revealed no significant differences in intrinsic pacemaking activity between ZebrinII identities. Our findings indicate that all three main components of the olivocerebellar loop, i.e., PCs, IO neurons and CN neurons, operate at a higher rate in the Z– modules. MDPI 2021-10-07 /pmc/articles/PMC8534335/ /pubmed/34685666 http://dx.doi.org/10.3390/cells10102686 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Beekhof, Gerrit C.
Gornati, Simona V.
Canto, Cathrin B.
Libster, Avraham M.
Schonewille, Martijn
De Zeeuw, Chris I.
Hoebeek, Freek E.
Activity of Cerebellar Nuclei Neurons Correlates with ZebrinII Identity of Their Purkinje Cell Afferents
title Activity of Cerebellar Nuclei Neurons Correlates with ZebrinII Identity of Their Purkinje Cell Afferents
title_full Activity of Cerebellar Nuclei Neurons Correlates with ZebrinII Identity of Their Purkinje Cell Afferents
title_fullStr Activity of Cerebellar Nuclei Neurons Correlates with ZebrinII Identity of Their Purkinje Cell Afferents
title_full_unstemmed Activity of Cerebellar Nuclei Neurons Correlates with ZebrinII Identity of Their Purkinje Cell Afferents
title_short Activity of Cerebellar Nuclei Neurons Correlates with ZebrinII Identity of Their Purkinje Cell Afferents
title_sort activity of cerebellar nuclei neurons correlates with zebrinii identity of their purkinje cell afferents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534335/
https://www.ncbi.nlm.nih.gov/pubmed/34685666
http://dx.doi.org/10.3390/cells10102686
work_keys_str_mv AT beekhofgerritc activityofcerebellarnucleineuronscorrelateswithzebriniiidentityoftheirpurkinjecellafferents
AT gornatisimonav activityofcerebellarnucleineuronscorrelateswithzebriniiidentityoftheirpurkinjecellafferents
AT cantocathrinb activityofcerebellarnucleineuronscorrelateswithzebriniiidentityoftheirpurkinjecellafferents
AT libsteravrahamm activityofcerebellarnucleineuronscorrelateswithzebriniiidentityoftheirpurkinjecellafferents
AT schonewillemartijn activityofcerebellarnucleineuronscorrelateswithzebriniiidentityoftheirpurkinjecellafferents
AT dezeeuwchrisi activityofcerebellarnucleineuronscorrelateswithzebriniiidentityoftheirpurkinjecellafferents
AT hoebeekfreeke activityofcerebellarnucleineuronscorrelateswithzebriniiidentityoftheirpurkinjecellafferents